##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926966_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 549627 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.85931186058909 31.0 28.0 31.0 16.0 34.0 2 27.861859042587064 31.0 28.0 31.0 16.0 34.0 3 27.42955859155391 31.0 27.0 31.0 12.0 34.0 4 31.231606161997135 35.0 32.0 37.0 17.0 37.0 5 31.075593084036992 35.0 32.0 37.0 17.0 37.0 6 30.68720968948032 35.0 31.0 37.0 11.0 37.0 7 30.52167197026347 35.0 30.0 37.0 11.0 37.0 8 30.69234408062195 35.0 32.0 37.0 11.0 37.0 9 32.827939675452626 37.0 32.0 39.0 17.0 39.0 10 33.018548943192386 37.0 33.0 39.0 17.0 39.0 11 33.16489364605451 37.0 33.0 39.0 17.0 39.0 12 33.06307368451695 38.0 33.0 39.0 17.0 39.0 13 33.14327170972314 38.0 33.0 39.0 17.0 39.0 14 34.12516670396469 39.0 34.0 40.0 17.0 41.0 15 33.73355748534915 39.0 33.0 40.0 10.0 41.0 16 33.81085354249336 39.0 33.0 40.0 11.0 41.0 17 34.024376531720605 39.0 34.0 40.0 16.0 41.0 18 34.057875613825374 39.0 34.0 41.0 11.0 41.0 19 33.46171312544689 39.0 33.0 40.0 10.0 41.0 20 33.75434430986833 39.0 33.0 41.0 10.0 41.0 21 33.73357204067486 39.0 34.0 41.0 8.0 41.0 22 33.74270914638473 39.0 34.0 41.0 2.0 41.0 23 33.73004055477624 39.0 34.0 41.0 2.0 41.0 24 32.460019249418245 38.0 32.0 40.0 2.0 41.0 25 33.025126130994295 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.5082543456337163 1101 2 -0.46978544319321003 1101 3 -1.3495803589824718 1101 4 -0.9770967880787609 1101 5 -0.9231886937101201 1101 6 -2.670000091409534 1101 7 -1.1692218161406522 1101 8 -0.4261088947731544 1101 9 -0.36843473560448814 1101 10 -8.915332575573846 1101 11 -4.192600696583092 1101 12 -1.8693867873265617 1101 13 -1.0790260012433137 1101 14 -0.7244026129276477 1101 15 -2.055305154860811 1101 16 -0.5838193925875643 1101 17 -0.49995718811667444 1101 18 -0.25233755882631925 1101 19 -0.9824839559715031 1101 20 -0.2678966423424285 1101 21 -0.28676812995857404 1101 22 -0.28353397973599215 1101 23 -0.3251084677711731 1101 24 -0.23402835123981447 1101 25 -0.12053251917892993 1102 1 -0.15210821552925324 1102 2 -0.2356923757668099 1102 3 -0.7943512516700331 1102 4 -0.19049006918037392 1102 5 -0.1333471694935966 1102 6 -0.9916792224636666 1102 7 -0.12188088546638909 1102 8 0.5356165325772757 1102 9 0.2868804353356822 1102 10 0.27528648900355535 1102 11 -0.0026196899354218317 1102 12 0.05467541590230951 1102 13 -0.30946581605812895 1102 14 -0.4215328360995372 1102 15 0.07531569034336627 1102 16 0.13013775366124491 1102 17 -0.04876416817365481 1102 18 -0.09954791020333431 1102 19 0.33280272494017993 1102 20 0.16081299678387495 1102 21 0.24385568295690518 1102 22 0.3160624115270707 1102 23 -0.20618930822701742 1102 24 -3.85720617650097 1102 25 -2.0031090861304968 1103 1 0.4321413286072975 1103 2 0.26396385835269953 1103 3 0.4735768863146035 1103 4 0.7240317973188937 1103 5 0.4672267700633945 1103 6 1.1684678495478131 1103 7 0.7299679771169352 1103 8 1.3104080310975874 1103 9 1.193794613436296 1103 10 1.1299243391216294 1103 11 0.9156596871010194 1103 12 0.7080368483597823 1103 13 0.9646710326549908 1103 14 0.9886695373796712 1103 15 1.3172909989853423 1103 16 1.2332525714653286 1103 17 0.8954202099090338 1103 18 0.9175148345430273 1103 19 -0.6528398183294826 1103 20 0.5983552202018032 1103 21 0.7863571028594549 1103 22 0.9360367038177415 1103 23 0.8153244573545493 1103 24 2.0818067670317433 1103 25 1.3951332239762237 1104 1 -0.10490996549928866 1104 2 -0.11933734759948322 1104 3 -1.2162019049122712 1104 4 -0.4631288082430629 1104 5 -0.24643598646590803 1104 6 -1.747144882298258 1104 7 -0.39214937401966665 1104 8 0.08823249805731948 1104 9 0.3114219671587648 1104 10 0.4372276372715618 1104 11 0.12928130491056322 1104 12 0.1831244150632827 1104 13 0.029135406523678853 