##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926964_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 579021 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.197253640196124 31.0 28.0 31.0 16.0 34.0 2 28.210676296714627 31.0 28.0 31.0 16.0 34.0 3 27.80614520026044 31.0 28.0 31.0 16.0 34.0 4 31.62192908374653 35.0 32.0 37.0 19.0 37.0 5 31.478949813564622 35.0 32.0 37.0 18.0 37.0 6 31.06400804115913 35.0 32.0 37.0 16.0 37.0 7 30.920600461813994 35.0 32.0 37.0 16.0 37.0 8 31.052714841085212 35.0 32.0 37.0 11.0 37.0 9 33.191647625906484 37.0 33.0 39.0 17.0 39.0 10 33.37447864585222 37.0 34.0 39.0 17.0 39.0 11 33.519406031905575 38.0 34.0 39.0 19.0 39.0 12 33.41568785933498 38.0 34.0 39.0 17.0 39.0 13 33.50580030776086 38.0 34.0 39.0 18.0 39.0 14 34.53504277046947 39.0 34.0 41.0 18.0 41.0 15 34.1209058047981 39.0 33.0 41.0 16.0 41.0 16 34.20433801192012 39.0 34.0 41.0 17.0 41.0 17 34.434345213731454 39.0 34.0 41.0 17.0 41.0 18 34.476632108334584 39.0 34.0 41.0 17.0 41.0 19 33.89332165845453 39.0 33.0 41.0 10.0 41.0 20 34.16836522336841 39.0 34.0 41.0 16.0 41.0 21 34.16297681776654 39.0 34.0 41.0 11.0 41.0 22 34.19282720315844 39.0 34.0 41.0 10.0 41.0 23 34.188241877237616 39.0 34.0 41.0 2.0 41.0 24 32.902245341706085 39.0 32.0 41.0 2.0 41.0 25 33.47813464451203 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.1736315270950506 1101 2 0.0840771788348782 1101 3 -0.6415051209332674 1101 4 -0.1495014566674442 1101 5 -0.15663766322305506 1101 6 -1.6787716040620921 1101 7 -0.11815838838415615 1101 8 0.5463265301778577 1101 9 0.454464118090975 1101 10 -9.158144907411465 1101 11 -3.4346067207883557 1101 12 -0.894767444725673 1101 13 -0.3746805310622605 1101 14 0.005624629844675155 1101 15 -1.0253746305941718 1101 16 0.2393784423180776 1101 17 0.385487109701387 1101 18 0.6498943674735784 1101 19 -0.0025787225011626447 1101 20 0.5464071067908236 1101 21 0.5108864705917853 1101 22 0.7692967184686523 1101 23 0.7074512214906648 1101 24 0.7771205464707052 1101 25 0.8709724320567389 1102 1 -0.09031630749299069 1102 2 -0.44673094117062107 1102 3 -0.650781664879414 1102 4 -0.33291369440730634 1102 5 -0.6180384989698169 1102 6 -0.9019601045788157 1102 7 -0.32057712501499935 1102 8 0.09688594131543127 1102 9 0.3903765838979325 1102 10 0.35237953797701493 1102 11 -0.07050815767912866 1102 12 0.1361107974012512 1102 13 -0.08285268563592751 1102 14 -0.11130911360095297 1102 15 0.2228917190932691 1102 16 0.2306873840459147 1102 17 0.11657217804662423 1102 18 -0.3585148407183212 1102 19 -0.05396938713632693 1102 20 -0.06906496486173097 1102 21 -0.268095410668991 1102 22 -0.2508507966892992 1102 23 -0.63477604770884 1102 24 -4.5709112004687675 1102 25 -2.2877268638627015 1103 1 0.09346322159908382 1103 2 -0.13775376341963863 1103 3 0.2985257130303829 1103 4 0.22058877194201187 1103 5 -0.23794674270937577 1103 6 0.15858957076978797 1103 7 -0.04430567222608417 1103 8 0.373623267812242 1103 9 0.11401334703522537 1103 10 0.25267839248396484 1103 11 -0.10685326023391895 1103 12 -0.0409559782937734 1103 13 -0.02581861844720379 1103 14 -0.1146898269857104 1103 15 0.05141681856078861 1103 16 -0.035746484833580894 1103 17 -0.11761488623977101 1103 18 -0.3051938565149115 1103 19 -1.7865425814585123 1103 20 -0.3889366340385507 1103 21 -0.21575762858941516 1103 22 0.01909728968229274 1103 23 0.13797353891516195 1103 24 1.5037398462173428 1103 25 0.7071721667027902 1104 1 0.09632779097210786 1104 2 0.009273970945159249 1104 3 -1.2327818034402043 1104 4 -0.4807729981529292 1104 5 -0.44239232345133317 1104 6 -1.8730416530363776 1104 7 -0.47341239118076217 1104 8 0.051359213346934496 1104 9 0.30459930542199487 1104 10 0.37676895356293016 1104 11 0.2083318440053361 1104 12 