##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1926971_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	596514
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.154039972238706	31.0	27.0	31.0	16.0	33.0
2	27.80963397338537	31.0	28.0	31.0	16.0	34.0
3	27.624929171821616	31.0	28.0	31.0	12.0	34.0
4	31.279829140640455	35.0	32.0	37.0	17.0	37.0
5	31.066788038503706	35.0	32.0	37.0	16.0	37.0
6	30.756604203757163	35.0	31.0	37.0	11.0	37.0
7	30.831767569579256	35.0	32.0	37.0	11.0	37.0
8	30.46931002457612	35.0	30.0	37.0	10.0	37.0
9	32.60703520789118	37.0	32.0	39.0	15.0	39.0
10	32.74610822210376	37.0	33.0	39.0	13.0	39.0
11	32.90408272060672	37.0	33.0	39.0	15.0	39.0
12	32.763024840992834	37.0	33.0	39.0	11.0	39.0
13	32.88695487448744	37.0	33.0	39.0	11.0	39.0
14	33.87190744894504	39.0	33.0	40.0	11.0	41.0
15	33.43419433575742	38.0	33.0	40.0	10.0	41.0
16	33.5185511153133	38.0	33.0	40.0	10.0	41.0
17	33.738648212782934	39.0	33.0	40.0	10.0	41.0
18	33.78572003339402	39.0	33.0	40.0	10.0	41.0
19	33.163766483267786	39.0	32.0	40.0	7.0	41.0
20	33.430896843996955	39.0	33.0	40.0	2.0	41.0
21	33.42970994813198	39.0	33.0	40.0	2.0	41.0
22	33.45366412188147	39.0	33.0	40.0	2.0	41.0
23	33.43261013153086	39.0	34.0	40.0	2.0	41.0
24	32.12215639532349	38.0	31.0	40.0	2.0	41.0
25	32.69203405117064	39.0	32.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.8020921512374457
1101	2	-0.6445891927399607
1101	3	-1.7509936526199965
1101	4	-1.2086905937000019
1101	5	-1.102835775135869
1101	6	-3.300032727077639
1101	7	-0.9779290057538468
1101	8	-0.5001920093861756
1101	9	-0.7887139044304519
1101	10	-9.345110286238366
1101	11	-4.463527302096516
1101	12	-2.168985368790235
1101	13	-1.19817628303052
1101	14	-1.2201361596789226
1101	15	-2.8124439306369418
1101	16	-1.233314648867399
1101	17	-1.0789479250579177
1101	18	-0.8106156591647036
1101	19	-1.778135516940658
1101	20	-1.1417508481630847
1101	21	-1.2718690571686508
1101	22	-1.05052704536525
1101	23	-1.2903358136147673
1101	24	-1.521249530181212
1101	25	-1.4891077107089643
1102	1	-0.8051571389125272
1102	2	-0.6709524179834325
1102	3	-0.8093659989406738
1102	4	-0.5809426462608478
1102	5	-0.5469073934264337
1102	6	-1.9369692680747157
1102	7	-0.5154216012480859
1102	8	-0.12811065854954506
1102	9	-0.07359597374299653
1102	10	-0.0010393048945331884
1102	11	-0.40030269557036036
1102	12	-0.6281042166809812
1102	13	-0.8142553663367806
1102	14	-0.7899002983040049
1102	15	-0.3686003392764903
1102	16	-0.17993596492674158
1102	17	-0.724250124434981
1102	18	-0.7677873608952908
1102	19	-0.10828029493287517
1102	20	-0.5010193785358936
1102	21	-0.6985265518023738
1102	22	-0.4799616277511234
1102	23	-0.9964727953789847
1102	24	-4.303974260911982
1102	25	-2.6058332949483543
1103	1	-0.5672437815483065
1103	2	-0.5754688258883469
1103	3	-0.1413713983067062
1103	4	-0.31480774299948777
1103	5	-0.6595850340854774
1103	6	0.2500014752481192
1103	7	0.08132462349293945
1103	8	0.14120547230924885
1103	9	0.11384620299018877
