##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926965_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 610131 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.034487675597536 31.0 30.0 33.0 26.0 34.0 2 30.33909275221223 31.0 30.0 34.0 26.0 34.0 3 30.399971481534294 31.0 30.0 34.0 26.0 34.0 4 34.0135561051643 37.0 35.0 37.0 30.0 37.0 5 33.99169686509946 37.0 35.0 37.0 30.0 37.0 6 33.911119087540214 37.0 35.0 37.0 30.0 37.0 7 33.91464947691561 37.0 35.0 37.0 30.0 37.0 8 33.950918737123665 37.0 35.0 37.0 30.0 37.0 9 35.51826738847887 39.0 35.0 39.0 30.0 39.0 10 35.438748399933786 39.0 35.0 39.0 30.0 39.0 11 35.46006677254557 39.0 35.0 39.0 30.0 39.0 12 35.357539610345974 39.0 35.0 39.0 30.0 39.0 13 35.37300678051107 39.0 35.0 39.0 30.0 39.0 14 36.49853556039604 40.0 37.0 41.0 30.0 41.0 15 36.47184784906848 40.0 37.0 41.0 30.0 41.0 16 36.44613206016413 40.0 37.0 41.0 30.0 41.0 17 36.43432639875699 40.0 37.0 41.0 30.0 41.0 18 36.40026486115277 40.0 37.0 41.0 30.0 41.0 19 36.479656008299855 40.0 37.0 41.0 30.0 41.0 20 36.445279784177494 40.0 37.0 41.0 30.0 41.0 21 36.399909199827576 40.0 37.0 41.0 30.0 41.0 22 36.314742243878776 40.0 37.0 41.0 30.0 41.0 23 36.253866792541274 40.0 37.0 41.0 30.0 41.0 24 36.17437238888042 40.0 37.0 41.0 29.0 41.0 25 36.06499423894213 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3411840476668111 1101 2 -0.23722103597836508 1101 3 -0.018133362174399537 1101 4 0.005297941244400306 1101 5 -0.23974343333235737 1101 6 -0.41300151955456244 1101 7 -0.3761825597543478 1101 8 -0.5017672217634939 1101 9 -0.3889842747591885 1101 10 -0.3461411751406587 1101 11 -0.2766792031847203 1101 12 -0.391951653684977 1101 13 -0.3471948841036152 1101 14 -0.40256463403032683 1101 15 -0.39505895871776175 1101 16 -0.43928220423405406 1101 17 -0.5044756290634851 1101 18 -0.5945880767069553 1101 19 -0.5624592728528768 1101 20 -0.5848673979036931 1101 21 -0.3754578979515628 1101 22 -0.6557405064455608 1101 23 -0.40361196014457335 1101 24 -0.23642595791572063 1101 25 -0.24314837644741516 1102 1 -0.6450733689200483 1102 2 -0.414017265108626 1102 3 -0.3122920382584482 1102 4 -0.23718259012322562 1102 5 -0.3726563127320688 1102 6 -0.5337559871835253 1102 7 -0.5133366886707122 1102 8 -0.2779281200371315 1102 9 -0.3639136688454201 1102 10 -0.0817495607248162 1102 11 -0.17818334164572747 1102 12 -0.03271561701395598 1102 13 -0.1972974278743891 1102 14 0.06699528432622515 1102 15 -0.38238415707510853 1102 16 -0.23388384982907695 1102 17 -0.25502016340708167 1102 18 -0.28808499060445314 1102 19 -0.35201693965169767 1102 20 -0.23710451630137186 1102 21 -0.1124686124295664 1102 22 -0.20482201773027242 1102 23 -0.20898926737941537 1102 24 -0.17774595811150817 1102 25 -0.24702252415939085 1103 1 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0.5359114636035045 2214 6 0.4689078310727126 2214 7 0.4943242512609487 2214 8 0.476612081292366 2214 9 0.4585812585179241 2214 10 0.28182223213212865 2214 11 0.26768664563710587 2214 12 0.3535303692007261 2214 13 0.4972762189300113 2214 14 0.4290972711362784 2214 15 0.5747594505271749 2214 16 0.5328755506914291 2214 17 0.4479071911828072 2214 18 0.44024932405880435 2214 19 0.20145204339136313 2214 20 0.5133589705644965 2214 21 0.5023437054863038 2214 22 0.42324591510735843 2214 23 0.4700764481633115 2214 24 0.21756182562234727 2214 25 0.20821132055991853 2215 1 0.40181120233104295 2215 2 0.1955037301720708 2215 3 0.36717727765862307 2215 4 0.24107799810699504 2215 5 0.377052505544647 2215 6 0.3365861836948554 2215 7 0.09584782995197116 