##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926965_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 610131 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.20158949471507 31.0 28.0 31.0 18.0 34.0 2 28.2031334254447 31.0 28.0 31.0 16.0 34.0 3 27.80018389493404 31.0 28.0 31.0 16.0 34.0 4 31.65202390961941 35.0 32.0 37.0 19.0 37.0 5 31.492141851504023 35.0 32.0 37.0 19.0 37.0 6 31.055081613620683 35.0 32.0 37.0 16.0 37.0 7 30.901162209427156 35.0 31.0 37.0 16.0 37.0 8 31.057236888471493 35.0 32.0 37.0 13.0 37.0 9 33.26237971845391 37.0 33.0 39.0 17.0 39.0 10 33.447697297793425 37.0 34.0 39.0 18.0 39.0 11 33.59861734611092 38.0 34.0 39.0 21.0 39.0 12 33.489327701755855 38.0 34.0 39.0 18.0 39.0 13 33.5737898910234 38.0 34.0 39.0 19.0 39.0 14 34.611526049323835 39.0 34.0 41.0 19.0 41.0 15 34.20343827800915 39.0 34.0 41.0 17.0 41.0 16 34.29016227662584 39.0 34.0 41.0 17.0 41.0 17 34.50726647228218 39.0 34.0 41.0 18.0 41.0 18 34.54574345509407 39.0 34.0 41.0 18.0 41.0 19 33.92164797395969 39.0 33.0 41.0 11.0 41.0 20 34.21498170065117 39.0 34.0 41.0 17.0 41.0 21 34.19484995845155 39.0 34.0 41.0 16.0 41.0 22 34.220993524341495 39.0 34.0 41.0 11.0 41.0 23 34.205013349592136 39.0 34.0 41.0 10.0 41.0 24 32.92049740137774 39.0 32.0 40.0 2.0 41.0 25 33.49189272467716 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.598974665545736 1101 2 -0.7043786541932491 1101 3 -1.3879529752473339 1101 4 -0.9189886720169334 1101 5 -0.9067341709437748 1101 6 -2.4632062654963036 1101 7 -1.0418675385712142 1101 8 -0.6566759233984314 1101 9 -0.6031632188726448 1101 10 -9.66670703694492 1101 11 -4.530018659354415 1101 12 -1.9479404217047502 1101 13 -1.125299045318684 1101 14 -1.0864420834176798 1101 15 -2.516673762752312 1101 16 -0.8882963763119633 1101 17 -0.8463098515013456 1101 18 -0.6709322223111087 1101 19 -1.6001842493463343 1101 20 -0.6453069661591329 1101 21 -0.7625678253790085 1101 22 -0.3767794432169893 1101 23 -0.6270672046631134 1101 24 -0.8945803732682691 1101 25 -0.744053778103833 1102 1 -0.36340788483277464 1102 2 -0.5923220519896617 1102 3 -0.6311138686262829 1102 4 -0.660774157301045 1102 5 -0.45963598834244124 1102 6 -1.1945562701462613 1102 7 -0.6355470244328991 1102 8 -0.045157737175028245 1102 9 -0.06916959175997306 1102 10 -0.23861493844416515 1102 11 -0.35114463400970664 1102 12 -0.09176601408469764 1102 13 -0.34710195570179536 1102 14 -0.5706469164353081 1102 15 -0.1819870474324219 1102 16 -0.1513590821948938 1102 17 -0.47183419524568393 1102 18 -0.5837700171550395 1102 19 -0.16514658470116927 1102 20 -0.19579570102842325 1102 21 -0.3735353588954027 1102 22 -0.357308363497161 1102 23 -0.8459284059181087 1102 24 -4.148147379137004 1102 25 -2.4467869734068834 1103 1 0.1809367908481221 1103 2 -0.05555700457572854 1103 3 0.06583178931380829 1103 4 -0.0653253567671328 1103 5 -0.24143167940750132 1103 6 0.5085902351085103 1103 7 0.3463625871860181 1103 8 0.581033722694869 1103 9 0.44240947912745554 1103 10 0.319197297992325 1103 11 0.16007385143364417 1103 12 0.0029225012077915835 1103 13 0.10900807297392134 1103 14 0.06619098146155977 1103 15 0.5685281619393763 1103 16 0.3670185522179139 1103 17 0.0889776231561541 1103 18 -0.050099892942519375 1103 19 -1.1551467558264648 1103 20 -0.003206404182257927 1103 21 -0.011164100122179832 1103 22 0.21650531039180265 1103 23 0.2024291579421842 1103 24 1.7205567378931406 1103 25 0.9210138465277424 1104 1 0.21745995804907636 1104 2 0.2774076039027591 