##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926969_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 482673 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.696523319100095 31.0 30.0 33.0 25.0 34.0 2 29.94875412546382 31.0 30.0 33.0 26.0 34.0 3 30.05513256386829 31.0 30.0 34.0 26.0 34.0 4 33.62431294064512 35.0 35.0 37.0 28.0 37.0 5 33.59550047340539 35.0 35.0 37.0 28.0 37.0 6 33.55929169437694 37.0 35.0 37.0 28.0 37.0 7 33.55630209272116 37.0 35.0 37.0 28.0 37.0 8 33.57710292475444 37.0 35.0 37.0 28.0 37.0 9 35.06776016060563 39.0 35.0 39.0 28.0 39.0 10 34.947720299250214 39.0 35.0 39.0 28.0 39.0 11 35.0003770668755 39.0 35.0 39.0 28.0 39.0 12 34.88554777250851 39.0 35.0 39.0 27.0 39.0 13 34.9027664692245 39.0 35.0 39.0 27.0 39.0 14 35.946178883011896 40.0 36.0 41.0 27.0 41.0 15 35.95565942159599 40.0 36.0 41.0 27.0 41.0 16 35.92590221537149 40.0 36.0 41.0 27.0 41.0 17 35.90573120932805 40.0 36.0 41.0 27.0 41.0 18 35.87886830214244 40.0 36.0 41.0 27.0 41.0 19 35.96841754148254 40.0 36.0 41.0 27.0 41.0 20 35.938125811885065 40.0 36.0 41.0 27.0 41.0 21 35.87740147056081 40.0 36.0 41.0 27.0 41.0 22 35.789847370787264 40.0 36.0 41.0 27.0 41.0 23 35.72105752756006 40.0 36.0 41.0 27.0 41.0 24 35.65469790106345 40.0 36.0 41.0 27.0 41.0 25 35.533255433803006 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.23402109926038506 1101 2 -0.10849403476922959 1101 3 -0.15234932267105705 1101 4 -0.3455825879992602 1101 5 -0.32101506406841196 1101 6 -0.08204310292388328 1101 7 -0.09922696557447352 1101 8 -0.11960019673052358 1101 9 -0.041370497139745055 1101 10 -0.42377808105176484 1101 11 -0.465792085373792 1101 12 -0.18527268036167044 1101 13 -0.22800481459828603 1101 14 -0.19494475993278115 1101 15 -0.24859820102800967 1101 16 -0.15153199901786962 1101 17 -0.3262268955984382 1101 18 -0.3566289904423954 1101 19 -0.1798012935506037 1101 20 -0.5767356516682511 1101 21 -0.5406603795015741 1101 22 -0.2600469342208669 1101 23 -0.29418077600347914 1101 24 -0.358201105589778 1101 25 -0.2786357715759493 1102 1 -0.6773974393684412 1102 2 -0.6604489932653799 1102 3 -0.5201863439509324 1102 4 -0.44109924734398476 1102 5 -0.5398081768186103 1102 6 -0.5351518453225452 1102 7 -0.5796215026350566 1102 8 -0.7379073637555749 1102 9 -0.434040462533936 1102 10 -0.43311548384335197 1102 11 -0.5003400903372963 1102 12 -0.3063140146507095 1102 13 -0.36072849992434186 1102 14 -0.6085073085851604 1102 15 -0.5834055568820986 1102 16 -0.47134093486995 1102 17 -0.13474269943205286 1102 18 -0.17137037174600778 1102 19 -0.34283011117258866 1102 20 -0.46718398573377584 1102 21 -0.3955895878840394 1102 22 -0.37353510735781725 1102 23 -0.35774293929048895 1102 24 -0.3075409747197 1102 25 -0.27519253204054905 1103 1 -1.2362929345410052 1103 2 -1.3067097760970086 1103 3 -1.005902744764466 1103 4 -1.166251005715047 1103 5 -1.423164627815659 1103 6 -1.183234118230466 1103 7 -1.2420204927308305 1103 8 -1.1310725875163783 1103 9 -1.2385974995247935 1103 10 -1.2479410493819287 1103 11 -1.345143291484348 1103 12 -1.279708570830529 1103 13 -1.3764439656413643 1103 14 -1.3521467042011182 1103 15 -1.2116072883691373 1103 16 -1.1620986747416922 1103 17 -1.3430958386289618 1103 18 -1.2871606451838105 1103 19 -1.3242703935717373 1103 20 -1.4026798596204273 1103 21 -1.259262257350798 1103 22 -1.2678360837544247 1103 23 -1.2046734849972296 1103 24 -1.1264965498430186 1103 25 -1.0407346152797174 1104 1 -0.222713484625082 1104 2 -0.2351868359096443 1104 3 -0.006308101478374795 1104 4 0.044474432670732256 1104 5 0.039009585492053134 1104 6 -0.07778882637362727 1104 7 -0.1139127579241972 1104 8 -0.2308053523494351 1104 9 0.006042783924641526 1104 10 -0.2704533602154626 1104 11 -0.0638557584552828 1104 12 -0.23800937553867385 1104 13 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0.10440658547756954 2214 16 0.4827918562208424 2214 17 0.5530799424603785 2214 18 