##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926970_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 672683 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.77397526026375 31.0 30.0 33.0 25.0 34.0 2 30.09565426805791 31.0 30.0 34.0 26.0 34.0 3 30.166371084151077 31.0 30.0 34.0 26.0 34.0 4 33.69857719014751 35.0 35.0 37.0 28.0 37.0 5 33.64618549896459 37.0 35.0 37.0 28.0 37.0 6 33.58704322838543 37.0 35.0 37.0 28.0 37.0 7 33.57614359215262 37.0 35.0 37.0 28.0 37.0 8 33.60125348789846 37.0 35.0 37.0 28.0 37.0 9 35.11407304778031 39.0 35.0 39.0 29.0 39.0 10 35.011351557866035 39.0 35.0 39.0 28.0 39.0 11 35.03103542084459 39.0 35.0 39.0 28.0 39.0 12 34.92870044285347 39.0 35.0 39.0 27.0 39.0 13 34.94953492209555 39.0 35.0 39.0 27.0 39.0 14 36.03019401411958 40.0 36.0 41.0 27.0 41.0 15 36.03058201262705 40.0 36.0 41.0 27.0 41.0 16 36.01508437109307 40.0 36.0 41.0 27.0 41.0 17 35.99911994208267 40.0 36.0 41.0 27.0 41.0 18 35.97056117071488 40.0 36.0 41.0 27.0 41.0 19 36.038624433797196 40.0 36.0 41.0 27.0 41.0 20 35.99829042803222 40.0 36.0 41.0 27.0 41.0 21 35.941440470474205 40.0 36.0 41.0 27.0 41.0 22 35.860283372703044 40.0 36.0 41.0 27.0 41.0 23 35.78728464967897 40.0 36.0 41.0 27.0 41.0 24 35.72129368513847 40.0 36.0 41.0 27.0 41.0 25 35.59658858630291 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.030382401115140567 1101 2 0.13101660511801683 1101 3 0.4598480877024329 1101 4 0.12494181050149678 1101 5 0.13796706111176604 1101 6 0.033685184800333445 1101 7 0.17948046740706758 1101 8 -0.024645218918557532 1101 9 0.1311724928990472 1101 10 0.11403508831598685 1101 11 0.1954843119372427 1101 12 0.3171048494970563 1101 13 0.12150771907852231 1101 14 0.37532390639398727 1101 15 0.04037636248304466 1101 16 0.06166977439352905 1101 17 0.2488608666236587 1101 18 0.0835060772770504 1101 19 0.1466811914476267 1101 20 0.18567470318734536 1101 21 0.2195765074286129 1101 22 -0.07730841008690703 1101 23 0.1949032114300806 1101 24 0.2172795978609514 1101 25 0.17523787273120917 1102 1 -0.45485182504650723 1102 2 -0.2735310877361279 1102 3 -0.12754878497669608 1102 4 -0.4508133801730523 1102 5 -0.7485262870751299 1102 6 -0.49718943982428954 1102 7 -0.5188014838990753 1102 8 -0.42474693353639736 1102 9 -0.3921855425557652 1102 10 -0.3889517695095748 1102 11 -0.29056827290566645 1102 12 -0.1579437878499732 1102 13 -0.19351496489577613 1102 14 -0.404989673314752 1102 15 -0.3086238474042631 1102 16 -0.3469757663084607 1102 17 -0.31467847917375025 1102 18 -0.1923292338405247 1102 19 -0.2793607779411218 1102 20 -0.3037693779906121 1102 21 -0.45066429789283546 1102 22 -0.30373140566312173 1102 23 -0.3566987938009589 1102 24 -0.4535918784540698 1102 25 -0.37881315410207606 1103 1 -0.510627013515137 1103 2 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1104 13 0.22121650980409413 1104 14 0.25897853832107387 1104 15 0.2480924695081299 1104 16 0.18580872084245215 1104 17 0.2439862980768268 1104 18 0.19136121770869607 