FastQCFastQC Report
Thu 26 May 2016
SRR1926965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926965_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610131
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12960.2124133997453006No Hit
GGTATCAACGCAGAGTACTTTTTTT8450.13849484782776159No Hit
GTCCTACAGTGGACATTTCTAAATT7890.12931649104864365No Hit
GTACATGGGAAGCAGTGGTATCAAC7510.12308832037709935No Hit
CTGTAGGACGTGGAATATGGCAAGA7510.12308832037709935No Hit
GTCCTACAGTGTGCATTTCTCATTT7480.12259662269250374No Hit
TATCAACGCAGAGTACTTTTTTTTT6510.1066983975572459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACGT2400.015.0794455
GGACGTG2150.014.6180186
ATCTAGG602.4723508E-514.3046211
TAGGACG2400.014.285794
ACCGTGG400.005318830714.2319278
GACGTGG1101.891749E-1013.8006567
TGTAGGA4000.013.349882
TGTGCAA500.001513052713.282042510
GTAGGAC3950.013.261073
TAGGACC1800.013.2275844
CTGTAGG4150.013.0982071
GACGTGA1251.03682396E-1012.9036137
CCTCTAA751.4181722E-512.71521951
GTATAGG603.9558817E-412.7152191
ATAGGAC1051.9161234E-812.6984813
CTCTTAG701.0505873E-412.2611051
CTAGCAC701.063593E-412.2449643
TTAAGCT550.003007708112.1212764
GTCCTAC3750.011.9523071
GTGCTAG802.7498156E-511.9205191
Produced by FastQC (version 0.11.5)