FastQCFastQC Report
Thu 26 May 2016
SRR1926967_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926967_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467733
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9120.19498303519315507No Hit
GGTATCAACGCAGAGTACTTTTTTT5650.12079541105716296No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG5210.11138833479784407No Hit
CACACACACACACACACACACACAC4930.10540201354191386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG402.799156E-416.5911219
AACTTAT400.005204841514.2774657
TAGGACC1600.013.09470754
AGGACCT1900.013.0250555
GTAGGAC2400.012.7238723
GGACCTG2000.012.3738026
GACGTGG853.8429607E-612.3178137
GTCATAG550.002949299512.1520571
TAGGGGT550.003006921412.120724
GGACGTG1209.655196E-911.8978866
TATTAGG657.707863E-411.7451132
ACAGTCC657.9407747E-411.703538
GGCGAGA1406.9667294E-1011.51220619
GGAATAT2800.011.50851312
TGTAGGA2750.011.4514872
TTAACCT752.0211065E-411.4281084
TAGGACG1251.7524144E-811.4281074
GGGTGTT752.0312195E-411.4219727
TGAACTT752.0312195E-411.4219725
GTCCTAC2850.011.390651
Produced by FastQC (version 0.11.5)