1103	10	-0.04783633827656786
1103	11	-0.08933359025744636
1103	12	-0.7063558433158335
1103	13	-0.3731959176990145
1103	14	-0.19640607936679544
1103	15	-0.3388873954592171
1103	16	-4.5128082503964606E-4
1103	17	-0.40948680170152585
1103	18	-0.5348752421496812
1103	19	-2.024698816578365
1103	20	-0.6298104362150738
1103	21	-0.424961200767747
1103	22	-0.464405589106228
1103	23	-0.5226253426360756
1103	24	0.8963862023819331
1103	25	0.16349926656548774
1104	1	-0.7188466175681825
1104	2	-0.21731021885081603
1104	3	-1.5569220386331288
1104	4	-0.42184527380383585
1104	5	-0.402202090603879
1104	6	-1.4334156863080558
1104	7	-0.015805017358673012
1104	8	0.39401321278515056
1104	9	0.38523249960069705
1104	10	0.25825282263321725
1104	11	0.3108199264880014
1104	12	0.11833712319327816
1104	13	-0.02046594557442205
1104	14	-0.1571532615265312
1104	15	0.4589906395264407
1104	16	-0.00468814100360504
1104	17	0.015562687249307317
1104	18	-0.19914825956171
1104	19	-1.1342814934256182
1104	20	-0.24310983426783395
1104	21	-0.018116575873790453
1104	22	0.09433476559825493
1104	23	0.03566052170359768
1104	24	1.281773832164184
1104	25	0.6485010845269485
1105	1	-0.35001067636381933
1105	2	-0.38602169348334314
1105	3	-0.03825205285788158
1105	4	-0.27067765882043204
1105	5	-0.36438796068448553
1105	6	-0.8669149750705003
1105	7	-0.29120212499262266
1105	8	0.02336954279937231
1105	9	-0.9804028726136202
1105	10	-0.6301125163900139
1105	11	-0.882844132307639
1105	12	-0.9252558118470269
1105	13	-0.7516181504108346
1105	14	-1.0848737451680677
1105	15	-0.45026730262623715
1105	16	-1.124561303639922
1105	17	-1.25073650668147
1105	18	-0.9763530959770748
1105	19	-1.9484410477167486
1105	20	-1.2262475772740018
1105	21	-1.0372310851198847
1105	22	-1.0409485440271595
1105	23	-1.2120872260441473
1105	24	-5.435983398750942
1105	25	-3.2876060752644207
1106	1	-0.6547770169941565
1106	2	-1.166325605919301
1106	3	-0.6302796883182147
1106	4	-1.034776789390218
1106	5	-0.6990409648763816
1106	6	-0.3632470663606746
1106	7	-2.74638183311248
1106	8	-0.6292368071462882
1106	9	-0.842982441003624
1106	10	-0.3713114761788674
1106	11	-0.6402284670382699
1106	12	-0.5437253817895851
1106	13	-0.5608868974997989
1106	14	-0.8176112859226095
1106	15	-0.140377533956773
1106	16	-0.19924885215723975
1106	17	-0.8998981275477931
1106	18	-1.0142742262363527
1106	19	-0.4617250874621348
1106	20	-0.5249104401834828
1106	21	-0.5796449183755854
1106	22	-0.7868439795185438
1106	23	-0.9156270490529934
1106	24	-3.932897697772834
1106	25	-2.169514940347483
1107	1	0.009343552324228455
1107	2	-0.24649815812413323
1107	3	0.2872314798800488
1107	4	0.2567556857469633
1107	5	0.1306971373326995
1107	6	0.1289635285524824
1107	7	-2.7267595684946535
1107	8	-1.8641833509076804
1107	9	-0.7075509188230598
1107	10	-0.020707968467377214
1107	11	-0.43782199184442305
1107	12	-0.040062071091725215
1107	13	-0.21526893960366777
1107	14	-0.36197240831472755
1107	15	-1.249027811154761
1107	16	-0.765844841994884
