##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926967_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 467733 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.014675038964537 31.0 30.0 33.0 26.0 34.0 2 30.310136338466602 31.0 30.0 34.0 26.0 34.0 3 30.354266215982197 31.0 30.0 34.0 26.0 34.0 4 33.94371147641924 35.0 35.0 37.0 30.0 37.0 5 33.92095276578732 37.0 35.0 37.0 30.0 37.0 6 33.88645231360627 37.0 35.0 37.0 30.0 37.0 7 33.890668394147944 37.0 35.0 37.0 30.0 37.0 8 33.908248936893486 37.0 35.0 37.0 30.0 37.0 9 35.46724520185661 39.0 35.0 39.0 30.0 39.0 10 35.35610487179652 39.0 35.0 39.0 30.0 39.0 11 35.399437285801945 39.0 35.0 39.0 30.0 39.0 12 35.2970840201568 39.0 35.0 39.0 30.0 39.0 13 35.321931529312664 39.0 35.0 39.0 30.0 39.0 14 36.40550698796108 40.0 36.0 41.0 30.0 41.0 15 36.39609991170176 40.0 36.0 41.0 30.0 41.0 16 36.371870276418385 40.0 36.0 41.0 30.0 41.0 17 36.360511231835254 40.0 36.0 41.0 30.0 41.0 18 36.33269835568583 40.0 36.0 41.0 30.0 41.0 19 36.42473590702388 40.0 37.0 41.0 30.0 41.0 20 36.391736311100566 40.0 37.0 41.0 30.0 41.0 21 36.34816016830115 40.0 37.0 41.0 30.0 41.0 22 36.2636717956612 40.0 37.0 41.0 29.0 41.0 23 36.19650740914154 40.0 37.0 41.0 29.0 41.0 24 36.12766471469834 40.0 37.0 41.0 29.0 41.0 25 36.01777296021448 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38940407865550597 1101 2 0.4312131464227811 1101 3 0.5021518391097182 1101 4 0.3783708781978987 1101 5 0.35509818213139965 1101 6 0.4173109054566311 1101 7 0.5914191581212194 1101 8 0.24896265180970545 1101 9 0.3448964292257628 1101 10 0.4272964392715295 1101 11 0.40693046257062804 1101 12 0.25118004321640797 1101 13 0.620363881840106 1101 14 0.5594451387112471 1101 15 0.37594854130321664 1101 16 0.6351031301751036 1101 17 0.47036279591313956 1101 18 0.30509671424374574 1101 19 0.4517710824452692 1101 20 0.40351951914736617 1101 21 0.6458211203952473 1101 22 0.4605647237486252 1101 23 0.5174241658411702 1101 24 0.6804892871338595 1101 25 0.767241636768361 1102 1 -0.4602331385873448 1102 2 -0.44888562804219134 1102 3 -0.5250131711538835 1102 4 -0.6536109115303717 1102 5 -0.6231206256648747 1102 6 -0.818721450294035 1102 7 -0.5299352308388023 1102 8 -0.6570532415301287 1102 9 -0.30118612671594747 1102 10 -0.5787153892755086 1102 11 -0.6334369946982363 1102 12 -0.5326766321895633 1102 13 -0.5848614093006788 1102 14 -0.5821844367211426 1102 15 -0.5480540601641763 1102 16 -0.5822454054832917 1102 17 -0.6168981434604746 1102 18 -0.5215581645238103 1102 19 -0.7235739168433923 1102 20 -0.5427764384161975 1102 21 -0.8313022257421849 1102 22 -0.9236598154054931 1102 23 -0.7347698060711068 1102 24 -0.7294592932372552 1102 25 -0.5854360454867873 1103 1 -0.4704201147317697 1103 2 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13 -0.1232557760212245 1104 14 -0.3057103147534548 1104 15 -0.1316397639215552 1104 16 0.03366848510871279 1104 17 -0.21096263881251787 1104 18 -0.3264433127565951 1104 19 -0.1614288611361019 1104 20 -0.09211408217444017 1104 21 -0.2074239580870909 1104 22 -0.06076353190834993 1104 23 -0.19893320585682517 1104 24 -0.23031084988280526 1104 25 -0.16387525053543328 1105 1 0.12306822952415075 1105 2 -0.005792855835494493 1105 3 0.13389051306625888 1105 4 0.16418732398938118 1105 5 0.3055329991912785 1105 6 0.03438465597663054 1105 