1104 19 0.07586901698293502 1104 20 0.091950179058081 1104 21 0.12160785763370541 1104 22 0.18352399191035573 1104 23 0.37592826412344493 1104 24 0.16200714870893762 1104 25 0.2534582451272982 1105 1 -0.04550464052532632 1105 2 -0.08674127017410527 1105 3 -0.09018727055210007 1105 4 -0.02791946564745018 1105 5 -0.17553136882501974 1105 6 -0.20933474315109635 1105 7 -0.38453952127045454 1105 8 -0.5857689649738091 1105 9 -0.4627697859747357 1105 10 -0.2709036664857791 1105 11 -0.3847818642193417 1105 12 -0.17482837531288453 1105 13 -0.38302714847004893 1105 14 -0.19377944353128385 1105 15 -0.3672572985752751 1105 16 -0.33466001919653365 1105 17 -0.3856493774756231 1105 18 -0.343323883799485 1105 19 -0.32248660648729555 1105 20 -0.07443765966474558 1105 21 -0.3495856096787122 1105 22 -0.3823032287803514 1105 23 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2209 25 0.3343628421028626 2210 1 -0.2295792814086859 2210 2 -0.11118065906777375 2210 3 0.2372028803535926 2210 4 0.021419550191474457 2210 5 -0.02225338878363914 2210 6 -0.14352070177457676 2210 7 0.04218160066269405 2210 8 -0.06242965727660987 2210 9 -0.08611171291288144 2210 10 -0.023596807442103795 2210 11 -0.11744716638620645 2210 12 -0.1903155728373278 2210 13 -0.17614078985106119 2210 14 -0.05456678735904319 2210 15 -0.0832530030201255 2210 16 -0.1459661598198423 2210 17 -0.21119670528860013 2210 18 -0.380636841932791 2210 19 -0.4443075027915455 2210 20 -0.3505393311146463 2210 21 -0.1260521039743736 2210 22 -0.18837837106126187 2210 23 -0.2522571420400297 2210 24 -0.19439594000961335 2210 25 -0.09926703370486223 2211 1 0.011679683527663798 2211 2 0.0737902337652514 2211 3 0.3062000158387477 2211 4 0.2575074616545052 2211 5 0.3660483022041561 2211 6 0.3607334226292451 2211 7 0.3241390665266337 2211 8 0.3572413144549671 2211 9 0.14872164055972092 2211 10 0.30696500708234 2211 11 0.34214398276048286 2211 12 0.3363713278713405 2211 13 0.25025077440150056 2211 14 0.3240058597227815 2211 15 0.5006833237193717 2211 16 0.21793384515574132 2211 17 0.3383974292085483 2211 18 0.17171696474444076 2211 19 0.2879172230182263 2211 20 0.2784384706722989 2211 21 0.1040260126959609 2211 22 0.12132330713217243 2211 23 0.037915368568626207 2211 24 0.10905427216351171 2211 25 0.039368732215230295 2212 1 0.0981038961951235 2212 2 0.07373762802771822 2212 3 0.03957309572933099 2212 4 0.1362757859864132 2212 5 -0.024096889631429974 2212 6 0.03170206960967192 2212 7 -0.09997540355823986 2212 8 -0.1687213705418742 2212 9 -0.11310763628674181 2212 10 -0.019962116435934263 2212 11 -0.02715530881558692 2212 12 -0.16050389131923737 2212 13 0.056097025365936304 2212 14 -0.02705677617537816 2212 15 -0.05198203364894027 2212 16 0.09144860983274583 2212 17 -0.10034494295950935 2212 18 -0.14407527766280026 2212 19 0.003372788705263474 2212 20 -0.1405732428719233 2212 21 -0.19813082254286485 2212 22 -0.06594610438497739 2212 23 -0.01391881814667073 2212 24 -0.17951625459527776 2212 25 -0.1529648583017078 2213 1 0.3182554861027427 2213 2 0.30546517451699273 2213 3 0.43856079499945366 2213 4 0.22778947331308785 2213 5 0.28947474553198305 2213 6 0.07723008226768258 2213 7 0.12415733543830498 2213 8 0.12737063823706762 2213 9 0.08992926590288874 2213 10 0.14893473097036747 2213 11 -0.015380294779482995 2213 12 -0.012496167209462783 2213 13 0.045026368476335676 2213 14 0.09576932927403448 2213 15 0.15449498967651465 2213 16 0.2719494860258109 2213 17 0.12357597848935598 2213 18 0.1422654678669133 2213 19 0.0037853650531545213 2213 20 0.01513987620526791 2213 21 -0.18855479790737917 2213 22 0.06275049462484361 2213 23 0.13716919246972736 2213 24 0.07023534892310579 2213 25 0.14316883344847042 2214 1 0.7438936155097444 2214 2 0.6653937384350783 2214 3 0.5598977840418833 2214 4 0.525855091273435 2214 5 0.2975312189091426 2214 6 0.6316754011651113 2214 7 0.32719974145143027 2214 8 0.3668009976160036 2214 9 0.28333879347524515 2214 10 0.15316609211130583 2214 11 0.12933512387992607 2214 12 0.1772786275153564 2214 13 0.16527763740673862 2214 14 0.16803623002503798 2214 15 0.13397703028865493 2214 16 0.43636160403235635 2214 17 0.31121916131410643 2214 18 0.21419860726286544 2214 19 0.42675101392027415 2214 20 0.3933090159453272 2214 21 0.3376848985052021 2214 22 0.3879041522988018 2214 23 0.3933083303298943 2214 24 0.22486403742419014 2214 25 0.21035602975749867 2215 1 0.57537858463375 2215 2 0.4535396558732572 2215 3 0.19175941855835532 2215 4 0.34891248302098177 2215 5 0.48416550229701727 2215 6 0.5374686516444882 2215 7 0.3909399479958324 2215 8 0.5076231972548726 2215 9 0.4873254796588924 2215 10 0.49535044060759503 2215 11 0.3421930259282817 2215 12 0.24363560188532318 2215 13 0.23777174035573267 2215 14 0.3359732100512076 2215 15 0.4189079283497037 2215 16 0.34373284009775773 2215 17 0.3633579494584751 2215 18 0.2556069380322654 2215 19 0.3727700771671181 2215 20 0.2727380198021123 2215 21 0.3004013956413658 2215 22 0.3983044058953027 2215 23 0.48138494382209984 2215 24 0.4643736576199231 2215 25 0.4847452693098475 2216 1 -0.33792173189487684 2216 2 -0.20024246143516677 2216 3 -1.9043208941136065 2216 4 -1.2937817258691027 2216 5 -0.5548808670302776 2216 6 -0.2852353400025436 2216 7 -0.28861597127760774 2216 8 -0.38652313962000306 2216 9 -0.2878427790573994 2216 10 -0.5572859653580053 2216 11 -0.5694517471164602 2216 12 -0.3413450442469568 2216 13 -0.50303928782143 2216 14 -0.7168913034682802 2216 15 -0.5041092996496772 2216 16 -0.47037831647809014 2216 17 -0.4744881508430083 2216 18 -0.39913699360131716 2216 19 -0.3640220251379418 2216 20 -0.4104535105879279 2216 21 -0.47534301102031407 2216 22 -0.6322831709440209 2216 23 -0.8413496189403986 2216 24 -0.6749977061810526 2216 25 -0.6764185965723186 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18058.0 3 12.0 4 296.0 5 2008.0 6 2630.0 7 1995.0 8 1564.0 9 1316.0 10 1082.0 11 1009.0 12 921.0 13 892.0 14 880.0 15 876.0 16 870.0 17 948.0 18 1103.0 19 1308.0 20 1681.0 21 2143.0 22 2771.0 23 3317.0 24 3885.0 25 4730.0 26 5599.0 27 6030.0 28 6863.0 29 8346.0 30 10754.0 31 13705.0 32 17459.0 33 23673.0 34 32149.0 35 43756.0 36 65192.0 37 112897.0 38 269965.