##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926971_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 596514 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.766785020971845 31.0 30.0 33.0 25.0 34.0 2 30.073277408409528 31.0 30.0 33.0 26.0 34.0 3 30.107065383209783 31.0 30.0 34.0 26.0 34.0 4 33.693772484803375 35.0 35.0 37.0 28.0 37.0 5 33.71601840023872 37.0 35.0 37.0 28.0 37.0 6 33.66112614289019 37.0 35.0 37.0 29.0 37.0 7 33.65449930764408 37.0 35.0 37.0 28.0 37.0 8 33.68593360759345 37.0 35.0 37.0 29.0 37.0 9 35.192833697113564 39.0 35.0 39.0 29.0 39.0 10 35.077106321058686 39.0 35.0 39.0 29.0 39.0 11 35.12496102354681 39.0 35.0 39.0 29.0 39.0 12 35.0238602949805 39.0 35.0 39.0 28.0 39.0 13 35.029610369580595 39.0 35.0 39.0 28.0 39.0 14 36.12137351344646 40.0 36.0 41.0 28.0 41.0 15 36.11073168441981 40.0 36.0 41.0 28.0 41.0 16 36.08789232105198 40.0 36.0 41.0 28.0 41.0 17 36.06641252342778 40.0 36.0 41.0 28.0 41.0 18 36.04384641433395 40.0 36.0 41.0 28.0 41.0 19 36.10618024053082 40.0 36.0 41.0 27.0 41.0 20 36.068102341269444 40.0 36.0 41.0 27.0 41.0 21 36.01751844885451 40.0 36.0 41.0 27.0 41.0 22 35.93018101838347 40.0 36.0 41.0 27.0 41.0 23 35.85637554189843 40.0 36.0 41.0 27.0 41.0 24 35.78960929668038 40.0 36.0 41.0 27.0 41.0 25 35.66888958180361 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.20646851847899583 1101 2 -0.133239824490758 1101 3 -0.07566764769779866 1101 4 -0.1708315478590876 1101 5 -0.178825184982081 1101 6 -0.2310164580438041 1101 7 -0.39365189685574364 1101 8 -0.3817952012121566 1101 9 -0.3050940989842843 1101 10 -0.3003775186741393 1101 11 -0.3427257848941423 1101 12 -0.1515424760551909 1101 13 -0.3945706320701632 1101 14 -0.2889290966193556 1101 15 -0.4062034220194377 1101 16 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1104 12 0.02225123290800468 1104 13 0.03450632624408456 1104 14 -0.02494222839519722 1104 15 -0.07759218366243914 1104 16 0.05360992369926265 1104 17 0.12531684800360665 1104 18 -0.008587957151632963 1104 19 0.07737860842031807 1104 20 0.19031401747618304 1104 21 0.056634016700137124 1104 22 0.017393361102989502 1104 23 -0.13000318164169045 1104 24 0.16359829460744635 1104 25 -0.01876806717780255 1105 1 0.01068506463132124 1105 2 -0.16260348788653545 1105 3 0.05221296485052207 1105 4 0.0602233138715107 1105 5 -0.1115489435309911 1105 6 -0.14291803401764014 1105 7 -0.5384407758328464 1105 8 -0.5514502710902462 1105 9 -0.6675494959512775 1105 10 -0.4556406765688763 1105 11 -0.2716768702346144 1105 12 -0.2491747983864414 1105 13 -0.17836114649180956 1105 14 -0.18390382156731988 1105 15 -0.2809172192243139 1105 16 -0.29563734989011436 1105 17 -0.23219856214338108 1105 18 -0.009980797360256588 1105 19 -0.15750759357114674 1105 20 -0.2764652618030965 1105 21 -0.11026972915097843 1105 22 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2212 20 0.4866993598737963 2212 21 0.38351457824327895 2212 22 0.6375710541334456 2212 23 0.48932473523224473 2212 24 0.5224318213857586 2212 