##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926972_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 699199 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.704511877162297 31.0 30.0 33.0 25.0 34.0 2 30.087974954197588 31.0 30.0 33.0 26.0 34.0 3 30.171672156281687 31.0 30.0 34.0 26.0 34.0 4 33.77371392121556 35.0 35.0 37.0 28.0 37.0 5 33.723710989289174 37.0 35.0 37.0 28.0 37.0 6 33.673760975058606 37.0 35.0 37.0 29.0 37.0 7 33.6616757174996 37.0 35.0 37.0 28.0 37.0 8 33.697184921603146 37.0 35.0 37.0 29.0 37.0 9 35.230290661170855 39.0 35.0 39.0 29.0 39.0 10 35.1174815753455 39.0 35.0 39.0 29.0 39.0 11 35.16059948598325 39.0 35.0 39.0 29.0 39.0 12 35.04091539032522 39.0 35.0 39.0 28.0 39.0 13 35.05990998270879 39.0 35.0 39.0 29.0 39.0 14 36.131245897090814 40.0 36.0 41.0 28.0 41.0 15 36.12344697289327 40.0 36.0 41.0 28.0 41.0 16 36.11769038571279 40.0 36.0 41.0 28.0 41.0 17 36.08870579048311 40.0 36.0 41.0 28.0 41.0 18 36.06606988854389 40.0 36.0 41.0 28.0 41.0 19 36.12207969404991 40.0 36.0 41.0 28.0 41.0 20 36.08937226740885 40.0 36.0 41.0 27.0 41.0 21 36.03956670418579 40.0 36.0 41.0 28.0 41.0 22 35.955968186453354 40.0 36.0 41.0 27.0 41.0 23 35.875142842023514 40.0 36.0 41.0 27.0 41.0 24 35.80547741057982 40.0 36.0 41.0 27.0 41.0 25 35.697077656003515 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03963523662078572 1101 2 0.1263071160199054 1101 3 0.20365168624463337 1101 4 0.19360364059244262 1101 5 0.144801200539888 1101 6 0.13724225505811916 1101 7 0.13329600568899025 1101 8 0.17199166800306642 1101 9 0.17738731700269028 1101 10 0.25394245076630284 1101 11 0.33669541252843516 1101 12 0.11918863881577835 1101 13 0.06135599111682666 1101 14 -0.044957189967220756 1101 15 -0.03323142286088654 1101 16 0.08033114049332823 1101 17 0.025015104984831282 1101 18 0.015699274863763435 1101 19 0.040346140843247724 1101 20 0.15565766039817674 1101 21 0.057337501614320274 1101 22 0.038985086283695125 1101 23 -0.037369717363255006 1101 24 -0.07038648340779474 1101 25 0.024533991973477498 1102 1 -0.5221328714363018 1102 2 -0.507562325796556 1102 3 -0.25708625131449736 1102 4 -0.1384682610158805 1102 5 -0.25917230939388247 1102 6 -0.31781923973847626 1102 7 -0.15431042761280622 1102 8 -0.12034513426751658 1102 9 -0.18968931497460773 1102 10 -0.06583049152320086 1102 11 -0.17418443609975753 1102 12 -0.15801098275470338 1102 13 0.059463843529314886 1102 14 -0.09699124862379449 1102 15 -0.4438274493509482 1102 16 -0.3463481404905906 1102 17 -0.12919492339737815 1102 18 -0.25771605153169475 1102 19 -0.43836719879724484 1102 20 -0.4819960765933118 1102 21 -0.481924560826549 1102 22 -0.3952627850502708 1102 23 -0.3088928961712014 1102 24 -0.32204211254686754 1102 25 -0.31983686895367924 1103 1 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9 0.5613858851072138 2208 10 0.45758526103162467 2208 11 0.6315346225994318 2208 12 0.4659221272711207 2208 13 0.5770599637744454 2208 14 0.45858845596253417 2208 15 0.6185588726380189 2208 16 0.5960700582189986 2208 17 0.7127911497145192 2208 18 0.7512281812536017 2208 19 0.6977097485379815 2208 20 0.7715244259338192 2208 21 0.8235396545714408 2208 22 0.8083518986968627 2208 23 0.6420203633513353 2208 24 0.9103690119172398 2208 25 0.821059088498572 2209 1 0.18211896120573812 2209 2 0.21381077872612053 2209 3 0.5291655177403847 