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-0.09735859880105835 2215 1 0.19409316435894297 2215 2 -4.5678669594195753E-4 2215 3 0.4291408866329931 2215 4 0.31988527611897055 2215 5 0.6537586001148235 2215 6 1.401973815515987 2215 7 0.8392413537753249 2215 8 -0.38710056415891003 2215 9 -0.024242458501952058 2215 10 -0.3915477542181094 2215 11 -0.3870088864843879 2215 12 -0.7586875051665132 2215 13 -0.3173445325264552 2215 14 -0.1845887516828526 2215 15 -0.24474505615781794 2215 16 0.10157118780234953 2215 17 -0.4244132000262937 2215 18 -1.0397335536056644 2215 19 0.017206322599342627 2215 20 -3.8158231657130166 2215 21 -2.209808090395807 2215 22 -1.1422398285001591 2215 23 -1.074796197194452 2215 24 -1.1643321076636646 2215 25 -0.5663312690769615 2216 1 -1.1617859021644321 2216 2 -1.7152681484074606 2216 3 -0.8062412176987017 2216 4 -1.3391767872183244 2216 5 -1.2386168687230281 2216 6 -2.669556428528793 2216 7 -10.274238164445592 2216 8 -12.035822691882085 2216 9 -7.204916730958129 2216 10 -4.671428234668667 2216 11 -3.6769837631809246 2216 12 -3.3054455551806328 2216 13 -3.0868210236146787 2216 14 -3.1076708757051392 2216 15 -3.973536741506827 2216 16 -3.1826056385004904 2216 17 -3.4112299896998763 2216 18 -3.048102057191489 2216 19 -2.4350819382464977 2216 20 -3.7657509273192 2216 21 -6.095285919495662 2216 22 -4.681120453664747 2216 23 -4.691598207058732 2216 24 -4.612206590785501 2216 25 -4.242507544992041 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35125.0 3 3.0 4 227.0 5 1447.0 6 1885.0 7 1447.0 8 1220.0 9 1126.0 10 1033.0 11 999.0 12 1052.0 13 1095.0 14 1076.0 15 1068.0 16 1149.0 17 1307.0 18 1425.0 19 1826.0 20 2351.0 21 3039.0 22 3779.0 23 4484.0 24 5285.0 25 5945.0 26 6729.0 27 7583.0 28 8339.0 29 9960.0 30 11753.0 31 14254.0 32 18509.0 33 24272.0 34 32641.0 35 45522.0 36 66176.0 37 103166.0 38 121330.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.90015391236478 19.19254363889679 12.556805333683466 25.350497115054964 2 13.647427932179179 20.763658359721457 43.13283217152706 22.4560815365723 3 19.50025872561238 24.88739969827492 29.28118008031426 26.331161495798444 4 11.00809689324737 17.68298122984653 39.909836259588545 31.399085617317557 5 11.870332086129835 38.92621602341054 36.492895307700145 12.710556582759475 6 29.851830013976237 39.49616381318425 18.81969921965991 11.832306953179595 7 26.986177637881482 31.864969663792706 22.773413621641346 18.375439076684458 8 23.400959378757552 38.51984169703118 21.99163234883581 16.087566575375458 9 25.71248673840213 16.169959546439706 20.994178545236835 37.12337516992133 10 13.179596851253583 30.651499294717205 36.00345816080448 20.165445693224733 11 33.46392859417507 23.74225481543487 24.73018097118473 18.06363561920533 12 21.987010694613023 26.091434664958534 33.035017260235584 18.886537380192856 13 28.09424678274035 23.14152303033832 26.127860595120247 22.636369591801085 14 19.21004503889723 23.712660934443385 28.93353350620991 28.14376052044948 15 22.257787450549372 32.36027353886355 23.764689612001355 21.617249398585724 16 20.344994504254654 30.149986533604118 29.25811077384462 20.24690818829661 17 20.296585571797984 30.098212807153867 28.88115285858047 20.724048762467675 18 19.89500309349638 29.52596717254431 30.759726316555664 19.819303417403646 19 22.29689521698541 28.393268875555727 28.50518737704888 20.804648530409985 20 21.948716268963896 28.91902367655328 29.523357190040233 19.608902864442587 21 21.63288405092959 28.252566223207282 28.1948819291666 21.91966779669653 22 21.028371569107364 29.859206702467024 28.882188201168635 20.230233527256978 23 20.73132117497625 29.338193279298885 28.805370446530283 21.125115099194577 24 21.569169795046395 28.5097145061588 29.653582366310673 20.267533332484135 25 21.227676865462513 28.809262986268564 29.180117261283034 