FastQCFastQC Report
Thu 26 May 2016
SRR1926964_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926964_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579021
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11170.19291182875923327No Hit
GTCCTACAGTGGACATTTCTAAATT8870.15318960797622194No Hit
GTCCTACAGTGTGCATTTCTCATTT8140.14058212051030963No Hit
CTGTAGGACGTGGAATATGGCAAGA7100.12262076850407842No Hit
GGTATCAACGCAGAGTACTTTTTTT6550.11312197657770617No Hit
GTACATGGGAAGCAGTGGTATCAAC6040.10431400588234278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAAA250.006090497618.96588311
CTAGGAC807.352355E-915.4887093
TAGGACC2300.014.9007814
TCCAACG456.8459084E-414.75124318
GTTGTAG759.18928E-713.9929161
GTCCTAT551.8872115E-413.8772711
TTAGCCT551.9222888E-413.8471894
GTACAAT500.001450154813.35687351
GTAAGAC801.9204235E-613.1058313
GGCGAGG1450.013.07991919
TAGGACT1403.6379788E-1212.9199234
TGGCGAG3800.012.72710718
AATCCCG854.01103E-612.27204319
TCCTAAA2500.012.2003372
TATGGCG4450.012.14668916
TTTAGTA550.002989339912.1310162
ATAGGGC550.00303829312.1047468
CTAAATT3150.012.0418319
ATGGCGA4650.012.0321217
TAAGACA951.0100885E-612.0251914
Produced by FastQC (version 0.11.5)