FastQCFastQC Report
Thu 26 May 2016
SRR1926968_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926968_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786537
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10820.13756504779813283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAC704.525682E-714.89979418
TCGAACT707.397599E-613.54526619
TAGGACC1204.9112714E-1113.4933084
GTCTTAG1153.4742698E-1013.2839861
CCTCGAA905.5212513E-712.64224817
GCCTCGA854.0221476E-612.27041716
GTCCTAC3650.012.0329261
TCCTACA4300.011.9750062
CCTAAAC907.1430204E-611.6546873
CCTACAG3850.011.3932493
TCTTAGG1451.1386874E-911.179732
GGCCTCG855.4203607E-511.15492515
CTTAGGC803.6380164E-410.7276093
GGTCTAC909.0282454E-510.6087391
GACGTGA1002.3513267E-510.4671297
GTCCTGA1303.5739868E-710.2823161
CCTACAC1303.6373785E-710.2691653
CTACACT1303.6953134E-710.2573574
TAAACCC750.00258871710.1596684
GTATAGA1605.657057E-910.1446061
Produced by FastQC (version 0.11.5)