##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926968_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 786537 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.197749120511812 31.0 28.0 31.0 16.0 34.0 2 28.228368150512946 31.0 28.0 31.0 16.0 34.0 3 27.78334649228199 31.0 28.0 31.0 16.0 34.0 4 31.645707703515537 35.0 32.0 37.0 19.0 37.0 5 31.447853057135266 35.0 32.0 37.0 18.0 37.0 6 31.07305441447764 35.0 32.0 37.0 16.0 37.0 7 30.890518818567976 35.0 31.0 37.0 16.0 37.0 8 31.064199141299138 35.0 32.0 37.0 13.0 37.0 9 33.224374695659584 37.0 33.0 39.0 17.0 39.0 10 33.41814180388208 37.0 34.0 39.0 17.0 39.0 11 33.56986638899378 38.0 34.0 39.0 20.0 39.0 12 33.47379207844005 38.0 34.0 39.0 18.0 39.0 13 33.54748854790048 38.0 34.0 39.0 19.0 39.0 14 34.57763080439954 39.0 34.0 41.0 19.0 41.0 15 34.1647436802083 39.0 33.0 41.0 17.0 41.0 16 34.23510782073825 39.0 34.0 41.0 17.0 41.0 17 34.45748388187714 39.0 34.0 41.0 18.0 41.0 18 34.49373773897477 39.0 34.0 41.0 18.0 41.0 19 33.89971355447995 39.0 33.0 41.0 11.0 41.0 20 34.19788515988441 39.0 34.0 41.0 17.0 41.0 21 34.173718464611326 39.0 34.0 41.0 14.0 41.0 22 34.19227194652 39.0 34.0 41.0 11.0 41.0 23 34.17978556634971 39.0 34.0 41.0 9.0 41.0 24 32.88890795982897 39.0 32.0 41.0 2.0 41.0 25 33.471038234692074 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.061255334907158954 1101 2 -0.32046094834594285 1101 3 -1.4943363680853388 1101 4 -0.23649740632697203 1101 5 -0.3546808424057204 1101 6 -2.1222806420699527 1101 7 -0.479509911078825 1101 8 -0.01240304970242434 1101 9 -0.07682233722024279 1101 10 -8.732823325715437 1101 11 -3.8659598147947456 1101 12 -1.3514941424177493 1101 13 -0.49003447335605443 1101 14 -0.42264543662904686 1101 15 -1.9143849883043274 1101 16 -0.2225111637410606 1101 17 -0.08214983636232631 1101 18 0.1034726180337131 1101 19 -0.8429925149275803 1101 20 -0.049677080494831216 1101 21 -0.04539986674979701 1101 22 -0.06263009593986624 1101 23 -0.025323696424827347 1101 24 -0.2627446069926691 1101 25 -0.010946529310906783 1102 1 0.01401345838442225 1102 2 -0.27812859945136026 1102 3 -0.44677344703020694 1102 4 -0.2287560705740539 1102 5 -0.13807911311451804 1102 6 -1.0576271823014736 1102 7 -0.271332249535984 1102 8 0.5816901695308978 1102 9 0.5268017020624214 1102 10 0.5195154576131529 1102 11 0.23470868435128267 1102 12 0.31100274414643536 1102 13 0.1393020979505195 1102 14 0.09351626040103156 1102 15 0.6662203822241821 1102 16 0.5033574848498859 1102 17 0.37847706135092807 1102 18 0.06646202049125094 1102 19 0.5179177054443755 1102 20 0.4336111821486881 1102 21 0.27260572557395335 1102 22 0.23196616556501937 1102 23 -0.2359075990721351 1102 24 -3.707642723512258 1102 25 -1.7533638305254442 1103 1 -0.450814384838381 1103 2 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0.007006486403433598 2215 3 0.3575851947135611 2215 4 0.5155569608087873 2215 5 0.8981291577031847 2215 6 1.3289023928006323 2215 7 1.0331071415617892 2215 8 -0.36791507032073056 2215 9 0.1844114169911535 2215 10 -0.013214730142117048 2215 11 -0.15861816298555453 2215 12 -0.2721645320476114 2215 13 -0.05943899357236404 2215 14 -0.09545155446934928 2215 15 -0.05912762144340178 2215 16 -0.02764049902108212 2215 17 -0.32232965074376096 2215 18 -0.9095938424328125 2215 19 0.3399936671431334 2215 20 -3.5132609525516543 2215 21 -1.8882346147194582 2215 22 -1.121726424249097 2215 23 -1.0321144632881882 2215 24 -0.7198983915959474 2215 25 -0.5773426116790645 2216 1 -0.28452569293082064 2216 2 -0.7880460855521001 2216 3 0.02230458591311546 2216 4 -0.2559238108030826 2216 5 4.4543878988889674E-4 2216 6 -1.448949090234425 2216 7 -9.245119435246714 2216 8 -10.762468899218582 2216 9 -5.693581979789705 2216 10 -3.24222306512355 2216 11 -2.2569420344687323 2216 12 -1.8050139505262024 2216 13 -1.575655426321326 2216 14 -1.544372970783101 2216 15 -2.6326759749245987 2216 16 -1.6606116619014202 2216 17 -2.0459833050087113 2216 18 -1.6129739443664377 2216 19 -1.0129984270342618 2216 20 -1.7973551277011168 2216 21 -4.407277556933725 2216 22 -3.035625735522025 2216 23 -2.802865216697228 2216 24 -3.262241408268711 2216 25 -2.7287062554279444 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44237.0 3 9.0 4 349.0 5 1952.0 6 2482.0 7 1934.0 8 1635.0 9 1424.0 10 1456.0 11 1448.0 12 1387.0 13 1395.0 14 1363.0 15 1474.0 16 1528.0 17 1717.0 18 1890.0 19 2379.0 20 3188.0 21 4126.0 22 5131.0 23 6138.0 24 7329.0 25 8109.0 26 9612.0 27 10356.0 28 11466.0 29 13874.0 30 16387.0 31 20152.0 32 25704.0 33 34082.0 34 46297.0 35 65075.0 36 94254.0 37 148808.0 38 186390.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.23802423825362 19.437499203527416 13.416548788724498 24.90792776949446 2 13.93874817960547 20.92511686169075 42.135131832208 23.001003126495778 3 18.755195490473394 25.41331598638893 29.445334847371925 26.38615367576575 4 11.321354827300613 18.0381135824961 39.5008263159033 31.139705274299985 5 12.47137158255221 38.264097389393534 36.02494503216683 13.239585995887426 6 29.899105762187677 38.77332241393343 19.181350207536422 12.146221616342476 7 26.98940581787392 31.9749707984147 22.93892915618895 18.09669422752243 8 23.975645521198597 36.80879821485496 22.268281797896076 16.947274466050366 9 25.738715334073415 16.416094120160377 21.00447737090205 36.840713174864156 10 13.70367920880731 30.17363301520116 35.29041431798295 20.832273458008586 11 32.99602246139448 24.060548106854387 24.553035543529127 18.390393888222007 12 21.973481924753976 26.127310667377067 32.31469201062554 19.58451539724341 13 27.53053374447659 22.90072161998919 26.398067202848335 23.17067743268589 14 20.285881773987835 22.785853162921583 28.81261531849203 28.11564974459855 15 22.762530136414963 30.710659898477154 24.11803302035543 22.408776944752447 16 21.33788578186396 29.888732054069862 27.7263190020219 21.047063162044278 17 21.06619769532631 29.410530492038422 28.08093061818256 21.44234119445271 18 20.757632015299635 29.19430231020416 29.280515345411633 20.767550329084568 19 22.524349875182647 28.311474930342634 28.243947661916017 20.920227532558698 20 22.320108137939453 28.18440417953645 29.0451587799131 20.450328902611005 21 22.48432451403132 27.80057450290494 28.01852447613804 21.696576506925698 22 21.787489986139548 28.513116567693697 28.457166109281417 21.24222733688534 23 21.646517861797893 28.266821295550987 28.15084963231133 21.93581121033979 24 21.811241195761504 28.312930515977015 28.65855131731372 21.21727697094776 25 21.970405014133725 28.09044056779037 28.207935484609308 21.731218933466597 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 214.0 1 214.0 2 287.5 3 361.0 4 361.0 5 361.0 6 554.0 7 747.0 8 747.0 9 747.0 10 1174.0 11 1601.0 12 1601.0 13 1601.0 14 2886.0 15 4171.0 16 4171.0 17 4171.0 18 7628.5 19 11086.0 20 11086.0 21 11086.0 22 17253.5 23 23421.0 24 23421.0 25 23421.0 26 32425.0 27 41429.0 28 41429.0 29 41429.0 30 52522.0 31 63615.0 32 63615.0 33 63615.0 34 73319.0 35 83023.0 36 83023.0 37 