0.7107041259388467 2214 19 0.7231262037713933 2214 20 0.6876543739592194 2214 21 0.6078840075409673 2214 22 0.6799827610970368 2214 23 0.5902108767646936 2214 24 0.5270651353084048 2214 25 0.554212809421756 2215 1 0.2042532609625205 2215 2 0.2780503573749016 2215 3 0.21923537678249616 2215 4 -0.10726900254235261 2215 5 0.2490155045622231 2215 6 0.23044675261905212 2215 7 0.10824070742233971 2215 8 0.2008293872114777 2215 9 0.06757420880626341 2215 10 0.04415867163989873 2215 11 -0.08871585316040864 2215 12 0.11117540707002149 2215 13 0.20078785975111657 2215 14 -0.06346583335160005 2215 15 0.15743707463069967 2215 16 0.08940561933412994 2215 17 0.054372844703458156 2215 18 -0.039553840304428434 2215 19 -0.15732793965783998 2215 20 0.009086455132596427 2215 21 0.1487501599130283 2215 22 0.08100971242740229 2215 23 -0.0294916021680649 2215 24 0.07476432210052764 2215 25 0.16284423708065532 2216 1 0.007437902482973158 2216 2 0.1899501264402801 2216 3 -1.4262069824668338 2216 4 -1.1260291225221835 2216 5 -0.0067744511089458115 2216 6 0.5057035678448116 2216 7 0.2904102983760737 2216 8 0.3261578718025646 2216 9 0.2584104841227983 2216 10 -0.10412059998699874 2216 11 0.15269045905257883 2216 12 0.050853691252328304 2216 13 -0.06805521088302413 2216 14 -0.12366478220091182 2216 15 0.018000342753758503 2216 16 0.013197194202071216 2216 17 0.32206124222953747 2216 18 0.3209629754522112 2216 19 0.09605976506437486 2216 20 0.019405516529353406 2216 21 0.0360401288562997 2216 22 0.3128335650539569 2216 23 -0.04707050483044384 2216 24 0.01871622399313111 2216 25 -0.2972963145080172 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12841.0 3 7.0 4 203.0 5 1364.0 6 1909.0 7 1428.0 8 1071.0 9 963.0 10 824.0 11 751.0 12 746.0 13 649.0 14 688.0 15 616.0 16 689.0 17 703.0 18 792.0 19 944.0 20 1196.0 21 1579.0 22 1979.0 23 2400.0 24 2863.0 25 3315.0 26 3989.0 27 4470.0 28 5137.0 29 6184.0 30 7854.0 31 10323.0 32 13100.0 33 17575.0 34 23664.0 35 32875.0 36 48524.0 37 83353.0 38 185105.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.346361253069624 20.307211123647704 10.614048914847016 27.732378708435657 2 13.27421699539415 21.705345977814783 41.642801002876325 23.377636023914743 3 18.200655618133517 26.110344427453075 29.55254684022727 26.136453114186136 4 11.219604162652562 18.411429684279003 39.415090547845026 30.953875605223413 5 11.840221447322348 39.65995570224783 35.943129808160485 12.556693042269337 6 29.614892887211948 39.59982121883023 18.65207313397154 12.13321275998628 7 26.93823589139994 32.876757746934175 22.406947685141056 17.77805867652483 8 23.95985280466782 39.40790346107943 20.406983490253282 16.22526024399947 9 26.082109808504377 16.07862882338559 20.63436809760019 37.20489327050984 10 13.652401819104742 31.308491572655416 34.9784007210119 20.060705887227936 11 32.79691200328193 23.912912856914943 25.07029004875239 18.219885091050735 12 22.385319960469804 26.457883145592525 32.67560170634534 18.481195187592327 13 28.25165900441089 23.608725648425732 26.108989804607297 22.030625542556077 14 19.47177488693173 24.397884281905142 28.643201504952625 27.487139326210496 15 21.780180210044772 33.05211339682882 24.004317748141027 21.163388644985385 16 19.995856382207304 30.215260944330495 29.64468477427642 20.144197899185777 17 19.80723969900885 30.44365531872576 29.0601070706401 20.688997911625286 18 19.365197754159155 29.380943501771394 31.61414631114426 19.639712432925187 19 21.760280769796527 28.45446088759885 29.09153816351857 20.693720179086046 20 21.946509927092894 28.929556279769365 29.933349769717964 19.190584023419778 21 21.34848456592506 28.048429240718527 28.63069077768177 21.972395415674647 22 21.249168691847274 30.045807409985642 28.715300006422567 19.989723891744514 23 20.293656367768655 29.592498440973497 29.42447578381223 20.68936940744562 24 21.24051115457288 28.41204793317201 30.451735339940996 19.895705572314117 