##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926963_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 651592 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.054348426622795 31.0 30.0 33.0 26.0 34.0 2 30.338804036881974 31.0 30.0 34.0 26.0 34.0 3 30.40114979926089 31.0 30.0 34.0 26.0 34.0 4 34.00711334700242 37.0 35.0 37.0 30.0 37.0 5 34.007202359758864 37.0 35.0 37.0 30.0 37.0 6 33.972459760095276 37.0 35.0 37.0 30.0 37.0 7 33.96735073481565 37.0 35.0 37.0 30.0 37.0 8 33.99822741838451 37.0 35.0 37.0 30.0 37.0 9 35.55513112499847 39.0 35.0 39.0 30.0 39.0 10 35.441139547446866 39.0 35.0 39.0 30.0 39.0 11 35.49228965364829 39.0 35.0 39.0 30.0 39.0 12 35.3881309162789 39.0 35.0 39.0 30.0 39.0 13 35.41612696288475 39.0 35.0 39.0 30.0 39.0 14 36.52796228314651 40.0 37.0 41.0 30.0 41.0 15 36.51539460275755 40.0 37.0 41.0 30.0 41.0 16 36.493733808886546 40.0 37.0 41.0 30.0 41.0 17 36.47416634949477 40.0 37.0 41.0 30.0 41.0 18 36.45219708038159 40.0 37.0 41.0 30.0 41.0 19 36.52179738241108 40.0 37.0 41.0 30.0 41.0 20 36.48779450944763 40.0 37.0 41.0 30.0 41.0 21 36.43588779481639 40.0 37.0 41.0 30.0 41.0 22 36.35309058429201 40.0 37.0 41.0 30.0 41.0 23 36.29017084310427 40.0 37.0 41.0 30.0 41.0 24 36.22565654581395 40.0 37.0 41.0 30.0 41.0 25 36.11336541885106 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.23705806004171492 1101 2 -0.21701248227187264 1101 3 0.1333535398002077 1101 4 -0.014260534566815863 1101 5 -0.09443888302003955 1101 6 -0.019266380446154585 1101 7 -0.08204824558316659 1101 8 -0.12570020745122434 1101 9 -0.054277173069976925 1101 10 -0.132700808529556 1101 11 -0.23944905934888538 1101 12 -0.1029335163179681 1101 13 -0.20270184263427637 1101 14 -0.04845611683231965 1101 15 -0.02664227758771176 1101 16 -0.1289192241080599 1101 17 0.1345557372925441 1101 18 0.012782723764907189 1101 19 -0.14685968129198557 1101 20 -0.08556577997475046 1101 21 -0.12628119492930523 1101 22 -0.27882548362669723 1101 23 -0.19241442784436913 1101 24 -0.13397812945201082 1101 25 -0.017194285998833436 1102 1 -0.6998029882213821 1102 2 -0.47265496699008835 1102 3 -0.5773445224314493 1102 4 -0.6406940987286589 1102 5 -0.8200911675725777 1102 6 -0.5910417747405887 1102 7 -0.5362786878751322 1102 8 -0.49837761964524674 1102 9 -0.44586664122593334 1102 10 -0.4807811779995532 1102 11 -0.41526674994483415 1102 12 -0.24483295160928265 1102 13 -0.45105888146002826 1102 14 -0.6258894265233295 1102 15 -0.5360181743266992 1102 16 -0.6253344338840705 1102 17 -0.6399906819779346 1102 18 -0.5644816606051677 1102 19 -0.547062175106177 1102 20 -0.5626184662663576 1102 21 -0.5937094693463294 1102 22 -0.5191325811973115 1102 23 -0.6222654811911994 1102 24 -0.5379370459476789 1102 25 -0.5455731984879364 1103 1 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2215 21 -0.48825959628742766 2215 22 -0.4310383695799729 2215 23 -0.4913268872458616 2215 24 -0.3652623974425637 2215 25 -0.2010870821717532 2216 1 -0.10228779043267267 2216 2 -0.10258277145326034 2216 3 -1.7945419438813524 2216 4 -1.1784773961414103 2216 5 -0.26789389109362816 2216 6 -0.16555008157645545 2216 7 -0.16629250843974575 2216 8 -0.09435999117681604 2216 9 -0.12933222028826208 2216 10 -0.08825201467526966 2216 11 -0.09916917482571819 2216 12 -0.1280056893374919 2216 13 -0.3220588911247546 2216 14 -0.17606290843578876 2216 15 -0.23537643622156423 2216 16 -0.10616637388542927 2216 17 -0.32521330904497603 2216 18 -0.4329872033281106 2216 19 -0.3200308903735518 2216 20 -0.112952382705096 2216 21 -0.031683161949906946 2216 22 -0.009304516752891345 2216 23 -0.05351928667883499 2216 24 -0.11437153357199037 2216 25 -0.17516756974889347 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13737.0 3 13.0 4 264.0 5 1400.0 6 2016.0 7 1547.0 8 1189.0 9 995.0 10 891.0 11 839.0 12 694.0 13 734.0 14 773.0 15 695.0 16 802.0 17 762.0 18 923.0 19 1104.0 20 1452.0 21 1774.0 22 2328.0 23 2817.0 24 3415.0 25 4049.0 26 4942.0 27 5539.0 28 6350.0 29 7805.0 30 10179.0 31 13002.0 32 17173.0 33 22548.0 34 30712.0 35 43297.0 36 64392.0 37 111275.0 38 269165.