7 -0.04495888510296453 1105 8 -0.10564403189307114 1105 9 0.3508313354280972 1105 10 0.2194835987968986 1105 11 0.2427023371372954 1105 12 0.25045303701605093 1105 13 0.3040092137471575 1105 14 0.23886511850562897 1105 15 0.23706658571596506 1105 16 0.391835366065834 1105 17 0.339848344931589 1105 18 0.2916005473690788 1105 19 0.2677315428164988 1105 20 0.36585782464844385 1105 21 0.42787179292550803 1105 22 0.30347591805935537 1105 23 0.29989876024302475 1105 24 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-0.6476581924546991 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10148.0 3 10.0 4 152.0 5 1110.0 6 1581.0 7 1110.0 8 884.0 9 808.0 10 694.0 11 626.0 12 557.0 13 548.0 14 499.0 15 540.0 16 559.0 17 554.0 18 631.0 19 752.0 20 1064.0 21 1393.0 22 1740.0 23 2093.0 24 2583.0 25 2978.0 26 3546.0 27 3999.0 28 4698.0 29 5588.0 30 7433.0 31 9752.0 32 12347.0 33 16443.0 34 22420.0 35 31497.0 36 46660.0 37 80663.0 38 189073.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.25852227123585 19.383712493338212 10.760738586138775 27.59702664928716 2 13.963731623430405 20.92697741412853 40.93536712474772 24.17392383769334 3 18.873234015528475 24.6505451645116 28.91478155998948 27.56143925997045 4 11.25022181458225 17.525596868298795 38.71610512835314 32.50807618876581 5 12.294557964212943 38.97962630769855 35.5640555813312 13.161760146757292 6 30.916986159711477 38.65089905020993 18.146847370645546 12.285267419433044 7 28.381476190883507 31.976366233566278 21.09867503784995 18.543482537700264 8 24.71524759080325 38.59098072001146 19.71639151825901 16.97738017092628 9 25.92909360320043 16.290382242824794 19.865422814831287 37.91510133914349 10 13.383606220895675 31.177172374570784 34.65771817820292 20.78150322633062 11 34.14894367627596 23.830090206047807 23.16514398972006 18.85582212795618 12 21.954191814560872 26.589956235715672 31.921416705684653 19.5344352440388 13 28.265605657952968 22.512084937709922 25.82659691147262 23.395712492864487 14 19.9278220694285 23.169842623890126 28.606705107401016 28.295630199280357 15 22.71391980706488 31.04921299404347 23.71686600159924 22.52000119729241 16 21.00433581623665 30.38107292210069 27.742168592270787 20.872422669391874 17 20.669056919225284 29.563661319599 28.057032537793997 21.710249223381716 18 20.114339224896362 29.502000081243278 29.669617793133234 20.71404290072713 19 21.78811798209231 28.35598163050636 28.84194367714845 21.013956710252877 20 21.89301924832542 28.491070684092712 29.359736086494532 20.25617398108734 21 22.31064963500528 27.695123567924938 28.406082817665144 21.588143979404634 22 21.302110392039904 28.96011185868861 28.724293560642504 21.013484188628983 23 21.3600494299098 28.368748837477792 28.475006039770552 21.796195692841856 24 20.846722382213784 28.236408378284196 29.728498951324795 21.18837028817723 25 21.485459914005993 28.26299371187517 28.826380618635174 21.42516575548367 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 187.0 1 187.0 2 186.5 3 186.0 4 186.0 5 186.0 6 332.5 7 479.0 8 479.0 9 479.0 10 773.0 11 1067.0 12 1067.0 13 1067.0 14 1797.5 15 2528.0 16 2528.0 17 2528.0 18 4243.0 19 5958.0 20 5958.0 21 5958.0 22 9482.5 23 13007.0 24 13007.0 25 13007.0 26 17828.0 27 22649.0 28 22649.0 29 22649.0 30 29387.5 31 36126.0 32 36126.0 33 36126.0 34 41815.5 35 47505.0 36 47505.0 37 