FastQCFastQC Report
Thu 26 May 2016
SRR1926971_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926971_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596514
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11290.18926630389228083No Hit
GTCCTAAAGTGTGTATTTCTCATTT10000.16764065889484572No Hit
CTGTAGGACGTGGAATATGGCAAGA9880.16562897098810758No Hit
GTATCAACGCAGAGTACTTTTTTTT9100.1525529995943096No Hit
GTCCTACAGTGTGCATTTCTCATTT8210.13763298095266835No Hit
CTTTAGGACGTGAAATATGGCGAGG7310.12254532165213222No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG307.804738E-418.96739418
GAGTAGG456.551924E-414.8369871
TTAACCT400.00519657914.281814
GAGCACC400.00533449514.22554611
GCAAGAC759.297746E-713.9785543
TAGGACC3150.013.9039844
TAGGACT1352.7284841E-1112.69494154
CCTACAC1001.3772114E-712.390083
CTAGGAC1001.3772114E-712.390083
GTCCTAA2100.012.2632241
GTCCTAC6950.012.0769721
GTATTAT802.7447091E-511.9225791
TAGGGCT657.862173E-411.7184094
TCCTACA7250.011.5694322
GGCGAGG1850.011.27790819
CTGCGTT1354.825779E-911.23993611
CCTACAG7300.011.2281033
GCGTTGA1105.059046E-711.20800513
CTACACC855.1905627E-511.201424
GTAGGAC7600.011.1611043
Produced by FastQC (version 0.11.5)