1107	17	-0.36812542989665786
1107	18	-0.6999366680342192
1107	19	0.16124669217727217
1107	20	0.20888858891522233
1107	21	-0.027018620928451753
1107	22	-0.2454260197335003
1107	23	-0.6531224448274955
1107	24	-4.772260992414932
1107	25	-2.534919262180484
1108	1	0.5215923508119609
1108	2	0.16985635949223266
1108	3	0.6114154164690326
1108	4	0.3532975160448366
1108	5	0.7520551077709641
1108	6	-3.186617417450961
1108	7	-1.120600989801705
1108	8	0.27823567053701836
1108	9	-0.032604455000964094
1108	10	0.5433982621904221
1108	11	0.3293090038006028
1108	12	0.5048339141018303
1108	13	0.23197031421477732
1108	14	0.29644003765500315
1108	15	-0.5974407919049867
1108	16	0.3410031385112049
1108	17	0.3744068255052113
1108	18	0.1761093509900178
1108	19	0.32988855897973224
1108	20	-1.240960626236273
1108	21	-0.3888034392077131
1108	22	-0.020014878163152616
1108	23	0.03028750162469862
1108	24	-0.6814541016519833
1108	25	-0.09023765421179064
1109	1	0.4022800823801731
1109	2	0.38837349033607893
1109	3	-0.8355199040144363
1109	4	0.32491263059738174
1109	5	0.446093657373396
1109	6	0.8874011496361689
1109	7	0.8054637035915775
1109	8	1.0943202076781304
1109	9	0.6038802875160059
1109	10	0.7121533792407817
1109	11	0.4693670086019637
1109	12	-0.020678484771380568
1109	13	0.0983511198986946
1109	14	-0.04747431506414301
1109	15	-1.2512506527212182
1109	16	-0.08534602819015191
1109	17	-0.1685625207612489
1109	18	-0.07210401786778675
1109	19	-2.2291916902466404
1109	20	-0.509191202260034
1109	21	-0.20890529452875484
1109	22	-0.105613590401056
1109	23	-0.23172629186155547
1109	24	-0.6087121952645163
1109	25	-0.13906992597399892
1110	1	0.5290113467791429
1110	2	0.5654480713542824
1110	3	0.7555516052234914
1110	4	0.44752409556553374
1110	5	0.8386355555529157
1110	6	1.049462404548759
1110	7	1.1419772329767675
1110	8	1.4327807036803009
1110	9	0.9873649512012364
1110	10	1.1631358971971437
1110	11	0.639351649700572
1110	12	0.7788268682574895
1110	13	0.666494951492858
1110	14	0.580957721797219
1110	15	1.1034654334693172
1110	16	0.5550252958374884
1110	17	0.295484098021781
1110	18	0.3220324956615954
1110	19	-1.4515998756376156
1110	20	0.019464442136225557
1110	21	-0.18489345914003508
1110	22	0.24629949628639025
1110	23	0.31238085906223745
1110	24	-0.6437101624687465
1110	25	-0.11435112938909242
1111	1	1.0827471736752905
1111	2	0.6113034266418111
1111	3	1.3067306267711274
1111	4	1.3068659214678675
1111	5	1.3516138272247744
1111	6	1.377847009508784
1111	7	1.3850815379864834
1111	8	1.7811567257248413
1111	9	0.9350904785988021
1111	10	0.8512191966505469
1111	11	0.921596307965018
1111	12	1.0016145088591983
1111	13	0.8234041984549592
1111	14	0.7985978717723725
1111	15	-0.3913780412562531
1111	16	-0.01740547617316679
1111	17	0.4046858868820564
1111	18	0.7633524577160742
1111	19	1.398196844785204
1111	20	0.7041845939183133
1111	21	0.8038335589744321
1111	22	1.179949042295334
1111	23	0.9476792283082958
1111	24	1.3010542590879268
1111	25	1.2324799678688905