2209 4 0.3736661450499881 2209 5 0.39169294855945225 2209 6 0.41236412328319005 2209 7 0.4412279880530079 2209 8 0.2810926229275026 2209 9 0.3535306183963627 2209 10 0.3896666612329156 2209 11 0.4193295019474448 2209 12 0.34845643269370896 2209 13 0.4605810870775855 2209 14 0.5116237801150234 2209 15 0.4919761591343246 2209 16 0.7109017836774285 2209 17 0.5613882481753834 2209 18 0.621704652275028 2209 19 0.551933181780484 2209 20 0.627851597792322 2209 21 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0.572856980575672 2212 20 0.5373315758399073 2212 21 0.624647476173962 2212 22 0.5827844584651132 2212 23 0.5531733891686699 2212 24 0.5314685566916921 2212 25 0.5425804331647939 2213 1 0.13550449789813968 2213 2 0.19595672046819246 2213 3 0.3309885755772157 2213 4 0.24302003494850055 2213 5 0.19837335462803196 2213 6 -0.0020221290362343325 2213 7 0.08706027660273463 2213 8 0.0631177279829842 2213 9 0.09019181636713824 2213 10 -0.005154077781334365 2213 11 0.28590924261822437 2213 12 0.20116622325141265 2213 13 0.11796370713217641 2213 14 0.08560679770479851 2213 15 0.10423367978521725 2213 16 0.29006931602193475 2213 17 0.02553167288449032 2213 18 0.06983442992120104 2213 19 -0.011171349913674078 2213 20 0.09061073397717934 2213 21 0.05756386282877912 2213 22 -0.1911546192145579 2213 23 0.01072333450829177 2213 24 -0.1031508181670091 2213 25 0.08413083684639844 2214 1 0.31278622294271585 2214 2 0.2467651462290199 2214 3 0.5365130701222327 2214 4 0.16250309578443023 2214 5 0.23845842821744156 2214 6 6.169655269161467E-4 2214 7 0.07049057065816555 2214 8 0.09697943429021194 2214 9 -0.06855353707358347 2214 10 -0.2165604363899334 2214 11 -0.15889718852203316 2214 12 0.12889976007633663 2214 13 -0.10482105517554174 2214 14 -0.006889953481390876 2214 15 -0.17786082291960525 2214 16 -0.00196504585058932 2214 17 -0.12047228613511862 2214 18 -0.019531371402507602 2214 19 -0.11924427445052999 2214 20 -0.07797852529327542 2214 21 -0.1881099260436514 2214 22 0.10540843799478239 2214 23 -0.12462300415410255 2214 24 -0.2242613080579119 2214 25 -0.30182068782947624 2215 1 0.6240589148708189 2215 2 0.4143459698675045 2215 3 0.3543555799167706 2215 4 0.5680396271718138 2215 5 0.5105559549094281 2215 6 0.7107516965135474 2215 7 0.4071109313364545 2215 8 0.4384021097694699 2215 9 0.37130126449353895 2215 10 0.44028452577772725 2215 11 0.23849492201419764 2215 12 0.43095810861239414 2215 13 0.3754081818971642 2215 14 0.21681806458242647 2215 15 0.34042505799076395 2215 16 0.38642580859209374 2215 17 0.1933604440739316 2215 18 0.17418591782799098 2215 19 0.3327198670693434 2215 20 0.23176367645927343 2215 21 0.3166692108406366 2215 22 0.42943047944586965 2215 23 0.3214449744324881 2215 24 0.28148158155538994 2215 25 0.4308033514084926 2216 1 -0.03418548351174877 2216 2 0.011751883629550264 2216 3 -1.6291810059166743 2216 4 -1.3217807238442205 2216 5 -0.536187902463368 2216 6 -0.24003290184708703 2216 7 0.019407458368156938 2216 8 -0.10421419966203871 2216 9 -0.20756768384634938 2216 10 -0.2888194991662374 2216 11 -0.46588215757596885 2216 12 -0.07156443090509157 2216 13 -0.08910487480418539 2216 14 -0.40487845922852017 2216 15 -0.23332059303454855 2216 16 -0.17051058077543502 2216 17 -0.3659408979830445 2216 18 -0.37631740146439796 2216 19 -0.34163596322152756 2216 20 -0.1782879859475628 