FastQCFastQC Report
Thu 26 May 2016
SRR1926968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926968_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786537
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18920.24054812424590324No Hit
GGTATCAACGCAGAGTACTTTTTTT11250.14303205062190336No Hit
TATCAACGCAGAGTACTTTTTTTTT9560.12154545812847965No Hit
GTACATGGGAAGCAGTGGTATCAAC9400.11951122451963479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGA953.110472E-1014.9790197
TAGGACC1700.011.7652584
GTAGAAC1302.4974725E-911.7236593
CGTGAAA1157.197377E-811.5483069
AGGACGT1501.6734703E-1011.4291085
CTGGACC1001.8672035E-611.4291084
GGACGTG1451.1623342E-911.166376
TTAGGAC2050.011.15177253
GTCCTAA1552.910383E-1011.075981
ACGTGAA1201.2985765E-711.0664238
TAGGACG1657.6397555E-1110.9673254
TAAGTAG700.001458609510.8848645
GGCGAGG1158.938914E-710.72547219
GGTATCA6100.010.163071
CTTGAAC856.4250286E-410.08579353
TAGGACA1803.7471182E-1010.0533824
GTATTAA1159.770221E-69.9523311
GTAGGAC2700.09.8782673
GACAGGT1252.6092384E-69.866187
ATAGGAC900.00108530459.5254733
Produced by FastQC (version 0.11.5)