FastQCFastQC Report
Thu 26 May 2016
SRR1926964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926964_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579021
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18380.31743235564858613No Hit
GGTATCAACGCAGAGTACTTTTTTT11790.2036195578398711No Hit
TATCAACGCAGAGTACTTTTTTTTT9680.167178737904152No Hit
GTCCTACAGTGGACATTTCTAAATT9410.162515694594842No Hit
CTGTAGGACGTGGAATATGGCAAGA8630.14904468059016857No Hit
GTCCTACAGTGTGCATTTCTCATTT7750.13384661350797294No Hit
GTACATGGGAAGCAGTGGTATCAAC6710.11588526150174173No Hit
CACACACACACACACACACACACAC6380.11018598634591836No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6350.1096678704226617No Hit
CTGTAGGACCTGGAATATGGCGAGA6060.10465941649784724No Hit
GTCCTAAAGTGTGTATTTCTCATTT5850.10103260503505054No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGG402.7187212E-416.6557733
GCCGGTT456.804926E-414.7629311
CCAACGT400.005309094714.235682519
GTAGTGC400.00531518314.2332248
TAGGACC3100.013.2018124
GGCGAGG2000.012.81211519
TCTATAC751.44699825E-512.6901143
CCGGTTT604.119922E-412.6539412
TGTAGGA5900.012.5956632
TCCAACG853.975678E-612.28176618
ATAGGAC1405.0931703E-1112.2368963
TAGACAG701.06997875E-412.2368965
GTAGGAC5650.012.1286043
GTATAAG550.002994923412.1279981
TGGCGAG5450.012.01543818
CTGTAGG6350.011.7050881
CGGTTTC658.077911E-411.6805613
GGACATG2150.011.508646
GTCCTAC4400.011.2617131
TAGGACG4150.011.2376314
Produced by FastQC (version 0.11.5)