FastQCFastQC Report
Thu 26 May 2016
SRR1926970_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926970_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672683
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT24740.3677809607199825No Hit
CTGTAGGACGTGGAATATGGCAAGA23990.35663157832143816No Hit
GTATCAACGCAGAGTACTTTTTTTT19110.2840862635149097No Hit
GTCCTACAGTGTGCATTTCTCATTT17020.25301665123096617No Hit
GTCCTAAAGTGTGTATTTCTCATTT16560.24617836335985893No Hit
CTTTAGGACGTGAAATATGGCGAGG15770.23443434723339224No Hit
CTGTAGGACCTGGAATATGGCGAGA14260.211986924004323No Hit
GGTATCAACGCAGAGTACTTTTTTT11510.17110585520966043No Hit
TATCAACGCAGAGTACTTTTTTTTT9830.14613123863692112No Hit
GTACATGGGAAGCAGTGGTATCAAC8560.12725161777538602No Hit
CTGAAGGACCTGGAATATGGCGAGA8460.12576503345558013No Hit
CACACACACACACACACACACACAC7030.10450687768235559No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCGT402.7023637E-416.6696136
AGGCGTG402.7818594E-416.606397
ATTAGGC400.00518199814.28823953
TCCAACG602.6003096E-514.22875718
CCAGACT551.9135352E-413.8552634
TAGGACC3950.013.7456474
GGACGTG9450.012.9022546
AGGACGT9950.012.54110055
GACGTGA4250.012.5036347
TGTAGGA12250.012.2987952
AGGACAC701.0622053E-412.2470625
TAGGACG10200.012.2337214
GGACCTG7450.012.1466026
GTCCTAA5150.012.0377431
GACGTGG5650.011.9246897
CTGTAGG12000.011.9219951
TAAGAGT802.78327E-511.9068664
GCCATAT3450.011.8874381
GTAGGAC11700.011.8865123
AGGACCT7850.011.7703545