1112	1	0.1350789073190839
1112	2	0.06555208021764614
1112	3	-1.618937959974236
1112	4	-0.34498387467888847
1112	5	-0.23457085681961942
1112	6	0.9635635192524461
1112	7	0.7328402804364664
1112	8	1.014167639262638
1112	9	-0.08343342987819824
1112	10	0.559615423278494
1112	11	0.179667520096487
1112	12	0.652825782947609
1112	13	0.08137726320290284
1112	14	0.2637510380972188
1112	15	0.7564183807639608
1112	16	0.5623420932689243
1112	17	-0.32839045824475477
1112	18	0.0317686296973676
1112	19	0.8313483234263046
1112	20	0.6214869623162116
1112	21	0.5204098623604025
1112	22	0.46973088435250077
1112	23	0.5230349843703053
1112	24	1.9147213386504802
1112	25	0.9455398874105967
1113	1	-0.14655218938203518
1113	2	0.10599557101548385
1113	3	-4.128513534587682
1113	4	-1.0614992589992234
1113	5	0.28473484633305546
1113	6	1.0869765211860631
1113	7	1.0347630666489032
1113	8	1.248248020841615
1113	9	1.041247591125341
1113	10	0.9849771584339848
1113	11	0.5797704056295601
1113	12	0.5844170566298956
1113	13	0.14399867828510793
1113	14	-0.27252739362040757
1113	15	0.4642482857076544
1113	16	0.796819576905385
1113	17	0.7380192202153921
1113	18	0.5011443897755257
1113	19	1.469322110177984
1113	20	0.7530168656805216
1113	21	0.8282496948555362
1113	22	0.8837706983462894
1113	23	0.7035178694972544
1113	24	2.1099714565401513
1113	25	1.5392740230705897
1114	1	0.5665118928929971
1114	2	0.24032246516790323
1114	3	0.4922875919420022
1114	4	-0.04580239314083201
1114	5	0.6475000756280593
1114	6	1.11388854811117
1114	7	1.1687455496354282
1114	8	1.112342938815079
1114	9	0.2695791152101492
1114	10	0.48240279395977836
1114	11	0.3404460618636236
1114	12	-0.22864615371813102
1114	13	0.12311166694569664
1114	14	0.1438831444893438
1114	15	-2.4010760493390144
1114	16	-0.17383136019815737
1114	17	0.1812720310900673
1114	18	0.3387654241425295
1114	19	0.8704172504928422
1114	20	0.8932924818059362
1114	21	0.4665176990582225
1114	22	0.6810672886757487
1114	23	0.3479743755985609
1114	24	1.4122009738849641
1114	25	1.0439170343576833
1115	1	-3.254143373755351
1115	2	-0.894635331107434
1115	3	-0.12174252297416288
1115	4	0.4667548647963038
1115	5	1.0472919239586247
1115	6	1.5073363143754506
1115	7	1.0197634805385931
1115	8	1.4367490466168746
1115	9	0.7591336663100847
1115	10	-0.4977860138850687
1115	11	0.40640655632120115
1115	12	0.19073077606638122
1115	13	0.4047860978283069
1115	14	0.3803008153111094
1115	15	1.1886693435202105
1115	16	0.6982981698594202
1115	17	0.5372189589055765
1115	18	0.5346303029839774
1115	19	-1.477639603907015
1115	20	-0.047417830356657475
1115	21	0.04715483357569639
1115	22	0.3350670679743928
1115	23	0.10061035035205634
1115	24	-1.265041687254076
1115	25	-0.41127734511449177
1116	1	1.338585814864249
1116	2	0.23774389186367273
1116	3	0.40279947533366567
1116	4	0.43337315216930605
1116	5	1.3109402970668071
1116	6	1.699743416190671
1116	7	1.1797443055491392
1116	8	-0.8594714039070155
1116	9	0.47824768459701517
1116	10	0.31850411165809334