0.338042769488041 1104 13 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1108 22 0.3003206224079733 1108 23 0.401498387861146 1108 24 -0.8851525652836365 1108 25 -0.18711380423037838 1109 1 0.9400112122185433 1109 2 0.7461197581755243 1109 3 -0.4546713394701598 1109 4 0.8228547370215153 1109 5 1.1143208500315147 1109 6 1.2373905181989286 1109 7 1.3619677254063838 1109 8 1.7165571990832582 1109 9 0.8701245644868791 1109 10 1.0578447199459973 1109 11 0.7842731205112372 1109 12 0.35098488421051144 1109 13 0.6244334086422896 1109 14 0.6932239929011885 1109 15 -0.6343976566876961 1109 16 0.4946849235622821 1109 17 0.33680733954160047 1109 18 0.6003132381355485 1109 19 -0.935370730364788 1109 20 0.40094660404403726 1109 21 0.32915996616977594 1109 22 0.630066685741788 1109 23 0.48873456827426054 1109 24 -0.5116343921998947 1109 25 0.0911322798838512 1110 1 0.6880726976526077 1110 2 0.5970111662626394 1110 3 1.0219448558251223 1110 4 0.699000387935186 1110 5 0.6681206048352912 1110 6 0.8602954278115647 1110 7 1.0864317973822466 1110 8 1.4453273330698089 1110 9 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2216 12 -2.213004433207562 2216 13 -1.8439463510782623 2216 14 -1.9388021809974987 2216 15 -2.9999727330600656 2216 16 -1.9591933547758273 2216 17 -2.1773846076252354 2216 18 -1.8454901857285861 2216 19 -1.4302906615594324 2216 20 -2.3952855050468287 2216 21 -4.596751811522864 2216 22 -2.985365982420678 2216 23 -3.278896804001583 2216 24 -2.9438306694751404 2216 25 -2.5518809542487304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33210.0 3 2.0 4 229.0 5 1499.0 6 1869.0 7 1528.0 8 1260.0 9 1098.0 10 1075.0 11 1033.0 12 1032.0 13 995.0 14 971.0 15 1050.0 16 1151.0 17 1225.0 18 1453.0 19 1743.0 20 2328.0 21 3061.0 22 3837.0 23 4491.0 24 5298.0 25 6002.0 26 6932.0 27 7561.0 28 8508.0 29 9934.0 30 11919.0 31 14467.0 32 18777.0 33 24582.0 34 33237.0 35 46466.0 36 68778.0 37 109356.0 38 141064.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.42887607600261 18.87446621788869 12.533645366156787 25.163012339951912 2 13.94888751309978 20.51486025172329 42.688507126626014 22.847745108550914 3 19.762486986417453 24.41434555082474 29.017954420467557 26.805213042290255 4 11.1220567699476 17.184972416939733 39.43876422359561 32.254206589517054 5 12.23965216129517 38.505654797379115 36.147344309530844 13.107348731794868 6 30.71443131212277 38.69084841093534 18.472984691497928 12.121735585443966 7 27.474868909510754 31.60411105317588 22.085217912495477 18.835802124817892 8 23.967629288842083 38.17259991131312 21.495482512055872 16.364288287788924 9 25.882210687233876 16.23830786903086 20.386930723854107 37.49255071988116 10 13.61326012667194 30.516155337256272 35.415450106942075 20.45513442912971 11 34.26625970970634 23.511091995419143 24.006142355479323 18.216505939395194 12 22.27187380705206 25.86008847463307 32.53869685605858 19.32934086225629 13 28.211187054895436 22.77536243149082 25.981065297260326 23.032385216353422 14 19.718582340402747 23.17498264026836 28.552526575071767 28.553908444257125 15 22.67674944424389 31.91413287364343 23.49911303569794 21.910004646414745 16 20.940697274710622 29.854502678304613 28.662264147684187 20.542535899300585 17 20.681477028738044 29.699664374463442 28.418310679041454 21.200547917757063 18 20.22441051647928 29.284086924529184 30.292483318622516 20.19901924036902 19 22.60827354844951 27.979498377041224 28.22013505366388 21.19209302084539 20 22.14096077743826 28.636943863388648 29.2491108550819 19.97298450409119 21 22.078529998030877 27.90540610566244 28.006971385536968 22.00909251076972 22 21.311112953596993 29.37947378603211 28.621521156344137 20.687892104026755 23 21.393012481905902 28.69370100774203 