FastQCFastQC Report
Thu 26 May 2016
SRR1926965_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926965_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610131
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT7830.12833309567945245No Hit
GTATCAACGCAGAGTACTTTTTTTT7240.11866304121573891No Hit
GTCCTACAGTGTGCATTTCTCATTT6910.11325436668518728No Hit
CTGTAGGACGTGGAATATGGCAAGA6740.1104680798058122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCATA456.510808E-414.8493961
ATAAGGC456.568073E-414.8322623
ACGTTTT400.005341433514.22283219
TAGGACC1900.014.0331134
ACGTGAA1301.8189894E-1213.8980098
GTGTAAG1204.7293724E-1113.5235571
GTAGGAC4400.013.4357173
GACGTGA1259.822543E-1112.9420377
TGGACTA604.027346E-412.68827155
GGACGTG3150.012.6882716
TGTAGGA4850.012.5833392
GTCCTAA1850.012.3840511
GAGTTAG550.002955737312.1495051
GTCTTAT550.002955737312.1495051
CGTGAAA1501.4551915E-1112.0123629
GTCCTAG1209.271389E-911.9325491
GACGTGG2000.011.8952557
TAGGACG3700.011.8387454
CTGTAGG4850.011.8095331
TTAGGAC1950.011.7354163
Produced by FastQC (version 0.11.5)