1116	11	0.8858100109120457
1116	12	1.0619104134646804
1116	13	0.8338529960086731
1116	14	0.9259463475164722
1116	15	0.31940057776656516
1116	16	0.8457920561747088
1116	17	0.9391702570832905
1116	18	0.8126654409312337
1116	19	1.4441648774735825
1116	20	1.3067230174518087
1116	21	1.2885509406993805
1116	22	1.3736423750945406
1116	23	1.3733425433478885
1116	24	2.340332390722743
1116	25	1.525066329016724
1201	1	-0.5375505487721135
1201	2	-0.41849777003836763
1201	3	0.08814348143238959
1201	4	-0.3422486317071929
1201	5	-0.5304166688184573
1201	6	0.04474770435533415
1201	7	0.24751398346032616
1201	8	0.0951598139317511
1201	9	-0.5714116445434456
1201	10	-0.3030391511587567
1201	11	-0.051823398655322706
1201	12	0.08339650075265581
1201	13	-0.062204274812749816
1201	14	-0.2968952762685433
1201	15	0.2685524740882741
1201	16	-0.03032286663832906
1201	17	-0.12388243969581936
1201	18	-0.37421992213337063
1201	19	-0.8939354655795881
1201	20	-0.2148440685020674
1201	21	-0.09219519995242109
1201	22	-0.11271887895539123
1201	23	-0.05526904422596601
1201	24	-1.482394882004531
1201	25	-1.0296315936057283
1202	1	-0.8981347394082668
1202	2	-0.49603293694997674
1202	3	-0.6876280911059247
1202	4	-0.6685267127485552
1202	5	-0.8395915585938099
1202	6	0.4241540658921821
1202	7	-0.15035238227555325
1202	8	0.3088801582525562
1202	9	0.3868735748560894
1202	10	0.40048541319144704
1202	11	0.011920565232976799
1202	12	0.2695423122160179
1202	13	0.17236689881731593
1202	14	0.21071547325906437
1202	15	0.6834590455768321
1202	16	0.3119561591857334
1202	17	-0.02336018481444313
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2211	3	0.34705566383022557
2211	4	0.7921043293593684
2211	5	0.852570482181445
2211	6	-1.6836203277188595
2211	7	0.2610018107233927
2211	8	1.0290454288481818
2211	9	1.0037079091228662
2211	10	0.9963848175399193
2211	11	0.8481053521900037
2211	12	0.9185005969752424
2211	13	0.9496025554451961
2211	14	0.950254757544613
2211	15	1.4726776793824499
2211	16	0.7100572898682884
2211	17	1.0393034843694835
2211	18	0.7448398305475266
2211	19	0.7909657516941024
2211	20	0.6416782481823091
2211	21	0.44070741815211534
2211	22	-2.3729896257431413
2211	23	-0.7713327095723059
2211	24	1.515146418352927
2211	25	1.1176971669005056
2212	1	-0.6890215470989425
2212	2	-0.3888268274839888
2212	3	-3.0818695321389065
2212	4	-1.6859114872565506
2212	5	-0.6416455217651027
2212	6	0.0028649194510776965
2212	7	0.09093515839990829
2212	8	-0.14059962090743738
2212	9	-0.2402511128461029
2212	10	0.06325337533315434
2212	11	0.040798371190369664
2212	12	-0.0018958398310999769
2212	13	-0.15215361483877388
2212	14	-0.38599176260429857
2212	15	-1.3502242502099193
2212	16	-0.14882537639850568
2212	17	-0.09869420768243486
2212	18	0.01911294642955852
2212	19	0.7075634189714393
2212	20	-0.6112908987679191
2212	21	-0.6519321399040834
2212	22	-0.1121262966910308
2212	23	-0.15840624444905416
2212	24	1.040741388677752
2212	25	0.5396191965039563
2213	1	-0.36865764590033834