25 0.5363706605069325 2213 1 -0.1535657913819506 2213 2 -0.09005415681237139 2213 3 -0.013839048986888258 2213 4 -0.08193886975656994 2213 5 -0.16385400195169098 2213 6 -0.24414380972451255 2213 7 -0.07418594862397754 2213 8 -0.11843992833193795 2213 9 -0.27606682801383187 2213 10 -0.35188657300009396 2213 11 -0.20147716322060205 2213 12 -0.16266230235763857 2213 13 -0.3210572353053891 2213 14 -0.47074769180629517 2213 15 -0.4642386389708619 2213 16 -0.4901707785321463 2213 17 -0.35589248003670804 2213 18 -0.4538700098280515 2213 19 -0.40253918663832167 2213 20 -0.34852260586747974 2213 21 -0.39788136387158346 2213 22 -0.33775328453661047 2213 23 -0.48338473220633915 2213 24 -0.310786301256023 2213 25 -0.3903964863414373 2214 1 0.20257102954360207 2214 2 0.22050062919007374 2214 3 0.2332838896499574 2214 4 0.3516111742259156 2214 5 0.32804816481776555 2214 6 0.17955617915347943 2214 7 0.07698944544900144 2214 8 0.09906105748244443 2214 9 0.06771344154321213 2214 10 -0.09937249354850763 2214 11 -0.09788576558857898 2214 12 -0.02786760452238468 2214 13 -0.14088979852390082 2214 14 0.08937485250479682 2214 15 0.07277338290916191 2214 16 0.009726090116934927 2214 17 -0.055117983813133264 2214 18 0.039991652608883044 2214 19 0.060036266077979406 2214 20 -0.23167882964229136 2214 21 0.07182936280000973 2214 22 0.0827324439043835 2214 23 0.2086812314145874 2214 24 0.08972328894851955 2214 25 0.048524476622276325 2215 1 0.48096298934259707 2215 2 0.3873348000443464 2215 3 0.3368014775896562 2215 4 0.582766953120391 2215 5 0.4968923859232959 2215 6 0.40970693292232596 2215 7 0.36442663137864884 2215 8 0.41463894692967784 2215 9 0.2445978636537589 2215 10 0.2564064009238507 2215 11 0.12723986255212338 2215 12 0.1369565160806303 2215 13 0.07016547785801208 2215 14 0.15369646054499952 2215 15 0.27880353366293065 2215 16 0.2288215675038714 2215 17 0.1489021166893778 2215 18 0.17064491894053901 2215 19 0.2741066178367717 2215 20 0.22962770302102342 2215 21 0.1874072522472403 2215 22 0.2656470167687104 2215 23 0.25993751282162236 2215 24 0.2073112286470149 2215 25 0.06862497913483878 2216 1 -0.18400068968325556 2216 2 0.07065577930483968 2216 3 -1.8548976078681498 2216 4 -1.3283327979237427 2216 5 -0.5869152586065809 2216 6 -0.16723269911374672 2216 7 -0.14296996503168202 2216 8 -0.21157145400684385 2216 9 -0.08957145700855307 2216 10 -0.3160438462428061 2216 11 -0.21552380318244957 2216 12 -0.00938454834628999 2216 13 0.021839412127526714 2216 14 -0.22409585811851684 2216 15 -0.2084874482046999 2216 16 -0.2779339803268428 2216 17 -0.15342743544565707 2216 18 -0.05236023909787235 2216 19 -0.4451519333116494 2216 20 -0.376859463227305 2216 21 -0.31342958708675894 2216 22 -0.3684921429157768 2216 23 -0.6936207157576959 2216 24 -0.6989627659444579 2216 25 -0.6375886935009731 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15270.0 3 11.0 4 250.0 5 1643.0 6 2202.0 7 1669.0 8 1341.0 9 1114.0 10 941.0 11 860.0 12 821.0 13 782.0 14 747.0 15 768.0 16 832.0 17 835.0 18 972.0 19 1119.0 20 1434.0 21 1859.0 22 2330.0 23 2837.0 24 3457.0 25 4053.0 26 4833.0 27 5296.0 28 6062.0 29 7566.0 30 9628.0 31 12547.0 32 15977.0 33 21203.0 34 28549.0 35 40326.0 36 58240.0 37 101698.0 38 236442.