25 20.908173603873703 29.017754470732783 29.6358147601002 20.438257165293315 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 236.0 1 236.0 2 252.0 3 268.0 4 268.0 5 268.0 6 447.5 7 627.0 8 627.0 9 627.0 10 994.5 11 1362.0 12 1362.0 13 1362.0 14 2074.5 15 2787.0 16 2787.0 17 2787.0 18 4846.0 19 6905.0 20 6905.0 21 6905.0 22 10921.5 23 14938.0 24 14938.0 25 14938.0 26 21235.0 27 27532.0 28 27532.0 29 27532.0 30 35764.0 31 43996.0 32 43996.0 33 43996.0 34 49719.5 35 55443.0 36 55443.0 37 55443.0 38 58836.0 39 62229.0 40 62229.0 41 62229.0 42 63719.5 43 65210.0 44 65210.0 45 65210.0 46 65640.5 47 66071.0 48 66071.0 49 66071.0 50 60870.0 51 55669.0 52 55669.0 53 55669.0 54 46127.0 55 36585.0 56 36585.0 57 36585.0 58 29501.0 59 22417.0 60 22417.0 61 22417.0 62 17078.0 63 11739.0 64 11739.0 65 11739.0 66 8611.5 67 5484.0 68 5484.0 69 5484.0 70 3806.0 71 2128.0 72 2128.0 73 2128.0 74 1429.0 75 730.0 76 730.0 77 730.0 78 477.5 79 225.0 80 225.0 81 225.0 82 144.0 83 63.0 84 63.0 85 63.0 86 44.0 87 25.0 88 25.0 89 25.0 90 13.5 91 2.0 92 2.0 93 2.0 94 1.5 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1095980094183847 2 0.09530261688555192 3 0.015538470144383466 4 0.0 5 0.006215388057753386 6 0.33936018795333484 7 0.01968206218288572 8 0.01015180049433053 9 0.010566159698180757 10 0.003729232834652032 11 0.006215388057753386 12 8.287184077004514E-4 13 8.287184077004514E-4 14 0.0 15 0.00290051442695158 16 6.215388057753386E-4 17 2.0717960192511285E-4 18 6.215388057753386E-4 19 0.0 20 8.287184077004514E-4 21 0.014916931338608127 22 0.0 23 0.0 24 2.0717960192511285E-4 25 0.0070441064654538366 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 482673.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.16441052791224 #Duplication Level Percentage of deduplicated Percentage of total 1 94.276550788872 80.29006874539063 2 4.421455533102046 7.531013083040233 3 0.5940687484422924 1.5178054432242722 4 0.22880486676667844 0.7794412641640664 5 0.10154112779506771 0.4323845146503173 6 0.06985148432042161 0.3569316291989046 7 0.04844988597800106 0.28883441856227143 8 0.03417910660269455 0.23286747729513232 9 0.02960458253242782 0.22691311382692278 >10 0.16068198329349972 2.7391492019128556 >50 0.01777110855532561 1.0204491904520234 >100 0.014119506527042856 2.3102118129176237 >500 0.0019475181416609571 1.2062112830973928 >1k 9.737590708304786E-4 1.0677188222673726 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1901 0.39384842325963954 No Hit GGTATCAACGCAGAGTACTTTTTTT 1158 0.23991397902928072 No Hit GTCCTACAGTGGACATTTCTAAATT 1059 0.21940319843869452 No Hit CTGTAGGACGTGGAATATGGCAAGA 1032 0.21380934918671649 No Hit TATCAACGCAGAGTACTTTTTTTTT 971 0.2011713934692846 No Hit GTCCTAAAGTGTGTATTTCTCATTT 828 0.17154471039399344 No Hit GTCCTACAGTGTGCATTTCTCATTT 807 0.16719393875356608 No Hit CTTTAGGACGTGAAATATGGCGAGG 755 0.1564205994534602 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 663 0.1373600760763498 No Hit GTACATGGGAAGCAGTGGTATCAAC 656 0.13590981886287404 No Hit CTGTAGGACCTGGAATATGGCGAGA 631 0.13073032881474622 No Hit CCCATGTACTCTGCGTTGATACCAC 507 0.10504005817603221 No Hit CACACACACACACACACACACACAC 494 0.10234672335100575 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAGG 50 0.0014524083 13.3533745 1 TAGGACC 280 0.0 12.927093 4 GGCGAGG 200 0.0 12.331483 19 GTCTAGA 55 0.0029726091 12.139433 1 AGGGTGT 55 0.0030029062 12.123015 6 TGGCGAG 545 0.0 12.009488 18 AGGACCT 405 0.0 11.994729 5 GGACCTG 410 0.0 11.61613 6 TTGAACA 75 2.0178196E-4 11.430271 4 ATGGCGA 580 0.0 11.284779 17 GGCGAGA 355 0.0 11.222584 19 CTTATAG 60 0.0057465485 11.113921 3 CAGGAGT 60 0.005751002 11.112764 4 TAGGGCT 60 0.005751002 11.112764 4 AGATCAC 60 0.0059270184 11.0678625 10 GCTGTAC 60 0.0059270184 11.0678625 8 TATGGCG 600 0.0 11.066715 16 GTAGGAC 605 0.0 11.02207 3 GTACAAG 70 0.0014392453 10.900715 1 TTAGACT 70 0.0014555954 10.885973 4 >>END_MODULE