83023.0 38 90190.5 39 97358.0 40 97358.0 41 97358.0 42 100790.0 43 104222.0 44 104222.0 45 104222.0 46 105104.0 47 105986.0 48 105986.0 49 105986.0 50 99384.5 51 92783.0 52 92783.0 53 92783.0 54 79685.0 55 66587.0 56 66587.0 57 66587.0 58 55132.0 59 43677.0 60 43677.0 61 43677.0 62 34526.0 63 25375.0 64 25375.0 65 25375.0 66 18963.5 67 12552.0 68 12552.0 69 12552.0 70 8910.5 71 5269.0 72 5269.0 73 5269.0 74 3637.5 75 2006.0 76 2006.0 77 2006.0 78 1378.5 79 751.0 80 751.0 81 751.0 82 474.5 83 198.0 84 198.0 85 198.0 86 139.0 87 80.0 88 80.0 89 80.0 90 47.0 91 14.0 92 14.0 93 14.0 94 9.0 95 4.0 96 4.0 97 4.0 98 5.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23228405020997103 2 0.12624962334893336 3 0.127139600552803 4 0.06585831308635194 5 0.021359452892870903 6 0.021486592493423703 7 0.2966166880896894 8 0.29076826646426046 9 0.017545264876286812 10 0.03572622775533764 11 0.015383891666889162 12 0.01652814807186439 13 0.014239635261913932 14 0.01627386887075878 15 0.014366774862466737 16 0.01868952128126204 17 0.017163846074628404 18 0.014366774862466737 19 0.02390224490392696 20 0.017036706474075597 21 0.015002472865230753 22 0.016146729270205978 23 0.017545264876286812 24 0.016019589669653177 25 0.015256752066336357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 786537.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.05602574081153 #Duplication Level Percentage of deduplicated Percentage of total 1 94.47582129617945 85.08116994533046 2 4.427546846635347 7.974545455784835 3 0.5924626028680509 1.6006448224306018 4 0.17936456666023637 0.64611440128575 5 0.09213312932539887 0.41485717330548183 6 0.05781676427135914 0.312404880688317 7 0.03830179587102169 0.24145152604160272 8 0.022654001134573953 0.16321034474460527 9 0.01564792661332794 0.12682710736921718 >10 0.08300067517612296 1.381255832641979 >50 0.009461566790026596 0.5862549423595889 >100 0.0048004919589224255 0.8599218433903913 >500 8.471455853148319E-4 0.47376440639780276 >1k 1.4119093088580528E-4 0.13757731822931363 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1082 0.13756504779813283 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.2713960055280298E-4 0.0 3 0.0 0.0 0.0 1.2713960055280298E-4 0.0 4 0.0 0.0 0.0 1.2713960055280298E-4 0.0 5 0.0 0.0 0.0 1.2713960055280298E-4 0.0 6 0.0 0.0 0.0 1.2713960055280298E-4 0.0 7 0.0 0.0 0.0 1.2713960055280298E-4 0.0 8 0.0 0.0 0.0 2.5427920110560596E-4 0.0 9 0.0 0.0 0.0 3.8141880165840894E-4 1.2713960055280298E-4 10 0.0 0.0 0.0 3.8141880165840894E-4 1.2713960055280298E-4 11 0.0 0.0 0.0 3.8141880165840894E-4 1.2713960055280298E-4 12 0.0 0.0 0.0 5.085584022112119E-4 1.2713960055280298E-4 13 0.0 0.0 0.0 5.085584022112119E-4 1.2713960055280298E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGAAC 70 4.525682E-7 14.899794 18 TCGAACT 70 7.397599E-6 13.545266 19 TAGGACC 120 4.9112714E-11 13.493308 4 GTCTTAG 115 3.4742698E-10 13.283986 1 CCTCGAA 90 5.5212513E-7 12.642248 17 GCCTCGA 85 4.0221476E-6 12.270417 16 GTCCTAC 365 0.0 12.032926 1 TCCTACA 430 0.0 11.975006 2 CCTAAAC 90 7.1430204E-6 11.654687 3 CCTACAG 385 0.0 11.393249 3 TCTTAGG 145 1.1386874E-9 11.17973 2 GGCCTCG 85 5.4203607E-5 11.154925 15 CTTAGGC 80 3.6380164E-4 10.727609 3 GGTCTAC 90 9.0282454E-5 10.608739 1 GACGTGA 100 2.3513267E-5 10.467129 7 GTCCTGA 130 3.5739868E-7 10.282316 1 CCTACAC 130 3.6373785E-7 10.269165 3 CTACACT 130 3.6953134E-7 10.257357 4 TAAACCC 75 0.002588717 10.159668 4 GTATAGA 160 5.657057E-9 10.144606 1 >>END_MODULE