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.22971013519864 19.988621564652338 10.290603224896957 28.49106507525207 2 13.900482133537398 21.39175993005456 40.46464717659043 24.24311075981762 3 18.22149490871228 25.433803199471505 29.33513969393589 27.00956219788032 4 11.683045159040693 17.93118015671049 38.68274675866786 31.703027925580958 5 12.320575365890459 39.082298110613756 35.914432765008634 12.682693758487149 6 30.658699959964608 39.00372430081047 18.118033757573922 12.219541981651005 7 27.6508590696528 32.148671782911165 22.23738726125543 17.963081886180607 8 24.346337562264544 38.80529200415125 20.202800919444645 16.645569514139563 9 26.030389857305487 15.866402659741235 20.268120157800585 37.83508732515269 10 13.973551679508587 30.540299488334977 34.81015417010347 20.67599466205296 11 33.72881753743604 23.334741585692157 24.782726778924495 18.153714097947304 12 22.859968818628353 25.69378551910278 32.154364553821395 19.29188110844747 13 28.564674957167234 23.040505879236754 25.827233925559845 22.567585238036163 14 19.790985626489906 23.416885437726524 28.15139292623476 28.64073600954881 15 21.869048577023147 32.5824481639441 23.544499879295085 22.004003379737668 16 20.956369256973105 29.456032294729646 28.68437199290535 20.9032264553919 17 20.57541076221306 29.88853553904106 28.39217250923283 21.143881189513053 18 20.36737143508553 28.775473254340262 30.528509257210295 20.32864605336391 19 22.429259477193213 27.96042319420765 28.197300636534784 21.413016692064353 20 22.522619181334 28.411066425037763 28.932267349754486 20.13404704387375 21 21.740508186139312 27.624468718301237 28.031218825186315 22.603804270373136 22 21.98979737239591 29.281172413677485 27.95127683721897 20.777753376707633 23 20.908848591732283 28.962486986872037 28.674647493013566 21.454016928382114 24 21.93531406691891 27.838286843706676 29.39298892061028 20.833410168764136 25 21.57449847606466 28.532126702901344 28.810257476336254 21.083117344697744 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 219.0 1 219.0 2 210.0 3 201.0 4 201.0 5 201.0 6 402.5 7 604.0 8 604.0 9 604.0 10 968.0 11 1332.0 12 1332.0 13 1332.0 14 2175.0 15 3018.0 16 3018.0 17 3018.0 18 5340.0 19 7662.0 20 7662.0 21 7662.0 22 12152.0 23 16642.0 24 16642.0 25 16642.0 26 23819.0 27 30996.0 28 30996.0 29 30996.0 30 40370.0 31 49744.0 32 49744.0 33 49744.0 34 56764.0 35 63784.0 36 63784.0 37 63784.0 38 68284.5 39 72785.0 40 72785.0 41 72785.0 42 75769.0 43 78753.0 44 78753.0 45 78753.0 46 79149.0 47 79545.0 48 79545.0 49 79545.0 50 74974.5 51 70404.0 52 70404.0 53 70404.0 54 60387.5 55 50371.0 56 50371.0 57 50371.0 58 41973.5 59 33576.0 60 33576.0 61 33576.0 62 26656.0 63 19736.0 64 19736.0 65 19736.0 66 14949.0 67 10162.0 68 10162.0 69 10162.0 70 7326.0 71 4490.0 72 4490.0 73 4490.0 74 3084.5 75 1679.0 76 1679.0 77 1679.0 78 1130.0 79 581.0 80 581.0 81 581.0 82 373.5 83 166.0 84 166.0 85 166.0 86 109.5 87 53.0 88 53.0 89 53.0 90 31.0 91 9.0 92 9.0 93 9.0 94 5.