2216 21 -0.15636810464665984 2216 22 -0.35128917473731036 2216 23 -0.3253082384600319 2216 24 -0.5513391153081315 2216 25 -0.4448357187755434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17689.0 3 13.0 4 279.0 5 1846.0 6 2633.0 7 1787.0 8 1443.0 9 1228.0 10 1167.0 11 978.0 12 951.0 13 906.0 14 886.0 15 832.0 16 949.0 17 995.0 18 1068.0 19 1313.0 20 1673.0 21 2168.0 22 2710.0 23 3381.0 24 3907.0 25 4765.0 26 5586.0 27 6348.0 28 7393.0 29 8764.0 30 11429.0 31 14757.0 32 19096.0 33 25037.0 34 33654.0 35 46973.0 36 68968.0 37 119684.0 38 275943.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.587788838888784 19.336724686194863 10.324822498042073 28.750663976874275 2 13.717635200531467 20.679825440550708 41.08833349559879 24.514205863319035 3 18.739745465833003 24.430931465738592 28.964025627931527 27.86529744049688 4 11.79249398239986 17.224423947974753 38.20300086241542 32.78008120720997 5 12.546520242920769 38.88749038128721 35.76888199806053 12.797107377731502 6 31.80241477741596 38.20919555736065 17.711429362809326 12.276960302414059 7 28.611340393481928 31.442075643631775 21.525060761815116 18.42152320107118 8 24.545539066690505 39.45088503486501 19.504094403718934 16.499481494725547 9 26.172964529998882 15.677574571679498 19.83090315622649 38.31855774209513 10 14.210890658611378 30.140395441438965 34.52803904068836 21.120674859261293 11 34.322137533415045 22.730386849380903 24.77669664068791 18.17077897651614 12 23.62077694730248 24.978332317884206 31.882528268101453 19.51836246671186 13 29.318228300340536 22.540843515309795 25.480518254616392 22.660409929733277 14 19.889788014267776 23.35962059388042 27.418985752247576 29.33160563960423 15 22.066961201748903 33.070549685989754 22.776076581028374 22.08641253123297 16 21.03456561287887 29.025217679836153 29.085716083708054 20.85450062357693 17 20.745453011231422 29.994465095058775 28.31153934716726 20.94854254654254 18 20.5413074445506 28.331254755461988 30.937676988998792 20.189760810988624 19 23.183275695868698 27.31558156630883 27.52053066513348 21.980612072688995 20 23.03652491297122 28.211912573620484 28.52970706270363 20.221855450704666 21 21.844483129047163 27.351412742936997 27.3811652393574 23.422938888658436 22 22.33627336423536 29.59615216840985 27.271349072295585 20.796225395059203 23 21.013105033345393 29.190485657118092 28.249549125639838 21.546860183896673 24 22.55581029149069 27.354301136014207 29.26820547512225 20.82168309737285 25 21.624907389661395 28.458620699519145 28.588346687338916 21.328125223480544 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 212.0 1 212.0 2 217.0 3 222.0 4 222.0 5 222.0 6 361.0 7 500.0 8 500.0 9 500.0 10 850.0 11 1200.0 12 1200.0 13 1200.0 14 1984.5 15 2769.0 16 2769.0 17 2769.0 18 5037.5 19 7306.0 20 7306.0 21 7306.0 22 12059.5 23 16813.0 24 16813.0 25 16813.0 26 25107.0 27 33401.0 28 33401.0 29 33401.0 30 44847.0 31 56293.0 32 56293.0 33 56293.0 34 64480.0 35 72667.0 36 72667.0 37 72667.0 38 78600.5 39 84534.0 40 84534.0 41 84534.0 42 88102.0 43 91670.0 44 91670.0 45 91670.0 46 93562.0 47 95454.0 48 95454.0 49 95454.0 50 90336.0 51 85218.0 52 85218.0 53 85218.0 54 73350.5 55 61483.0 56 61483.0 57 61483.0 58 51720.0 59 41957.0 60 41957.0 61 41957.0 62 33594.5 63 25232.0 64 25232.0 65 25232.0 66 19103.5 67 12975.0 68 12975.0 69 12975.0 70 9380.5 71 5786.0 72 5786.0 73 5786.0 74 4025.5 75 2265.0 76 2265.0 77 2265.0 78 1575.5 79 886.0 80 886.0 81 886.0 82 576.5 83 267.0 84 267.0 85 267.0 86 168.0 87 69.0 88 69.0 89 69.0 90 43.5 91 18.0 92 18.0 93 18.0 94 10.