FastQCFastQC Report
Thu 26 May 2016
SRR1926969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926969_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482673
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19010.39384842325963954No Hit
GGTATCAACGCAGAGTACTTTTTTT11580.23991397902928072No Hit
GTCCTACAGTGGACATTTCTAAATT10590.21940319843869452No Hit
CTGTAGGACGTGGAATATGGCAAGA10320.21380934918671649No Hit
TATCAACGCAGAGTACTTTTTTTTT9710.2011713934692846No Hit
GTCCTAAAGTGTGTATTTCTCATTT8280.17154471039399344No Hit
GTCCTACAGTGTGCATTTCTCATTT8070.16719393875356608No Hit
CTTTAGGACGTGAAATATGGCGAGG7550.1564205994534602No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6630.1373600760763498No Hit
GTACATGGGAAGCAGTGGTATCAAC6560.13590981886287404No Hit
CTGTAGGACCTGGAATATGGCGAGA6310.13073032881474622No Hit
CCCATGTACTCTGCGTTGATACCAC5070.10504005817603221No Hit
CACACACACACACACACACACACAC4940.10234672335100575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG500.001452408313.35337451
TAGGACC2800.012.9270934
GGCGAGG2000.012.33148319
GTCTAGA550.002972609112.1394331
AGGGTGT550.003002906212.1230156
TGGCGAG5450.012.00948818
AGGACCT4050.011.9947295
GGACCTG4100.011.616136
TTGAACA752.0178196E-411.4302714
ATGGCGA5800.011.28477917
GGCGAGA3550.011.22258419
CTTATAG600.005746548511.1139213
CAGGAGT600.00575100211.1127644
TAGGGCT600.00575100211.1127644
AGATCAC600.005927018411.067862510
GCTGTAC600.005927018411.06786258
TATGGCG6000.011.06671516
GTAGGAC6050.011.022073
GTACAAG700.001439245310.9007151
TTAGACT700.001455595410.8859734