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.762729143938934 20.22464552174942 10.787271376969592 28.225353957342058 2 13.00186623250221 21.113280610528882 42.32061695801126 23.564236198957648 3 18.7278647575717 25.00215590942207 29.22372392464725 27.04625540835898 4 11.487283014436219 17.886433877472747 39.084382985745144 31.54190012234589 5 11.997032667417914 39.46106342301734 36.01577621911878 12.526127690445962 6 30.669146644869905 39.02848295735909 18.11254168928876 12.189828708482242 7 27.671944140457725 31.901407194176144 21.806008231431697 18.620640433934437 8 23.464423874614564 40.668942365160405 19.836488684446056 16.030145075778975 9 25.58544066201516 16.272061348799383 20.514917196069657 37.627580793115804 10 14.115131999512379 30.89835190157198 34.65645041878886 20.330065680126783 11 33.351817438489554 23.433880918754184 25.12971084516465 18.084590797591616 12 23.303467765553062 25.748757583460808 32.394403885348325 18.5533707656378 13 29.017809359576617 23.21296901944461 25.710441814830233 22.05877980614854 14 19.19016832594253 24.730816744291147 27.99089615762551 28.088118772140813 15 21.423824864308788 34.22480960118454 23.165102494993807 21.186263039512866 16 19.799101203103422 29.9213441220675 30.36137700560596 19.91817766922312 17 19.733098254450095 31.059252365902463 29.07361873812589 20.134030641521548 18 19.493072486174704 29.120235476006425 32.192275673425705 19.194416364393174 19 22.381452157185713 27.945299562051606 28.382207343142824 21.291040937619858 20 22.441346379694295 28.970473242769945 29.42477678770526 19.163403589830498 21 20.871072679587748 28.042837007653926 28.129813439271125 22.956276873487205 22 21.38752040340012 30.632750690519444 28.157881435804732 19.821847470275703 23 20.189598964148043 30.108981496484972 29.106429031207863 20.594990508159118 24 21.833347703669787 28.153570334868483 30.350745225827357 19.66233673563437 25 20.837304035621102 29.272488050725876 29.62230630282397 20.267901610829053 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 286.0 1 286.0 2 323.0 3 360.0 4 360.0 5 360.0 6 555.5 7 751.0 8 751.0 9 751.0 10 1190.0 11 1629.0 12 1629.0 13 1629.0 14 2650.5 15 3672.0 16 3672.0 17 3672.0 18 6366.5 19 9061.0 20 9061.0 21 9061.0 22 14462.5 23 19864.0 24 19864.0 25 19864.0 26 28519.0 27 37174.0 28 37174.0 29 37174.0 30 49389.0 31 61604.0 32 61604.0 33 61604.0 34 69248.0 35 76892.0 36 76892.0 37 76892.0 38 80975.5 39 85059.0 40 85059.0 41 85059.0 42 87731.5 43 90404.0 44 90404.0 45 90404.0 46 91104.5 47 91805.0 48 91805.0 49 91805.0 50 84600.0 51 77395.0 52 77395.0 53 77395.0 54 64252.0 55 51109.0 56 51109.0 57 51109.0 58 41919.0 59 32729.0 60 32729.0 61 32729.0 62 25492.5 63 18256.0 64 18256.0 65 18256.0 66 13623.0 67 8990.0 68 8990.0 69 8990.0 70 6317.0 71 3644.0 72 3644.0 73 3644.0 74 2492.5 75 1341.0 76 1341.0 77 1341.0 78 911.0 79 481.0 80 481.0 81 481.0 82 303.5 83 126.0 84 126.0 85 126.0 86 82.5 87 39.0 88 39.0 89 39.0 90 24.5 91 10.0 92 10.0 93 10.0 94 6.