FastQCFastQC Report
Thu 26 May 2016
SRR1926966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926966_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549627
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14260.2594486806506958No Hit
GTCCTACAGTGGACATTTCTAAATT10180.18521651956690627No Hit
CTGTAGGACGTGGAATATGGCAAGA9610.17484585000372982No Hit
GGTATCAACGCAGAGTACTTTTTTT8130.14791849745372773No Hit
GTCCTACAGTGTGCATTTCTCATTT7780.14155054245879478No Hit
TATCAACGCAGAGTACTTTTTTTTT6860.12481191790068538No Hit
GTACATGGGAAGCAGTGGTATCAAC6710.1220827943314284No Hit
GTCCTAAAGTGTGTATTTCTCATTT6630.12062726176115801No Hit
CACACACACACACACACACACACAC6570.11953561233345522No Hit
CTTTAGGACGTGAAATATGGCGAGG6450.11735231347804966No Hit
CTGTAGGACCTGGAATATGGCGAGA6060.11025659219798155No Hit
CCCATGTACTCTGCGTTGATACCAC5730.10425252034561622No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG5580.10152339677635926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCCA453.579224E-516.8628512
GCGCCAC402.7896845E-416.59936713
GTCTTAC508.410717E-515.2598611
AATGCCG456.8272365E-414.75633618
ATGCCGG456.8272365E-414.75633619
GCAAATC456.8319484E-414.75499313
CGCCACC456.8319484E-414.75499314
GGCGAGG1650.013.79813319
AAGGCGT500.001466759513.3365186
TAGGACC1950.013.1899644
TGTAGGA5550.012.7165512
CCTCTAA603.9512708E-412.7165511
GTAGGAC5050.012.6397243
GTCTTAT959.876512E-712.0472591
GTGTAGC1603.6379788E-1211.9217661
CTGTAGG5800.011.8395471
GGACGTG4550.011.7244126
TAGGACG4800.011.7091464
AACAGTC658.10091E-411.6764057
TTTAGGA2950.011.6388782
Produced by FastQC (version 0.11.5)