##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926968_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 786537 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.038803006088717 31.0 30.0 33.0 26.0 34.0 2 30.34508738940444 31.0 30.0 34.0 26.0 34.0 3 30.408451223527948 31.0 30.0 34.0 26.0 34.0 4 34.00845096924874 37.0 35.0 37.0 30.0 37.0 5 33.9900869253449 37.0 35.0 37.0 30.0 37.0 6 33.94051265229735 37.0 35.0 37.0 30.0 37.0 7 33.946544154947574 37.0 35.0 37.0 30.0 37.0 8 33.965120521984346 37.0 35.0 37.0 30.0 37.0 9 35.52622826389604 39.0 35.0 39.0 30.0 39.0 10 35.426572430794735 39.0 35.0 39.0 30.0 39.0 11 35.45672740125385 39.0 35.0 39.0 30.0 39.0 12 35.36202492699008 39.0 35.0 39.0 30.0 39.0 13 35.38034065784572 39.0 35.0 39.0 30.0 39.0 14 36.502964259786886 40.0 37.0 41.0 30.0 41.0 15 36.47802582713846 40.0 37.0 41.0 30.0 41.0 16 36.44592307799887 40.0 37.0 41.0 30.0 41.0 17 36.42424959029264 40.0 37.0 41.0 30.0 41.0 18 36.39387212553255 40.0 37.0 41.0 30.0 41.0 19 36.48566818852768 40.0 37.0 41.0 30.0 41.0 20 36.44500513008288 40.0 37.0 41.0 30.0 41.0 21 36.400764363278526 40.0 37.0 41.0 30.0 41.0 22 36.31300625399695 40.0 37.0 41.0 30.0 41.0 23 36.252542474162055 40.0 37.0 41.0 30.0 41.0 24 36.18750675429128 40.0 37.0 41.0 29.0 41.0 25 36.077307234116134 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.07699722745255144 1101 2 -0.03997541805882676 1101 3 0.20734273647969914 1101 4 0.12891121309969122 1101 5 0.01672250509945883 1101 6 0.0032617352515558196 1101 7 0.014180948591835829 1101 8 0.10905854386346192 1101 9 0.03124469799505647 1101 10 0.0876679939044891 1101 11 -0.0365736778406216 1101 12 0.1649670223460049 1101 13 0.04746686417152546 1101 14 0.18839885095047038 1101 15 0.02215441825870812 1101 16 0.2956117762053694 1101 17 -0.02712930965207505 1101 18 -0.10350749176657814 1101 19 0.0339145110129806 1101 20 0.20416605408289001 1101 21 0.05425700940958933 1101 22 -0.07688489119770026 1101 23 0.0080228884206619 1101 24 -0.04158673610377406 1101 25 -0.05195245441668561 1102 1 -0.5860862361345909 1102 2 -0.49066230130202015 1102 3 -0.2944437826397639 1102 4 -0.4536089204518774 1102 5 -0.38644445215454937 1102 6 -0.468458630769895 1102 7 -0.46902132391979023 1102 8 -0.3633504845073503 1102 9 -0.4050203058003703 1102 10 -0.33052796278020935 1102 11 -0.10828500007233544 1102 12 -0.2817902601286164 1102 13 8.446455075130643E-4 1102 14 -0.31116749191887294 1102 15 -0.2246876682770207 1102 16 -0.1470098257018364 1102 17 -0.2833754549220302 1102 18 -0.0878936111801849 1102 19 -0.24808123099290214 1102 20 -0.45834639966034985 1102 21 -0.3891036628553337 1102 22 -0.43795597241982875 1102 23 -0.35949967657602144 1102 24 -0.2829187825503041 1102 25 -0.35069922237379814 1103 1 -0.6586893120508215 1103 2 -0.4600076692485011 1103 3 -0.333047258497821 1103 4 -0.46981110922442326 1103 5 -0.5445987206245917 1103 6 -0.7153727159938583 1103 7 -0.5816776648156576 1103 8 -0.6384559237161938 1103 9 -0.6860067077782688 1103 10 -0.1981335837807876 1103 11 -0.5077450786063622 1103 12 -0.6434103881099986 1103 13 -0.41596290975717665 1103 14 -0.3824889360724484 1103 15 -0.39086349724735214 1103 16 -0.4822656066791495 1103 17 -0.35237450960498506 1103 18 -0.3619037551592399 1103 19 -0.5696253700272251 1103 20 -0.45829186213853035 1103 21 -0.5660142940362505 1103 22 -0.46986951226857343 1103 23 -0.5413777670355913 1103 24 -0.5809163465410023 1103 25 -0.533531719465131 1104 1 -0.18200306183433668 1104 2 -0.23967497093730472 1104 3 -0.047982268644851445 1104 4 0.07442728607907867 1104 5 -0.06235491653114167 1104 6 0.08483732978990588 1104 