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11595357694486111 2 0.11015589809761804 3 0.0167984028138068 4 0.0 5 0.003716460799514779 6 0.3340354966603883 7 0.020663522045302172 8 0.01025743180666079 9 0.01040609023864138 10 0.004311094527437144 11 0.004905728255359508 12 2.9731686396118227E-4 13 4.4597529594177346E-4 14 0.0 15 0.002378534911689458 16 8.919505918835469E-4 17 1.4865843198059113E-4 18 4.4597529594177346E-4 19 1.4865843198059113E-4 20 7.432921599029558E-4 21 0.012933283582311429 22 1.4865843198059113E-4 23 0.0 24 1.4865843198059113E-4 25 0.007135604735068375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 672683.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.86613507891705 #Duplication Level Percentage of deduplicated Percentage of total 1 93.35026442253462 76.42225356867844 2 5.07828458172185 8.314790630728455 3 0.7116872007183052 1.7478924152382336 4 0.2601543201260506 0.8519131485121236 5 0.14621848476792118 0.5985171112522605 6 0.08536891297166473 0.41932937765271705 7 0.056473043009959076 0.32362608371595614 8 0.04458914117003456 0.29202725232631604 9 0.02673957977788886 0.19701594450451154 >10 0.1959989369073949 3.1069969278495115 >50 0.0229298212189551 1.3134212781555374 >100 0.01765197301248592 3.0726972051005808 >500 0.0021837492377915005 1.2097115782047596 >1k 0.0014558328251943337 2.129807478080695 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2474 0.3677809607199825 No Hit CTGTAGGACGTGGAATATGGCAAGA 2399 0.35663157832143816 No Hit GTATCAACGCAGAGTACTTTTTTTT 1911 0.2840862635149097 No Hit GTCCTACAGTGTGCATTTCTCATTT 1702 0.25301665123096617 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1656 0.24617836335985893 No Hit CTTTAGGACGTGAAATATGGCGAGG 1577 0.23443434723339224 No Hit CTGTAGGACCTGGAATATGGCGAGA 1426 0.211986924004323 No Hit GGTATCAACGCAGAGTACTTTTTTT 1151 0.17110585520966043 No Hit TATCAACGCAGAGTACTTTTTTTTT 983 0.14613123863692112 No Hit GTACATGGGAAGCAGTGGTATCAAC 856 0.12725161777538602 No Hit CTGAAGGACCTGGAATATGGCGAGA 846 0.12576503345558013 No Hit CACACACACACACACACACACACAC 703 0.10450687768235559 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGGCGT 40 2.7023637E-4 16.669613 6 AGGCGTG 40 2.7818594E-4 16.60639 7 ATTAGGC 40 0.005181998 14.2882395 3 TCCAACG 60 2.6003096E-5 14.228757 18 CCAGACT 55 1.9135352E-4 13.855263 4 TAGGACC 395 0.0 13.745647 4 GGACGTG 945 0.0 12.902254 6 AGGACGT 995 0.0 12.5411005 5 GACGTGA 425 0.0 12.503634 7 TGTAGGA 1225 0.0 12.298795 2 AGGACAC 70 1.0622053E-4 12.247062 5 TAGGACG 1020 0.0 12.233721 4 GGACCTG 745 0.0 12.146602 6 GTCCTAA 515 0.0 12.037743 1 GACGTGG 565 0.0 11.924689 7 CTGTAGG 1200 0.0 11.921995 1 TAAGAGT 80 2.78327E-5 11.906866 4 GCCATAT 345 0.0 11.887438 1 GTAGGAC 1170 0.0 11.886512 3 AGGACCT 785 0.0 11.770354 5 >>END_MODULE