2215 8 0.3571456891374609 2215 9 0.12782755472174756 2215 10 0.25825794529293944 2215 11 0.1593911793064109 2215 12 0.22483569567638995 2215 13 0.4073489696684689 2215 14 0.47042864037892684 2215 15 0.3926876677499678 2215 16 0.3231554144086317 2215 17 0.2929277603593903 2215 18 0.3473317659331201 2215 19 0.11703583541797258 2215 20 0.09966116653055934 2215 21 0.09380452034066877 2215 22 0.09307414039650297 2215 23 0.23037159952249198 2215 24 0.0827905064678518 2215 25 -0.033924954556177056 2216 1 0.021461007778512453 2216 2 0.03986170682576784 2216 3 -1.5598655238977983 2216 4 -1.1344083832159626 2216 5 -0.307772397179086 2216 6 -0.06516478906779355 2216 7 -0.2426735708262342 2216 8 -0.06308777389755704 2216 9 0.03152405277622705 2216 10 -0.23785100412339943 2216 11 -0.15383839267802557 2216 12 -0.4823627479033732 2216 13 -0.2920673727439862 2216 14 -0.33112777985454045 2216 15 -0.29602828555742633 2216 16 -0.14668894356802298 2216 17 -0.20026290111920986 2216 18 -0.18185111344431704 2216 19 -0.1952209739205486 2216 20 -0.34489136265232645 2216 21 -0.15619547965933123 2216 22 -0.2668480385918244 2216 23 -0.47293579347361714 2216 24 -0.4541744740768934 2216 25 -0.2975436315989768 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13177.0 3 11.0 4 216.0 5 1445.0 6 1905.0 7 1485.0 8 1142.0 9 966.0 10 873.0 11 803.0 12 691.0 13 718.0 14 635.0 15 653.0 16 780.0 17 801.0 18 863.0 19 1002.0 20 1288.0 21 1692.0 22 2164.0 23 2698.0 24 3269.0 25 3854.0 26 4765.0 27 5048.0 28 5861.0 29 7338.0 30 9380.0 31 12260.0 32 16033.0 33 20829.0 34 28795.0 35 40669.0 36 59840.0 37 104429.0 38 251753.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.19190760551177 19.1879708625225 10.23241388477273 28.387707647193 2 14.420055494569507 20.574669075478848 40.04535392966203 24.959921500289614 3 18.757366284016044 24.032404273141402 29.157616758162945 28.052612684679605 4 11.800252732609883 17.03650527509666 37.98217104195656 33.181070950336895 5 12.666489645925735 38.57101645965074 35.67787470209704 13.08461919232648 6 32.069822646792225 37.937399137663 17.494155947772843 12.498622267771923 7 28.65585175616617 31.674595342463057 21.204317221346116 18.465235680024655 8 25.045157864024837 38.35451627813966 19.498362515202874 17.10196334263263 9 25.92368579357235 15.809488217177007 19.74698524626977 38.51984074298087 10 13.950740111225663 30.2607230487567 34.420294012073214 21.36824282794442 11 34.56256320571175 23.18463361585023 23.62652042005471 18.62628275838331 12 22.686513835598703 25.526405071714343 31.563325132881737 20.22375595980522 13 28.734298376734063 22.00751317756274 25.671662339705765 23.58652610599743 14 20.21615030214823 22.60022847552411 27.6670092160536 29.516612006274062 15 22.72100703977184 31.181845747863076 23.163389908294473 22.93375730407061 16 21.664049720877326 29.383930532381836 27.40171702238554 21.550302724355298 17 21.247275170865226 29.260485470309604 27.7399898382312 21.75224952059397 18 21.079977184965678 28.535183011977892 29.424838229605786 20.960001573450644 19 22.69569222246443 27.799367019105798 27.82395198392471 21.680988774505064 20 22.70186813871233 27.870800457610397 28.47674086991867 20.9505905337586 21 22.364691746184224 27.33491466855072 27.68243685177687 22.617956733488185 22 22.127379202171337 28.489291643925647 27.71519558914397 21.668133564759042 23 21.598675692065626 28.231196630226346 28.017963384852408 22.152164292855623 24 22.06791995148575 27.64165014013407 28.6983101961877 21.592119712192485 25 21.89611368814437 27.844744218066186 