FastQCFastQC Report
Thu 26 May 2016
SRR1926967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926967_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467733
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15460.3305304522024317No Hit
GGTATCAACGCAGAGTACTTTTTTT10520.22491464147280604No Hit
TATCAACGCAGAGTACTTTTTTTTT8120.1736033164219758No Hit
CACACACACACACACACACACACAC7210.15414777234020263No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG5820.12442996324826343No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5500.11758845324148605No Hit
GTACATGGGAAGCAGTGGTATCAAC4850.10369163604021953No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAA400.005121395514.3122781
GAACCAT400.005169115514.2922946
GTGCTAG903.4158802E-813.7821941
TCTAGGA751.410267E-512.7206572
TCTACAC701.0569953E-412.2518533
GTCATAT550.00296445512.1437521
GCCTAAG550.00296445512.1437521
CTATACC959.981959E-712.0356154
GTCCAGG802.7315675E-511.9268981
TAGGACG1603.6379788E-1211.9102454
GTCCTAC2500.011.8314841
CACTGTT1157.134622E-811.5523897
AGGACGT1655.456968E-1211.5493285
GTCTAGG751.9857683E-411.4498221
TGTAGGA3350.011.3916332
GTACACT855.1463478E-511.2096414
TAAGGTT600.005737213411.1162294
GTAGGAC2750.011.0885473
TAGGACC1552.928573E-1011.06500154
GGACGTG1657.4578566E-1110.9718626
Produced by FastQC (version 0.11.5)