47505.0 38 51902.0 39 56299.0 40 56299.0 41 56299.0 42 59588.0 43 62877.0 44 62877.0 45 62877.0 46 63848.0 47 64819.0 48 64819.0 49 64819.0 50 61844.0 51 58869.0 52 58869.0 53 58869.0 54 50502.0 55 42135.0 56 42135.0 57 42135.0 58 34378.5 59 26622.0 60 26622.0 61 26622.0 62 20764.0 63 14906.0 64 14906.0 65 14906.0 66 11003.5 67 7101.0 68 7101.0 69 7101.0 70 4989.0 71 2877.0 72 2877.0 73 2877.0 74 1976.5 75 1076.0 76 1076.0 77 1076.0 78 706.5 79 337.0 80 337.0 81 337.0 82 218.0 83 99.0 84 99.0 85 99.0 86 59.0 87 19.0 88 19.0 89 19.0 90 11.5 91 4.0 92 4.0 93 4.0 94 2.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1103193488592851 2 0.10561581072962567 3 0.016248586266096255 4 0.0 5 0.004703538129659442 6 0.3484894159702223 7 0.020738327208043906 8 0.00983467063474247 9 0.00983467063474247 10 0.004062146566524064 11 0.005558726880506614 12 0.0 13 4.2759437542358566E-4 14 0.0 15 0.002351769064829721 16 2.1379718771179283E-4 17 0.0 18 4.2759437542358566E-4 19 2.1379718771179283E-4 20 8.551887508471713E-4 21 0.011758845324148606 22 0.0 23 0.0 24 0.0 25 0.005558726880506614 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 467733.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.82418792018395 #Duplication Level Percentage of deduplicated Percentage of total 1 95.36245626673794 85.6585519223379 2 3.753019404203987 6.742238404626313 3 0.41165236318167164 1.1092901768465488 4 0.14013667478719366 0.5035065204237835 5 0.08079531660391923 0.3628686850850599 6 0.053514116172826226 0.28841172164942985 7 0.03617674281733168 0.2274682581614916 8 0.025358896639521816 0.1822273837757543 9 0.01768520110059754 0.1429702944359685 >10 0.09991763357147111 1.7262553160166043 >50 0.010000867967308112 0.605510294770702 >100 0.007857821413782294 1.3250211930954743 >500 9.524632016704389E-4 0.5700050814526457 >1k 4.7623160083521944E-4 0.5556747473223166 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1546 0.3305304522024317 No Hit GGTATCAACGCAGAGTACTTTTTTT 1052 0.22491464147280604 No Hit TATCAACGCAGAGTACTTTTTTTTT 812 0.1736033164219758 No Hit CACACACACACACACACACACACAC 721 0.15414777234020263 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 582 0.12442996324826343 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 550 0.11758845324148605 No Hit GTACATGGGAAGCAGTGGTATCAAC 485 0.10369163604021953 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.1379718771179283E-4 0.0 10 0.0 0.0 0.0 4.2759437542358566E-4 0.0 11 0.0 0.0 0.0 4.2759437542358566E-4 0.0 12 0.0 0.0 0.0 4.2759437542358566E-4 0.0 13 0.0 0.0 0.0 4.2759437542358566E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTTAA 40 0.0051213955 14.312278 1 GAACCAT 40 0.0051691155 14.292294 6 GTGCTAG 90 3.4158802E-8 13.782194 1 TCTAGGA 75 1.410267E-5 12.720657 2 TCTACAC 70 1.0569953E-4 12.251853 3 GTCATAT 55 0.002964455 12.143752 1 GCCTAAG 55 0.002964455 12.143752 1 CTATACC 95 9.981959E-7 12.035615 4 GTCCAGG 80 2.7315675E-5 11.926898 1 TAGGACG 160 3.6379788E-12 11.910245 4 GTCCTAC 250 0.0 11.831484 1 CACTGTT 115 7.134622E-8 11.552389 7 AGGACGT 165 5.456968E-12 11.549328 5 GTCTAGG 75 1.9857683E-4 11.449822 1 TGTAGGA 335 0.0 11.391633 2 GTACACT 85 5.1463478E-5 11.209641 4 TAAGGTT 60 0.0057372134 11.116229 4 GTAGGAC 275 0.0 11.088547 3 TAGGACC 155 2.928573E-10 11.0650015 4 GGACGTG 165 7.4578566E-11 10.971862 6 >>END_MODULE