2213	2	-0.7451302924455376
2213	3	-0.030477334371525444
2213	4	-6.897043308861026
2213	5	-1.7065461731169762
2213	6	-3.4202216572689537
2213	7	-1.4635409827002732
2213	8	-3.0917708610270935
2213	9	-1.4156017853885174
2213	10	-0.6077789536098237
2213	11	-0.602488754426318
2213	12	-0.4763231809740702
2213	13	-0.6566139144688705
2213	14	-0.6721010564039034
2213	15	-3.185114985212987
2213	16	-1.5135906551878122
2213	17	-0.819769123434618
2213	18	-0.6110339883488507
2213	19	0.09742405518134234
2213	20	-1.9863983944578045
2213	21	-0.7087536415108602
2213	22	-0.7733760035209656
2213	23	-0.6200349121608468
2213	24	-0.5691243497199139
2213	25	-0.2478499949227171
2214	1	0.867731543507464
2214	2	0.6206068439406174
2214	3	0.3287099501395616
2214	4	0.7658658041082447
2214	5	0.8951201059645477
2214	6	0.5321791799149516
2214	7	1.1109390634055067
2214	8	-2.129349094245498
2214	9	-0.6570272224328946
2214	10	-0.42701699492302936
2214	11	0.3187967475301505
2214	12	-0.9510084786900137
2214	13	-0.13527988011197323
2214	14	0.13569951587398776
2214	15	-1.1884141773507366
2214	16	0.034101528062443265
2214	17	0.20339599794694152
2214	18	0.31539291477845666
2214	19	1.2117006696419708
2214	20	-0.7708114051854906
2214	21	0.06633609287536046
2214	22	0.39473315393634323
2214	23	-0.15354451247630863
2214	24	-1.7355470062621805
2214	25	-0.5699696475116198
2215	1	0.5993898349647502
2215	2	-0.3130614977679258
2215	3	0.21614713606921043
2215	4	0.6010416835052332
2215	5	0.9483864376228404
2215	6	1.0899681246385704
2215	7	0.5762656965713369
2215	8	-0.757489797763089
2215	9	0.13191750118519963
2215	10	-0.3677205125109708
2215	11	-0.1254243579974883
2215	12	-0.4243753128608674
2215	13	-0.03678741780041861
2215	14	-0.11455174040742122
2215	15	-0.055750860767822985
2215	16	0.1486743923840521
2215	17	-0.5363024945153754
2215	18	-0.7619940198949067
2215	19	0.26898710180277874
2215	20	-3.4753340182508126
2215	21	-2.1738649121497673
2215	22	-1.3017492581239622
2215	23	-1.1022882310692736
2215	24	-0.8762505238501213
2215	25	-0.7830349741447868
2216	1	0.1132551943149096
2216	2	-0.7542787743849857
2216	3	-0.21593494268672586
2216	4	-0.31061425641819085
2216	5	-0.17576023674595476
2216	6	-1.4039725860358594
2216	7	-9.422527864315498
2216	8	-10.64386265901748
2216	9	-5.412141552986984
2216	10	-2.9907287377674656
2216	11	-2.1075445213779744
2216	12	-1.400605395113434
2216	13	-1.2751296886664463
2216	14	-1.3937462889384804
2216	15	-2.303009534604964
2216	16	-1.3859110475316996
2216	17	-1.6697612429881517
2216	18	-1.3981575435150404
2216	19	-0.6907321455019044
2216	20	-1.471240304514275
2216	21	-3.796604244620873
2216	22	-2.8323330008457077
2216	23	-2.961578579045163
2216	24	-2.8097184739771563
2216	25	-2.1180436901561777
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40038.0
3	6.0
4	324.0
5	1738.0
6	2132.0
7	1795.0
8	1536.0
9	1341.0
10	1264.0
11	1210.0
12	1231.0
13	1194.0
14	1225.0
15	1234.0
16	1325.0
17	1481.0
18	1647.0
19	2057.0
20	2648.0