7 0.1060157837986182 1104 8 -0.08411490350650297 1104 9 0.0899303939451741 1104 10 0.17307016062945735 1104 11 0.2276061152796629 1104 12 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9 0.1680259213969748 1107 10 0.39043752229441964 1107 11 0.4605634659309814 1107 12 0.31335899360272634 1107 13 0.1550346330697039 1107 14 0.35517527342109645 1107 15 0.304282066383017 1107 16 0.42747452338605996 1107 17 0.3523262449121347 1107 18 0.2347768904781944 1107 19 0.504328594877741 1107 20 0.3869583267078127 1107 21 0.269815284076671 1107 22 0.511593156933408 1107 23 0.402432848299604 1107 24 0.16695424812962756 1107 25 0.25521651145704993 1108 1 0.3816216692338017 1108 2 0.1982635352267863 1108 3 0.5180148474159303 1108 4 0.3991575875824509 1108 5 0.41003753337452764 1108 6 0.32373942569865477 1108 7 0.26280157848137975 1108 8 0.1465925676244879 1108 9 0.16875086419871366 1108 10 0.24560771351852395 1108 11 0.190781180426157 1108 12 0.1706999252087371 1108 13 0.17641226967440815 1108 14 0.1557914748025766 1108 15 0.38342483514669823 1108 16 0.22894239629214042 1108 17 0.16030293227782977 1108 18 0.2042202788499452 1108 19 0.0950881632369871 1108 20 0.07684087779788484 1108 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-0.6557316107648035 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16812.0 3 19.0 4 274.0 5 1822.0 6 2525.0 7 1789.0 8 1433.0 9 1226.0 10 1168.0 11 1010.0 12 942.0 13 860.0 14 928.0 15 902.0 16 940.0 17 1022.0 18 1099.0 19 1320.0 20 1719.0 21 2224.0 22 2800.0 23 3417.0 24 4203.0 25 5060.0 26 6058.0 27 6640.0 28 7545.0 29 9316.0 30 12148.0 31 15817.0 32 20576.0 33 27440.0 34 37596.0 35 52228.0 36 77220.0 37 135069.0 38 323370.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.78467861729765 20.225019443217533 11.574028698316363 27.416273241168454 2 13.856922287746507 21.479604240678487 40.432188730304 24.231284741271004 3 17.57957393563384 25.73374855513748 29.835568605060736 26.85110890416794 4 11.57326355912055 18.524112660942844 38.68502053940247 31.217603240534135 5 12.633280017088321 38.74052449961984 35.60677531652812 13.019420166763721 6 30.08349652044676 38.85618059232138 18.65589100163931 12.404431885592544 7 27.383960396291414 32.54309878927912 22.258774579058 17.814166235371463 8 24.691053488848173 37.66381889548523 20.508529962630156 17.136597653036443 9 25.857066202817176 16.53519178294692 20.613710516210574 36.99403149802533 10 13.827541925722494 30.726119184752896 34.476230435722364 20.97010845380224 11 33.05191849480357 24.12352416141345 24.265291124498095 18.559266219284883 12 22.056389170716077 26.2954939010901 31.874527992432622 19.7735889357612 13 27.568455487563774 22.88499020384396 26.331889443926705 23.21466486466556 14 20.40374451551548 22.87838970067524 28.621412597245904 28.09645318656338 15 22.530333255775812 30.714431929287883 24.031007751937985 22.724227062998324 16 21.4415942390138 29.898719950364384 27.44834285191194 21.21134295870988 17 20.93404378942122 29.403702559447296 27.98482461727802 21.677429033853464 18 20.68581904914473 29.169495533568796 29.287735813073564 20.85694960421291 19 22.06075492952016 28.35505513408778 28.568649663016487 21.015540273375567 20 22.19531080148144 28.33696319661908 28.921555539451084 20.546170462448394 21 22.380771940175524 27.63830149764634 28.23530010147069 21.745626460707445 22 21.77074950065922 28.41582786315202 28.378703099790602 21.434719536398163 23 21.61693806767904 28.183961064719227 28.21587314503087 21.983227722570867 24 21.268039520073437 28.33407709999657 28.982997621218075 21.414885758711925 25 21.931251072811307 28.013757414318135 