FastQCFastQC Report
Thu 26 May 2016
SRR1926969_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926969_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482673
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11310.23432012977730263No Hit
GTCCTACAGTGGACATTTCTAAATT10080.20883703874051374No Hit
CTGTAGGACGTGGAATATGGCAAGA9320.1930913889942052No Hit
GTCCTACAGTGTGCATTTCTCATTT8020.1661580407439405No Hit
GTCCTAAAGTGTGTATTTCTCATTT7780.1611857302977378No Hit
GGTATCAACGCAGAGTACTTTTTTT6940.14378264373602834No Hit
CTTTAGGACGTGAAATATGGCGAGG5870.12161442633004124No Hit
TATCAACGCAGAGTACTTTTTTTTT5410.11208416464148607No Hit
GTACATGGGAAGCAGTGGTATCAAC4830.1000677477298295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG653.430494E-614.58637418
CCAACGA653.430494E-614.58637419
TAGTACT400.005186111714.28535654
CTAGGAC551.893158E-413.8712343
GGCCTCG500.00151951513.27360215
GGCGAGG2100.013.09300819
AGGACGT3650.013.0364915
TAGGACC2250.012.6980954
TAAGGGT603.9984199E-412.6980944
GTAGGAC5250.012.3520043
CTTACAC550.002976464812.137333
AGGACGA550.003023264712.1120855
ATCCCGT550.00310427312.06941110
CTCGAAC550.003109095612.0669118
TCGAACT550.003109095612.0669119
TAGGACG4500.012.063194
GGACGTG3800.011.7705796
GTATAGG657.6848554E-411.7494421
TGTAGGA5850.011.7384212
CTGTAGG5550.011.6965171
Produced by FastQC (version 0.11.5)