1104 3 -0.9338843895808182 1104 4 0.15781867759219992 1104 5 0.07818120801134043 1104 6 -0.7379496338728764 1104 7 0.11695993357997736 1104 8 0.9888773736531284 1104 9 0.88638937877122 1104 10 0.9786677654359792 1104 11 0.7007203085821132 1104 12 0.810363223229281 1104 13 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-2.4312864718624674 2216 12 -2.293479456758565 2216 13 -1.92586337649605 2216 14 -1.963427335219336 2216 15 -2.985609779450332 2216 16 -2.04820094679323 2216 17 -2.3732551620552798 2216 18 -2.2141417807241055 2216 19 -1.6223838525107084 2216 20 -2.349591416558976 2216 21 -5.161568005758838 2216 22 -3.5622550017502483 2216 23 -3.4830102905324978 2216 24 -3.814442339751981 2216 25 -3.278690999910893 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34128.0 3 5.0 4 235.0 5 1479.0 6 1913.0 7 1543.0 8 1199.0 9 1205.0 10 1117.0 11 1081.0 12 1063.0 13 1018.0 14 1049.0 15 1114.0 16 1174.0 17 1331.0 18 1465.0 19 1953.0 20 2409.0 21 3153.0 22 3991.0 23 4663.0 24 5550.0 25 6437.0 26 7452.0 27 7921.0 28 8951.0 29 10536.0 30 12742.0 31 15620.0 32 20066.0 33 26612.0 34 36125.0 35 49947.0 36 73201.0 37 116535.0 38 144148.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74958928594898 18.424796614401934 12.06332882095496 25.762285278694126 2 14.492032935021113 20.0818616131727 41.79636021079201 23.629745241014177 3 19.801447213154574 23.95402004565828 28.76849020775994 27.476042533427208 4 11.489688362686193 16.781076386325946 38.70394701230705 33.025288238680815 5 12.464568336183167 38.17127531038055 36.097262683674955 13.266893669761318 6 31.564296183116003 38.25802411250316 18.003554249853273 12.174125454527571 7 28.174177807882263 31.134957221677855 21.99351584540618 18.697349125033703 8 24.591425231762713 37.4984587512515 21.116505253397737 16.79361076358806 9 25.93406197079658 15.940078393828166 20.067311806451507 38.05854782892375 10 13.707315793443364 29.836887549476458 35.18575602653611 21.270040630544067 11 34.57593387660223 23.155156218494422 23.842684804044517 18.426225100858836 12 22.541380672680805 25.41675751261871 31.894224164640733 20.147637650059753 13 28.480705550637687 22.213042195337856 25.640962591390448 23.665289662634013 14 20.108523700625405 22.559323284235376 28.026819452299573 29.305333562839646 15 22.88762409347829 31.153528385768432 23.240397099130526 22.718450421622755 16 21.636185392595316 29.051036734908 27.86823433749951 21.44454353499718 17 21.2146597201958 29.302555090344555 27.8594356047069 21.623349584752738 18 21.057014524113963 28.614471656666996 29.320022294351006 21.008491524868038 19 23.00482645105021 27.658318742499656 27.590610716557478 21.74624408989265 20 22.66613333945348 27.983121505163055 28.545737999727873 20.80500715565559 21 22.431432463579004 27.50242205570646 27.532912909270042 22.533232571444493 22 22.054428705150254 28.659085357757018 27.716640246291448 21.569845690801284 23 21.749600820980916 28.237776269586 27.9848262792581 22.027796630174983 24 22.415859178156747 27.747004601496016 28.46960504508876 21.367531175258474 25 22.01279306512267 27.98146939926363 28.11671131015806 21.889026225455645 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 174.0 1 174.0 2 237.0 3 300.0 4 300.0 5 300.0 6 467.5 7 635.0 8 635.0 9 635.0 10 919.0 11 1203.0 12 1203.0 13 1203.0 14 2068.5 15 2934.0 16 2934.0 17 2934.0 18 5174.0 19 7414.0 20 7414.0 21 7414.0 22 11663.0 23 15912.0 24 15912.0 25 15912.0 26 22174.0 27 28436.0 28 28436.0 