FastQCFastQC Report
Thu 26 May 2016
SRR1926971_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926971_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596514
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15930.2670515696194892No Hit
CTGTAGGACGTGGAATATGGCAAGA11910.19966002474376124No Hit
GTCCTACAGTGGACATTTCTAAATT11770.1973130555192334No Hit
CTTTAGGACGTGAAATATGGCGAGG9640.16160559517463127No Hit
GTCCTAAAGTGTGTATTTCTCATTT9470.1587557039734189No Hit
GGTATCAACGCAGAGTACTTTTTTT9420.15791750067894467No Hit
GTCCTACAGTGTGCATTTCTCATTT8730.1463502952152003No Hit
TATCAACGCAGAGTACTTTTTTTTT8350.1399799501771962No Hit
CTGTAGGACCTGGAATATGGCGAGA6740.11298980409512602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGGG250.00594445519.045856
GGCTTAT551.8982602E-413.8678631
TGTAGGA6350.012.4598692
GTCCTAT701.05283885E-412.2582011
ATTACAC550.003009783812.1200883
CTGTAGG6550.012.08144951
TAGGACC2150.011.9590234
GTCCTAC7450.011.9016981
GCTTTAG657.773008E-411.7343451
ACGTGAA3000.011.7016258
GGACGTG5600.011.5635536
GTCCTAA2150.011.5296761
TCCTACA8200.011.5088112
AGGACGT5550.011.4961455
GTAGGAC6650.011.4561513
CCTACAG8200.011.3810583
TAGGACA2350.011.3464654
GACGTGA3050.011.1996587
TAGGACG6000.011.1100814
TAAGGGT600.005764072811.110084
Produced by FastQC (version 0.11.5)