28.17103949852826 21.883952014342295 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 203.0 1 203.0 2 222.5 3 242.0 4 242.0 5 242.0 6 452.0 7 662.0 8 662.0 9 662.0 10 1135.5 11 1609.0 12 1609.0 13 1609.0 14 2884.0 15 4159.0 16 4159.0 17 4159.0 18 7447.5 19 10736.0 20 10736.0 21 10736.0 22 16722.5 23 22709.0 24 22709.0 25 22709.0 26 31605.0 27 40501.0 28 40501.0 29 40501.0 30 51482.5 31 62464.0 32 62464.0 33 62464.0 34 71907.5 35 81351.0 36 81351.0 37 81351.0 38 88671.0 39 95991.0 40 95991.0 41 95991.0 42 100186.5 43 104382.0 44 104382.0 45 104382.0 46 105616.0 47 106850.0 48 106850.0 49 106850.0 50 100785.0 51 94720.0 52 94720.0 53 94720.0 54 81486.0 55 68252.0 56 68252.0 57 68252.0 58 56540.0 59 44828.0 60 44828.0 61 44828.0 62 35194.0 63 25560.0 64 25560.0 65 25560.0 66 19181.5 67 12803.0 68 12803.0 69 12803.0 70 9101.0 71 5399.0 72 5399.0 73 5399.0 74 3735.5 75 2072.0 76 2072.0 77 2072.0 78 1396.5 79 721.0 80 721.0 81 721.0 82 480.0 83 239.0 84 239.0 85 239.0 86 156.5 87 74.0 88 74.0 89 74.0 90 41.5 91 9.0 92 9.0 93 9.0 94 4.5 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11645987410636753 2 0.11544275730194511 3 0.01652814807186439 4 0.0 5 0.004449886019348105 6 0.33971701267708954 7 0.018562381680709235 8 0.00940833044090742 9 0.009535470041460223 10 0.0034327692149256806 11 0.004449886019348105 12 3.8141880165840894E-4 13 5.085584022112119E-4 14 0.0 15 0.0030513504132672715 16 6.356980027640149E-4 17 0.0 18 3.8141880165840894E-4 19 0.0 20 7.628376033168179E-4 21 0.013095378856938707 22 0.0 23 1.2713960055280298E-4 24 0.0 25 0.006611259228745755 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 786537.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.65127720212172 #Duplication Level Percentage of deduplicated Percentage of total 1 92.92523884413126 81.45015869000318 2 5.702506629263137 9.996639786169602 3 0.7733215027638591 2.033478522153491 4 0.22042736958795783 0.7728296189875451 5 0.1091481008974837 0.4783485223925247 6 0.061207186137627864 0.32189328233466485 7 0.04044660399555408 0.24816375490891285 8 0.02482179314320496 0.17405294971590257 9 0.019177610299049283 0.151284783275661 >10 0.10542673747531328 1.6405452373621443 >50 0.010735192742329418 0.6454246019096976 >100 0.006382055823420948 1.109128384721524 >500 8.7028030488314E-4 0.5944846647238553 >1k 2.900934349610466E-4 0.3835672013412899 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1892 0.24054812424590324 No Hit GGTATCAACGCAGAGTACTTTTTTT 1125 0.14303205062190336 No Hit TATCAACGCAGAGTACTTTTTTTTT 956 0.12154545812847965 No Hit GTACATGGGAAGCAGTGGTATCAAC 940 0.11951122451963479 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.2713960055280298E-4 0.0 10 0.0 0.0 0.0 2.5427920110560596E-4 0.0 11 0.0 0.0 0.0 2.5427920110560596E-4 0.0 12 0.0 0.0 0.0 3.8141880165840894E-4 0.0 13 0.0 0.0 0.0 3.8141880165840894E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGTGA 95 3.110472E-10 14.979019 7 TAGGACC 170 0.0 11.765258 4 GTAGAAC 130 2.4974725E-9 11.723659 3 CGTGAAA 115 7.197377E-8 11.548306 9 AGGACGT 150 1.6734703E-10 11.429108 5 CTGGACC 100 1.8672035E-6 11.429108 4 GGACGTG 145 1.1623342E-9 11.16637 6 TTAGGAC 205 0.0 11.1517725 3 GTCCTAA 155 2.910383E-10 11.07598 1 ACGTGAA 120 1.2985765E-7 11.066423 8 TAGGACG 165 7.6397555E-11 10.967325 4 TAAGTAG 70 0.0014586095 10.884864 5 GGCGAGG 115 8.938914E-7 10.725472 19 GGTATCA 610 0.0 10.16307 1 CTTGAAC 85 6.4250286E-4 10.0857935 3 TAGGACA 180 3.7471182E-10 10.053382 4 GTATTAA 115 9.770221E-6 9.952331 1 GTAGGAC 270 0.0 9.878267 3 GACAGGT 125 2.6092384E-6 9.86618 7 ATAGGAC 90 0.0010853045 9.525473 3 >>END_MODULE