28.30959366650822 21.949548427281222 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 186.0 1 186.0 2 192.0 3 198.0 4 198.0 5 198.0 6 341.0 7 484.0 8 484.0 9 484.0 10 818.5 11 1153.0 12 1153.0 13 1153.0 14 1920.5 15 2688.0 16 2688.0 17 2688.0 18 4835.5 19 6983.0 20 6983.0 21 6983.0 22 10925.5 23 14868.0 24 14868.0 25 14868.0 26 21197.5 27 27527.0 28 27527.0 29 27527.0 30 35959.0 31 44391.0 32 44391.0 33 44391.0 34 51682.5 35 58974.0 36 58974.0 37 58974.0 38 65119.0 39 71264.0 40 71264.0 41 71264.0 42 74968.0 43 78672.0 44 78672.0 45 78672.0 46 81081.5 47 83491.0 48 83491.0 49 83491.0 50 79761.0 51 76031.0 52 76031.0 53 76031.0 54 66588.0 55 57145.0 56 57145.0 57 57145.0 58 48430.5 59 39716.0 60 39716.0 61 39716.0 62 31936.0 63 24156.0 64 24156.0 65 24156.0 66 18479.0 67 12802.0 68 12802.0 69 12802.0 70 9299.5 71 5797.0 72 5797.0 73 5797.0 74 4071.0 75 2345.0 76 2345.0 77 2345.0 78 1615.0 79 885.0 80 885.0 81 885.0 82 576.0 83 267.0 84 267.0 85 267.0 86 178.0 87 89.0 88 89.0 89 89.0 90 52.5 91 16.0 92 16.0 93 16.0 94 9.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1219410257797096 2 0.11505725819537116 3 0.014423132081471028 4 0.0 5 0.005408674530551635 6 0.36844546499030534 7 0.01786501587364025 8 0.008031062181728187 9 0.007867162953529652 10 0.0032779845639706886 11 0.0032779845639706886 12 3.277984563970688E-4 13 4.916976845956032E-4 14 0.0 15 0.004261379933161895 16 8.194961409926721E-4 17 1.638992281985344E-4 18 0.0011472945973897408 19 3.277984563970688E-4 20 8.194961409926721E-4 21 0.016062124363456373 22 0.0 23 1.638992281985344E-4 24 1.638992281985344E-4 25 0.006719868356139911 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 610131.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.72872419720592 #Duplication Level Percentage of deduplicated Percentage of total 1 92.82048268518884 80.50202042655269 2 5.685197967837569 9.861399331182001 3 0.8081918622930142 2.1028034736971115 4 0.24922051887267366 0.8645831058237066 5 0.12907843608820457 0.5597404041650283 6 0.07259901768441855 0.3777852109044007 7 0.040368233424789776 0.24507597681189155 8 0.030090082648656667 0.20877395832851806 9 0.023895545193170595 0.18651871337403259 >10 0.12062325446814141 1.8904412083613622 >50 0.009653063833672638 0.5799635035403715 >100 0.008328046938931502 1.1999711991903108 >500 0.0020820117339661603 1.208178936250602 >1k 1.8927379399692366E-4 0.21274455181804078 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1296 0.2124133997453006 No Hit GGTATCAACGCAGAGTACTTTTTTT 845 0.13849484782776159 No Hit GTCCTACAGTGGACATTTCTAAATT 789 0.12931649104864365 No Hit GTACATGGGAAGCAGTGGTATCAAC 751 0.12308832037709935 No Hit CTGTAGGACGTGGAATATGGCAAGA 751 0.12308832037709935 No Hit GTCCTACAGTGTGCATTTCTCATTT 748 0.12259662269250374 No Hit TATCAACGCAGAGTACTTTTTTTTT 651 0.1066983975572459 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGACGT 240 0.0 15.079445 5 GGACGTG 215 0.0 14.618018 6 ATCTAGG 60 2.4723508E-5 14.304621 1 TAGGACG 240 0.0 14.28579 4 ACCGTGG 40 0.0053188307 14.231927 8 GACGTGG 110 1.891749E-10 13.800656 7 TGTAGGA 400 0.0 13.34988 2 TGTGCAA 50 0.0015130527 13.2820425 10 GTAGGAC 395 0.0 13.26107 3 TAGGACC 180 0.0 13.227584 4 CTGTAGG 415 0.0 13.098207 1 GACGTGA 125 1.03682396E-10 12.903613 7 CCTCTAA 75 1.4181722E-5 12.7152195 1 GTATAGG 60 3.9558817E-4 12.715219 1 ATAGGAC 105 1.9161234E-8 12.698481 3 CTCTTAG 70 1.0505873E-4 12.261105 1 CTAGCAC 70 1.063593E-4 12.244964 3 TTAAGCT 55 0.0030077081 12.121276 4 GTCCTAC 375 0.0 11.952307 1 GTGCTAG 80 2.7498156E-5 11.920519 1 >>END_MODULE