29 28436.0 30 36717.0 31 44998.0 32 44998.0 33 44998.0 34 52630.0 35 60262.0 36 60262.0 37 60262.0 38 65812.0 39 71362.0 40 71362.0 41 71362.0 42 75213.5 43 79065.0 44 79065.0 45 79065.0 46 81058.0 47 83051.0 48 83051.0 49 83051.0 50 78793.0 51 74535.0 52 74535.0 53 74535.0 54 65468.0 55 56401.0 56 56401.0 57 56401.0 58 47557.0 59 38713.0 60 38713.0 61 38713.0 62 30992.5 63 23272.0 64 23272.0 65 23272.0 66 17820.5 67 12369.0 68 12369.0 69 12369.0 70 8960.0 71 5551.0 72 5551.0 73 5551.0 74 3920.0 75 2289.0 76 2289.0 77 2289.0 78 1569.5 79 850.0 80 850.0 81 850.0 82 566.5 83 283.0 84 283.0 85 283.0 86 188.0 87 93.0 88 93.0 89 93.0 90 55.5 91 18.0 92 18.0 93 18.0 94 13.0 95 8.0 96 8.0 97 8.0 98 5.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2351953924648969 2 0.13275837484081288 3 0.13472516557919528 4 0.07867162953529652 5 0.0252404811425743 6 0.025732178827169903 7 0.3079666497850462 8 0.3045247659928769 9 0.02196249657860361 10 0.04015531090864093 11 0.01966790738382413 12 0.020323504296618267 13 0.018192814330037322 14 0.020651302753015337 15 0.017537217417243182 16 0.022781992719596283 17 0.021798597350405076 18 0.018192814330037322 19 0.026059977283566974 20 0.020323504296618267 21 0.018356713558235854 22 0.019995705840221197 23 0.02114300043761094 24 0.018356713558235854 25 0.01852061278643439 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 610131.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.09193486158225 #Duplication Level Percentage of deduplicated Percentage of total 1 94.3156693302818 84.02765468399997 2 4.478562082864085 7.980075227201583 3 0.6337574203722816 1.6938802444155507 4 0.2053956786053863 0.7319639367664623 5 0.0963563290871996 0.4292285897268985 6 0.06225159527815329 0.3327669042930489 7 0.04230224483432048 0.26381521888945886 8 0.025451232086367077 0.18139996089485022 9 0.01910651508215906 0.1532012757418398 >10 0.10309598736173586 1.6549762822574876 >50 0.009578436242640655 0.6039160112056978 >100 0.007367954699618127 1.2906963247836005 >500 0.0011051932043738195 0.6564253398235712 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 783 0.12833309567945245 No Hit GTATCAACGCAGAGTACTTTTTTTT 724 0.11866304121573891 No Hit GTCCTACAGTGTGCATTTCTCATTT 691 0.11325436668518728 No Hit CTGTAGGACGTGGAATATGGCAAGA 674 0.1104680798058122 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.638992281985344E-4 0.0 5 0.0 0.0 0.0 1.638992281985344E-4 0.0 6 0.0 0.0 0.0 1.638992281985344E-4 0.0 7 0.0 0.0 0.0 1.638992281985344E-4 0.0 8 0.0 0.0 0.0 1.638992281985344E-4 0.0 9 0.0 0.0 0.0 1.638992281985344E-4 0.0 10 0.0 0.0 0.0 1.638992281985344E-4 0.0 11 0.0 0.0 0.0 1.638992281985344E-4 0.0 12 0.0 0.0 0.0 1.638992281985344E-4 0.0 13 0.0 0.0 0.0 1.638992281985344E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCATA 45 6.510808E-4 14.849396 1 ATAAGGC 45 6.568073E-4 14.832262 3 ACGTTTT 40 0.0053414335 14.222832 19 TAGGACC 190 0.0 14.033113 4 ACGTGAA 130 1.8189894E-12 13.898009 8 GTGTAAG 120 4.7293724E-11 13.523557 1 GTAGGAC 440 0.0 13.435717 3 GACGTGA 125 9.822543E-11 12.942037 7 TGGACTA 60 4.027346E-4 12.6882715 5 GGACGTG 315 0.0 12.688271 6 TGTAGGA 485 0.0 12.583339 2 GTCCTAA 185 0.0 12.384051 1 GAGTTAG 55 0.0029557373 12.149505 1 GTCTTAT 55 0.0029557373 12.149505 1 CGTGAAA 150 1.4551915E-11 12.012362 9 GTCCTAG 120 9.271389E-9 11.932549 1 GACGTGG 200 0.0 11.895255 7 TAGGACG 370 0.0 11.838745 4 CTGTAGG 485 0.0 11.809533 1 TTAGGAC 195 0.0 11.735416 3 >>END_MODULE