21	3322.0
22	4218.0
23	4998.0
24	5821.0
25	6437.0
26	7376.0
27	8261.0
28	9301.0
29	10902.0
30	12909.0
31	15605.0
32	19921.0
33	26280.0
34	35133.0
35	49466.0
36	70014.0
37	110808.0
38	130316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.61741809543969	18.932208458067485	12.657702606901788	25.792670839591043
2	13.93910297837768	20.312156407213372	42.57386353144981	23.174877082959142
3	18.523156673711565	24.98644458731952	29.919103983025096	26.571294755943818
4	11.268338100470562	16.87398820657781	39.612645635343355	32.245028057608266
5	12.54347046020839	38.31403362274515	36.59449815722138	12.547997759825078
6	31.191219153941468	37.903616599233054	18.94602348467192	11.959140762153561
7	27.18154885008188	31.374151306962055	23.643688481314463	17.800611361641597
8	24.016631023954595	37.747145220514064	21.417739310548896	16.81848444498245
9	25.93103055118216	15.67431074983778	20.775340412368024	37.61931828661204
10	13.721031244916476	29.908316269459945	35.76135062025293	20.60930186537065
11	33.67201856397462	23.07586549166365	25.079808994943193	18.172306949418534
12	22.6659825826853	25.425624310466823	32.63399159649776	19.274401510350124
13	28.328624722303726	22.889885568177053	26.118791130485814	22.662698579033407
14	19.541794793549446	23.03718557914469	28.707743278997476	28.71327634830839
15	22.342307298940863	32.3599288431649	23.423240653958814	21.87452320393542
16	20.82773722872516	29.17402334351112	29.146692555462216	20.851546872301505
17	20.586647350965617	29.57062806209193	28.672917442145117	21.169807144797335
18	20.252470549573626	28.61880602119961	30.73323429897422	20.395489130252535
19	22.987599875914917	27.6610800432621	28.01941763852674	21.331902442296244
20	22.59601116672116	28.385939320774963	28.96556927642665	20.052480236077226
21	22.072981392494626	27.546418164198073	27.93305456167236	22.44754588163494
22	21.87457034878457	29.207060867115796	28.379783170750517	20.538585613349117
23	21.003972093070047	28.879581362516788	28.732702783818542	21.383743760594623
24	22.390502106712137	27.78965405657356	29.138016890583422	20.681826946130876
25	21.509769928003998	28.274778596362975	29.026092263531527	21.1893592121015
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	153.0
1	153.0
2	230.5
3	308.0
4	308.0
5	308.0
6	489.0
7	670.0
8	670.0
9	670.0
10	1030.0
11	1390.0
12	1390.0
13	1390.0
14	2321.5
15	3253.0
16	3253.0
17	3253.0
18	5634.0
19	8015.0
20	8015.0
21	8015.0
22	12567.5
23	17120.0
24	17120.0
25	17120.0
26	24449.0
27	31778.0
28	31778.0
29	31778.0
30	41026.0
31	50274.0
32	50274.0
33	50274.0
34	57310.0
35	64346.0
36	64346.0
37	64346.0
38	69148.5
39	73951.0
40	73951.0
41	73951.0
42	76461.5
43	78972.0
44	78972.0
45	78972.0
46	78464.0
47	77956.0
48	77956.0
49	77956.0
50	73047.0
51	68138.0
52	68138.0
53	68138.0
54	58949.0
55	49760.0
56	49760.0
57	49760.0
58	41529.0
59	33298.0
60	33298.0
61	33298.0
62	26559.5
63	19821.0
64	19821.0
65	19821.0
66	14896.5
67	9972.0
68	9972.0
69	9972.0
70	7261.5
71	4551.0
72	4551.0