28.409032021806198 21.504254488545875 24 21.748473039825335 28.218362214299532 29.24405107370865 20.789113672166486 25 21.470174699190586 28.39423219757556 28.940404672002874 21.195188431230978 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 190.0 1 190.0 2 275.5 3 361.0 4 361.0 5 361.0 6 530.0 7 699.0 8 699.0 9 699.0 10 1101.5 11 1504.0 12 1504.0 13 1504.0 14 2442.0 15 3380.0 16 3380.0 17 3380.0 18 5650.5 19 7921.0 20 7921.0 21 7921.0 22 12409.0 23 16897.0 24 16897.0 25 16897.0 26 23327.0 27 29757.0 28 29757.0 29 29757.0 30 38785.5 31 47814.0 32 47814.0 33 47814.0 34 54465.5 35 61117.0 36 61117.0 37 61117.0 38 65869.5 39 70622.0 40 70622.0 41 70622.0 42 73652.0 43 76682.0 44 76682.0 45 76682.0 46 77224.5 47 77767.0 48 77767.0 49 77767.0 50 73343.0 51 68919.0 52 68919.0 53 68919.0 54 59076.0 55 49233.0 56 49233.0 57 49233.0 58 40652.5 59 32072.0 60 32072.0 61 32072.0 62 25337.5 63 18603.0 64 18603.0 65 18603.0 66 13973.5 67 9344.0 68 9344.0 69 9344.0 70 6620.0 71 3896.0 72 3896.0 73 3896.0 74 2686.5 75 1477.0 76 1477.0 77 1477.0 78 1004.5 79 532.0 80 532.0 81 532.0 82 346.0 83 160.0 84 160.0 85 160.0 86 106.0 87 52.0 88 52.0 89 52.0 90 34.5 91 17.0 92 17.0 93 17.0 94 10.0 95 3.0 96 3.0 97 3.0 98 2.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.225207721308899 2 0.13246497104595517 3 0.13453743473898183 4 0.06994564963965037 5 0.022797100623293456 6 0.02193357408453234 7 0.30188887795088604 8 0.2951533709485493 9 0.018306762621735653 10 0.035059177473701304 11 0.015543477697700083 12 0.01692512015971787 13 0.014507245851186745 14 0.0164070042364612 15 0.013816424620177853 16 0.018997583852744547 17 0.017615941390726763 18 0.014161835235682298 19 0.024178743085311243 20 0.01623429892870898 21 0.014334540543434521 22 0.01606159362095675 23 0.017788646698478985 24 0.01606159362095675 25 0.014334540543434521 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 579021.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.70966719053057 #Duplication Level Percentage of deduplicated Percentage of total 1 95.04194447189282 84.31139263242497 2 3.8463174802758107 6.824110871687745 3 0.5292514307964216 1.4084915485818803 4 0.19602230901996712 0.6955629518032249 5 0.10333904090756259 0.45835859633492504 6 0.054946076652599894 0.29245489039665107 7 0.03897443260812324 0.24201862619244624 8 0.028593048792274575 0.20291838738602236 9 0.017625756461615075 0.14072174907220952 >10 0.11997871413574179 2.0162569838043383 >50 0.012283554124336689 0.7390723351631975 >100 0.008968899623950614 1.5573555257393856 >500 0.0015598086300888344 0.9180856430662095 >1k 1.949760787611043E-4 0.1931992583468267 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1117 0.19291182875923327 No Hit GTCCTACAGTGGACATTTCTAAATT 887 0.15318960797622194 No Hit GTCCTACAGTGTGCATTTCTCATTT 814 0.14058212051030963 No Hit CTGTAGGACGTGGAATATGGCAAGA 710 0.12262076850407842 No Hit GGTATCAACGCAGAGTACTTTTTTT 655 0.11312197657770617 No Hit GTACATGGGAAGCAGTGGTATCAAC 604 0.10431400588234278 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGAAA 25 0.0060904976 18.965883 11 CTAGGAC 80 7.352355E-9 15.488709 3 TAGGACC 230 0.0 14.900781 4 TCCAACG 45 6.8459084E-4 14.751243 18 GTTGTAG 75 9.18928E-7 13.992916 1 GTCCTAT 55 1.8872115E-4 13.877271 1 TTAGCCT 55 1.9222888E-4 13.847189 4 GTACAAT 50 0.0014501548 13.3568735 1 GTAAGAC 80 1.9204235E-6 13.105831 3 GGCGAGG 145 0.0 13.079919 19 TAGGACT 140 3.6379788E-12 12.919923 4 TGGCGAG 380 0.0 12.727107 18 AATCCCG 85 4.01103E-6 12.272043 19 TCCTAAA 250 0.0 12.200337 2 TATGGCG 445 0.0 12.146689 16 TTTAGTA 55 0.0029893399 12.131016 2 ATAGGGC 55 0.003038293 12.104746 8 CTAAATT 315 0.0 12.04183 19 ATGGCGA 465 0.0 12.03212 17 TAAGACA 95 1.0100885E-6 12.025191 4 >>END_MODULE