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10896642828164972 2 0.1041048491736992 3 0.01726698786616911 4 0.0 5 0.004023375813476298 6 0.34198694414548525 7 0.018943394455117567 8 0.011231924145954662 9 0.010896642828164972 10 0.005029219766845372 11 0.005029219766845372 12 6.705626355793829E-4 13 0.0013411252711587659 14 0.0 15 0.003017531860107223 16 0.0013411252711587659 17 1.6764065889484574E-4 18 0.0010058439533690744 19 5.029219766845372E-4 20 0.0011734846122639201 21 0.01324361205269281 22 1.6764065889484574E-4 23 1.6764065889484574E-4 24 1.6764065889484574E-4 25 0.0053645010846350635 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 596514.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.69940447747011 #Duplication Level Percentage of deduplicated Percentage of total 1 93.21788467717712 79.88717203483566 2 5.303005681488167 9.08928857688353 3 0.7628884273505481 1.9613725172008707 4 0.23749242596767128 0.8141183789335636 5 0.12417331618937158 0.532078962471087 6 0.07015244555788933 0.36072136841695557 7 0.05600984845668336 0.33600074603277785 8 0.03501198932971739 0.2400405308102655 9 0.02411783020864704 0.1860195317552811 >10 0.1434237564291324 2.338286147815104 >50 0.012919835676930672 0.7758934872553236 >100 0.010570717774017462 1.6762583235920732 >500 0.001761786295585024 1.1382518740250762 >1k 5.872620985283413E-4 0.6644975199724874 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1593 0.2670515696194892 No Hit CTGTAGGACGTGGAATATGGCAAGA 1191 0.19966002474376124 No Hit GTCCTACAGTGGACATTTCTAAATT 1177 0.1973130555192334 No Hit CTTTAGGACGTGAAATATGGCGAGG 964 0.16160559517463127 No Hit GTCCTAAAGTGTGTATTTCTCATTT 947 0.1587557039734189 No Hit GGTATCAACGCAGAGTACTTTTTTT 942 0.15791750067894467 No Hit GTCCTACAGTGTGCATTTCTCATTT 873 0.1463502952152003 No Hit TATCAACGCAGAGTACTTTTTTTTT 835 0.1399799501771962 No Hit CTGTAGGACCTGGAATATGGCGAGA 674 0.11298980409512602 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.676406588948457E-4 0.0 10 0.0 0.0 0.0 1.676406588948457E-4 0.0 11 0.0 0.0 0.0 1.676406588948457E-4 0.0 12 0.0 0.0 0.0 1.676406588948457E-4 0.0 13 0.0 0.0 0.0 3.352813177896914E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACGGG 25 0.005944455 19.04585 6 GGCTTAT 55 1.8982602E-4 13.867863 1 TGTAGGA 635 0.0 12.459869 2 GTCCTAT 70 1.05283885E-4 12.258201 1 ATTACAC 55 0.0030097838 12.120088 3 CTGTAGG 655 0.0 12.0814495 1 TAGGACC 215 0.0 11.959023 4 GTCCTAC 745 0.0 11.901698 1 GCTTTAG 65 7.773008E-4 11.734345 1 ACGTGAA 300 0.0 11.701625 8 GGACGTG 560 0.0 11.563553 6 GTCCTAA 215 0.0 11.529676 1 TCCTACA 820 0.0 11.508811 2 AGGACGT 555 0.0 11.496145 5 GTAGGAC 665 0.0 11.456151 3 CCTACAG 820 0.0 11.381058 3 TAGGACA 235 0.0 11.346465 4 GACGTGA 305 0.0 11.199658 7 TAGGACG 600 0.0 11.110081 4 TAAGGGT 60 0.0057640728 11.11008 4 >>END_MODULE