FastQCFastQC Report
Thu 26 May 2016
SRR1926963_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926963_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651592
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16450.2524585937212243No Hit
CTGTAGGACGTGGAATATGGCAAGA13180.20227381551645812No Hit
GTCCTACAGTGGACATTTCTAAATT12300.1887684317793957No Hit
GTCCTACAGTGTGCATTTCTCATTT10100.15500497243673955No Hit
GGTATCAACGCAGAGTACTTTTTTT10020.1537772102788248No Hit
GTCCTAAAGTGTGTATTTCTCATTT9360.14364817247602796No Hit
TATCAACGCAGAGTACTTTTTTTTT8710.13367260494297045No Hit
CTTTAGGACGTGAAATATGGCGAGG8460.1298358481994868No Hit
CTGTAGGACCTGGAATATGGCGAGA7540.11571658338346696No Hit
GTACATGGGAAGCAGTGGTATCAAC7240.11111247529128658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG653.4007553E-614.60044718
GTCTTAC400.005168922214.2935841
GACTGTC602.5942127E-514.2321597
TAGGACC3150.012.6917134
TGTAGGA7450.012.4060022
GACGTGG3500.012.1989937
AGGACGT6100.012.1715625
TAAGGCC550.00302012412.1148184
GTAGGAC7400.012.0951553
TAGGACT802.8031005E-511.8984814
TAGGACG6500.011.8618714
GGACGTG6100.011.859476
GTGTAGG657.809699E-411.7280681
TCTTAAG657.814753E-411.7271642
ATGGCAA5400.011.59924317
CGTGGAA3600.011.5983529
TGGCGAG6600.011.50338218
CTGTAGG7650.011.4597811
ACGTGGA3650.011.4377168
CCACACT1001.8785267E-611.4225424
Produced by FastQC (version 0.11.5)