73	4551.0
74	3163.5
75	1776.0
76	1776.0
77	1776.0
78	1226.0
79	676.0
80	676.0
81	676.0
82	447.5
83	219.0
84	219.0
85	219.0
86	148.0
87	77.0
88	77.0
89	77.0
90	54.0
91	31.0
92	31.0
93	31.0
94	17.5
95	4.0
96	4.0
97	4.0
98	4.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20921554230076744
2	0.1319331985502436
3	0.13595657436371988
4	0.07024143607694036
5	0.022128566974119634
6	0.020955082361855715
7	0.29772981019724604
8	0.28867721461692436
9	0.0176022691839588
10	0.03771914825134028
11	0.015087659300536113
12	0.01609350325390519
13	0.014920018641641268
14	0.01676406588948457
15	0.014249456006061888
16	0.01961395709069695
17	0.01810519116064334
18	0.014752377982746423
19	0.02497845817533201
20	0.016596425230589726
21	0.014752377982746423
22	0.01676406588948457
23	0.01726698786616911
24	0.014584737323851578
25	0.015422940618325807
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	596514.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.34435822994267
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.72857474921518	83.68735142256567
2	4.117703731566069	7.27551787092489
3	0.5450897188110787	1.4446680415831394
4	0.20690814195770602	0.7311666805521364
5	0.10138818363142188	0.44785370075087716
6	0.06400680577540127	0.33927841071458475
7	0.038280182781018794	0.23672867264998054
8	0.02487069148737448	0.17577482225495974
9	0.024221878880030565	0.19258797103017325
>10	0.1261772941179589	2.0883147008994984
>50	0.011958831597710672	0.7190861829534493
>100	0.009301223136600556	1.5498467879036586
>500	0.0013287461621538255	0.9224959077569012
>1k	1.8982088030768937E-4	0.18932882746001478
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	1129	0.18926630389228083	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1000	0.16764065889484572	No Hit
CTGTAGGACGTGGAATATGGCAAGA	988	0.16562897098810758	No Hit
GTATCAACGCAGAGTACTTTTTTTT	910	0.1525529995943096	No Hit
GTCCTACAGTGTGCATTTCTCATTT	821	0.13763298095266835	No Hit
CTTTAGGACGTGAAATATGGCGAGG	731	0.12254532165213222	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.676406588948457E-4	0.0
10	0.0	0.0	0.0	3.352813177896914E-4	0.0
11	0.0	0.0	0.0	3.352813177896914E-4	0.0
12	0.0	0.0	0.0	3.352813177896914E-4	0.0
13	0.0	0.0	0.0	5.029219766845372E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCAACG	30	7.804738E-4	18.967394	18
GAGTAGG	45	6.551924E-4	14.836987	1
TTAACCT	40	0.005196579	14.28181	4
GAGCACC	40	0.005334495	14.225546	11
GCAAGAC	75	9.297746E-7	13.978554	3
TAGGACC	315	0.0	13.903984	4
TAGGACT	135	2.7284841E-11	12.6949415	4
CCTACAC	100	1.3772114E-7	12.39008	3
CTAGGAC	100	1.3772114E-7	12.39008	3
GTCCTAA	210	0.0	12.263224	1
GTCCTAC	695	0.0	12.076972	1
GTATTAT	80	2.7447091E-5	11.922579	1
TAGGGCT	65	7.862173E-4	11.718409	4
TCCTACA	725	0.0	11.569432	2
GGCGAGG	185	0.0	11.277908	19
CTGCGTT	135	4.825779E-9	11.239936	11
CCTACAG	730	0.0	11.228103	3
GCGTTGA	110	5.059046E-7	11.208005	13
CTACACC	85	5.1905627E-5	11.20142	4
GTAGGAC	760	0.0	11.161104	3
>>END_MODULE