FastQCFastQC Report
Thu 26 May 2016
SRR1926963_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926963_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651592
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13070.20058564254932534No Hit
CTGTAGGACGTGGAATATGGCAAGA10070.15454456162752153No Hit
GTATCAACGCAGAGTACTTTTTTTT9910.152089037311692No Hit
GTCCTACAGTGTGCATTTCTCATTT8950.13735589141671475No Hit
GTCCTAAAGTGTGTATTTCTCATTT8700.1335191346732311No Hit
CTTTAGGACGTGAAATATGGCGAGG6670.10236466991614385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACAAT551.9297432E-413.8413726
TAGGACC2650.013.6518114
GGTTACA500.001453530513.3531721
CTAGCAC655.2925094E-513.1931953
AATCCCG701.1057163E-412.19439919
GGACCTG3450.012.1362746
TAGGGTC802.811678E-511.8949285
GTGTAGA1302.4429028E-911.7390541
GTAGGAC6350.011.5541453
GTATTAG751.9949334E-411.4455781
GTCCTAT1001.8335159E-611.4455771
CTTCTAT1251.7185812E-811.4455771
TAGTACT1001.8748251E-611.4244114
GACCTGG3500.011.4182527
TGTAGGA6950.011.3801392
CTGTAGG6700.011.2462761
GATATAC1451.1332304E-911.182461
ATAGGAC600.00574034111.11648753
TAATGCT600.005776700611.1070654
GACGTGG2750.011.0722457
Produced by FastQC (version 0.11.5)