20.78294288698589 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 171.0 1 171.0 2 240.5 3 310.0 4 310.0 5 310.0 6 462.5 7 615.0 8 615.0 9 615.0 10 986.5 11 1358.0 12 1358.0 13 1358.0 14 2268.5 15 3179.0 16 3179.0 17 3179.0 18 5503.5 19 7828.0 20 7828.0 21 7828.0 22 12274.0 23 16720.0 24 16720.0 25 16720.0 26 23420.5 27 30121.0 28 30121.0 29 30121.0 30 39042.5 31 47964.0 32 47964.0 33 47964.0 34 54939.0 35 61914.0 36 61914.0 37 61914.0 38 66480.5 39 71047.0 40 71047.0 41 71047.0 42 72983.5 43 74920.0 44 74920.0 45 74920.0 46 75071.5 47 75223.0 48 75223.0 49 75223.0 50 69511.0 51 63799.0 52 63799.0 53 63799.0 54 53518.5 55 43238.0 56 43238.0 57 43238.0 58 34890.5 59 26543.0 60 26543.0 61 26543.0 62 20322.0 63 14101.0 64 14101.0 65 14101.0 66 10344.0 67 6587.0 68 6587.0 69 6587.0 70 4585.0 71 2583.0 72 2583.0 73 2583.0 74 1754.0 75 925.0 76 925.0 77 925.0 78 633.5 79 342.0 80 342.0 81 342.0 82 217.0 83 92.0 84 92.0 85 92.0 86 62.0 87 32.0 88 32.0 89 32.0 90 20.0 91 8.0 92 8.0 93 8.0 94 7.0 95 6.0 96 6.0 97 6.0 98 3.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22979220453143678 2 0.1399130683172406 3 0.14246025031521375 4 0.07350439479865437 5 0.023834345838177527 6 0.022378813267907145 7 0.29147039719664425 8 0.28455661748785993 9 0.019649689698650174 10 0.03675219739932718 11 0.01673862455810941 12 0.017830273985812196 13 0.01655668298682561 14 0.018558040270947387 15 0.016192799844258015 16 0.02019551441250157 17 0.01928580655608258 18 0.01582891670169042 19 0.02183298855405575 20 0.0174663908432446 21 0.015283091987839025 22 0.017830273985812196 23 0.01837609869966359 24 0.01601085827297422 25 0.015465033559122824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 549627.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.69761665434933 #Duplication Level Percentage of deduplicated Percentage of total 1 94.91710605571339 84.18921086869885 2 4.020245645762051 7.13172414288239 3 0.5225595491398022 1.3904935970601537 4 0.15744905225838232 0.5586142271921845 5 0.09103672578627614 0.40373703026291197 6 0.06102702886815599 0.3247771207260966 7 0.03449304496752424 0.21416156158394892 8 0.030703935470103985 0.217869271848573 9 0.017970038888856482 0.14345096585647876 >10 0.12562918038538246 2.1336846756717387 >50 0.00965707579704304 0.5569456447388251 >100 0.010684384442049954 1.780361616265907 >500 0.001438282521003449 0.9549692772119183 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 989 0.17994021399967614 No Hit GTATCAACGCAGAGTACTTTTTTTT 821 0.1493740300239981 No Hit CTGTAGGACGTGGAATATGGCAAGA 815 0.1482823805962953 No Hit GTCCTACAGTGTGCATTTCTCATTT 744 0.13536452903514565 No Hit GTCCTAAAGTGTGTATTTCTCATTT 687 0.12499385947196918 No Hit GTACATGGGAAGCAGTGGTATCAAC 601 0.10934688434156255 No Hit CTTTAGGACGTGAAATATGGCGAGG 583 0.10607193605845419 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.638831425675958E-4 0.0 10 0.0 0.0 0.0 3.638831425675958E-4 0.0 11 0.0 0.0 0.0 3.638831425675958E-4 0.0 12 0.0 0.0 0.0 3.638831425675958E-4 1.819415712837979E-4 13 0.0 0.0 0.0 3.638831425675958E-4 1.819415712837979E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAGGC 55 1.9010466E-4 13.865092 3 GGCGAGG 150 0.0 13.272293 19 GTAACCC 65 5.2696196E-5 13.198501 3 TGTAGGA 500 0.0 12.963861 2 TGGCGAG 420 0.0 12.41456 18 GTGCTAG 70 1.0473221E-4 12.264728 1 GTAGGAC 475 0.0 12.241417 3 TAGGACC 195 0.0 12.213012 4 CTACTAG 55 0.0029709255 12.1408415 1 CTATTGG 55 0.0029709255 12.1408415 1 TAGGACG 445 0.0 11.987965 4 AGGACGT 470 0.0 11.951801 5 CTGTAGG 535 0.0 11.94633 1 GGACGTG 460 0.0 11.79767 6 AGACTTA 65 7.862369E-4 11.718063 6 GACGTGG 245 0.0 11.6582775 7 CTATTAT 115 6.6442226E-8 11.612979 1 TATGGCG 455 0.0 11.459593 16 ATAATAC 75 2.0050077E-4 11.438701 3 CTAGACT 75 2.0169724E-4 11.431379 4 >>END_MODULE