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10812372443324432 2 0.10798070363372947 3 0.016876454342755066 4 0.0 5 0.005863852780109811 6 0.3441080436327855 7 0.022597286323350004 8 0.010583539164100635 9 0.010440518364585762 10 0.004433644784961077 11 0.005434790381565191 12 1.4302079951487346E-4 13 8.581247970892407E-4 14 1.4302079951487346E-4 15 0.002574374391267722 16 0.0010011455966041141 17 0.0 18 4.2906239854462034E-4 19 1.4302079951487346E-4 20 7.151039975743672E-4 21 0.0140160383524576 22 0.0 23 2.8604159902974693E-4 24 0.0 25 0.004719686383990823 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 699199.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.21945741587425 #Duplication Level Percentage of deduplicated Percentage of total 1 92.78617141304504 77.2161484068991 2 5.545923144191081 9.23057429859442 3 0.8175666448183171 2.0411235774929137 4 0.2701848290388669 0.8993853949846099 5 0.14423013173774338 0.6001376653117533 6 0.09828912215239882 0.490774044924313 7 0.0636234277505116 0.37062949954349556 8 0.03865789076686334 0.2573670955568401 9 0.027746087141968547 0.20781128856313685 >10 0.17195399696247293 2.7166290930724815 >50 0.01774087803799455 1.0158315986847644 >100 0.015501145120426857 2.6351769702240997 >500 0.0012056446181833585 0.6738083723006072 >1k 0.0012056446181833585 1.6446026938475147 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2089 0.29877045018657067 No Hit CTGTAGGACGTGGAATATGGCAAGA 2007 0.287042744626351 No Hit GTCCTACAGTGTGCATTTCTCATTT 1686 0.24113306798207662 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1543 0.22068109365144975 No Hit CTTTAGGACGTGAAATATGGCGAGG 1435 0.2052348473038434 No Hit GTATCAACGCAGAGTACTTTTTTTT 1360 0.1945082873402279 No Hit CTGTAGGACCTGGAATATGGCGAGA 1354 0.19365016254313866 No Hit GGTATCAACGCAGAGTACTTTTTTT 836 0.1195653883944342 No Hit GTACATGGGAAGCAGTGGTATCAAC 733 0.10483424604440224 No Hit TATCAACGCAGAGTACTTTTTTTTT 711 0.10168778845507502 No Hit CTGAAGGACCTGGAATATGGCGAGA 709 0.10140174685604526 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.4302079951487344E-4 0.0 0.0 0.0 6 0.0 1.4302079951487344E-4 0.0 0.0 0.0 7 0.0 1.4302079951487344E-4 0.0 0.0 0.0 8 0.0 1.4302079951487344E-4 0.0 0.0 0.0 9 0.0 1.4302079951487344E-4 0.0 0.0 0.0 10 0.0 1.4302079951487344E-4 0.0 0.0 0.0 11 0.0 1.4302079951487344E-4 0.0 0.0 0.0 12 0.0 1.4302079951487344E-4 0.0 0.0 0.0 13 0.0 1.4302079951487344E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTTATA 35 0.002124803 16.335512 2 TAGGACC 450 0.0 15.439385 4 TAGACTC 40 0.005211479 14.276144 5 CCAACGA 60 2.5910951E-5 14.234284 19 TGCGCCA 40 0.005314018 14.234283 12 TCCAACG 75 9.771629E-7 13.917967 18 TAGGACT 135 1.8189894E-12 13.3949 4 ATCCCGT 50 0.0015098025 13.2862835 10 CCCGTTT 50 0.0015106166 13.285334 12 GGACCTG 565 0.0 13.139106 6 GTAGGAC 1055 0.0 12.540451 3 CTGTAGG 1105 0.0 12.505079 1 AGGACCT 665 0.0 12.451374 5 GACGTGA 450 0.0 12.44627 7 GTCCTAT 115 5.0604285E-9 12.430085 1 TGTAGGA 1135 0.0 12.341587 2 GTTACAC 70 1.0700406E-4 12.237573 3 GTCTTAC 55 0.002994605 12.128751 1 TGAATAG 55 0.0030237306 12.113092 5 GACAGTC 55 0.003082711 12.081896 7 >>END_MODULE