FastQCFastQC Report
Thu 26 May 2016
SRR1926966_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926966_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549627
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9890.17994021399967614No Hit
GTATCAACGCAGAGTACTTTTTTTT8210.1493740300239981No Hit
CTGTAGGACGTGGAATATGGCAAGA8150.1482823805962953No Hit
GTCCTACAGTGTGCATTTCTCATTT7440.13536452903514565No Hit
GTCCTAAAGTGTGTATTTCTCATTT6870.12499385947196918No Hit
GTACATGGGAAGCAGTGGTATCAAC6010.10934688434156255No Hit
CTTTAGGACGTGAAATATGGCGAGG5830.10607193605845419No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGC551.9010466E-413.8650923
GGCGAGG1500.013.27229319
GTAACCC655.2696196E-513.1985013
TGTAGGA5000.012.9638612
TGGCGAG4200.012.4145618
GTGCTAG701.0473221E-412.2647281
GTAGGAC4750.012.2414173
TAGGACC1950.012.2130124
CTACTAG550.002970925512.14084151
CTATTGG550.002970925512.14084151
TAGGACG4450.011.9879654
AGGACGT4700.011.9518015
CTGTAGG5350.011.946331
GGACGTG4600.011.797676
AGACTTA657.862369E-411.7180636
GACGTGG2450.011.65827757
CTATTAT1156.6442226E-811.6129791
TATGGCG4550.011.45959316
ATAATAC752.0050077E-411.4387013
CTAGACT752.0169724E-411.4313794
Produced by FastQC (version 0.11.5)