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.54886728209162 19.732523693041436 10.884816786104999 27.83379223876194 2 13.960630441428831 20.74667887523448 40.79264536210131 24.50004532123538 3 18.531013957086525 24.674864197379268 29.239275084919807 27.5548467606144 4 11.843147245515599 17.591529668872546 38.34178442952032 32.22353865609154 5 12.728528454785437 38.76649886426423 35.47654859107373 13.028424089876603 6 31.462236691748775 38.26012498960473 17.826881081230923 12.450757237415568 7 28.15483161900259 31.884556287405847 21.55282906784513 18.40778302574643 8 24.66759268496704 38.647501669135195 19.713906390293694 16.970999255604074 9 25.878624161588874 16.052215554157137 20.163307906005862 37.90585237824812 10 14.247542865562057 30.310694750541767 34.21080095522828 21.2309614286679 11 33.89050123624666 23.410874366337257 24.274482057924793 18.42414233949129 12 23.105102579528296 25.475757836191974 31.72875050645189 19.690389077827845 13 28.736672964092396 22.55378774043147 25.793560050890974 22.91597924458516 14 20.089104838610663 23.203170081891734 27.857156011737406 28.850569067760194 15 22.359531592921712 31.92105222769618 23.363107570944027 22.35630860843808 16 21.24652196399878 29.385241310089643 28.325370747511087 21.042865978400492 17 20.83896057655711 29.707393583714964 28.10040638927427 21.35323945045366 18 20.69187785551935 28.68664142580676 30.140471984640627 20.481008734033264 19 22.740457218627608 27.616207688246636 28.05590001104986 21.5874350820759 20 22.785528914699295 28.045286215697317 28.691859439002194 20.477325430601194 21 22.088500736738702 27.487414047151276 27.74373772102161 22.68034749508841 22 22.105857653255413 28.9894596618743 27.729161806774794 21.175520878095494 23 21.363675428795933 28.620363663151178 28.175913761986028 21.840047146066862 24 22.18278030236759 27.652929521739868 29.1179589650563 21.046331210836243 25 21.86736938067956 28.063336346572658 28.535427560243043 21.533866712504736 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 218.0 1 218.0 2 220.5 3 223.0 4 223.0 5 223.0 6 375.5 7 528.0 8 528.0 9 528.0 10 918.0 11 1308.0 12 1308.0 13 1308.0 14 2239.5 15 3171.0 16 3171.0 17 3171.0 18 5536.5 19 7902.0 20 7902.0 21 7902.0 22 12585.0 23 17268.0 24 17268.0 25 17268.0 26 24686.0 27 32104.0 28 32104.0 29 32104.0 30 41981.5 31 51859.0 32 51859.0 33 51859.0 34 59023.5 35 66188.0 36 66188.0 37 66188.0 38 71443.5 39 76699.0 40 76699.0 41 76699.0 42 80346.5 43 83994.0 44 83994.0 45 83994.0 46 86113.5 47 88233.0 48 88233.0 49 88233.0 50 83480.0 51 78727.0 52 78727.0 53 78727.0 54 68599.0 55 58471.0 56 58471.0 57 58471.0 58 48964.0 59 39457.0 60 39457.0 61 39457.0 62 31601.0 63 23745.0 64 23745.0 65 23745.0 66 18119.0 67 12493.0 68 12493.0 69 12493.0 70 9063.5 71 5634.0 72 5634.0 73 5634.0 74 3918.0 75 2202.0 76 2202.0 77 2202.0 78 1499.0 79 796.0 80 796.0 81 796.0 82 539.5 83 283.0 84 283.0 85 283.0 86 179.0 87 75.0 88 75.0 89 75.0 90 43.0 91 11.0 92 11.0 93 11.0 94 7.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1178651671598178 2 0.10482019423197339 3 0.014579675625237879 4 0.0 5 0.004911048631659075 6 0.34653586907144346 7 0.01887684317793957 8 0.008747805375142729 9 0.009515156723839457 10 0.0030694053947869218 11 0.0035298162040049604 12 0.0 13 4.604108092180383E-4 14 0.0 15 0.003376345934265614 16 4.604108092180383E-4 17 0.0 18 4.604108092180383E-4 19 0.0 20 4.604108092180383E-4 21 0.011049859421232919 22 0.0 23 0.0 24 1.534702697393461E-4 25 0.0035298162040049604 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 651592.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.02622092909739 #Duplication Level Percentage of deduplicated Percentage of total 1 92.9430236350331 79.02594061410645 2 5.5073647499404315 9.365408239311165 3 0.793530401439186 2.0241267368017066 4 0.25570155118577803 0.8696534633213947 5 0.13331901397737586 0.5667805968244891 6 0.07244025654333903 0.3695592754208662 7 0.05557931905532711 0.33079896227609246 8 0.03763642400079222 0.25600663216580344 9 0.02575501209271518 0.19708662134040983 >10 0.14671968326776963 2.334567584274464 >50 0.014826723683900452 0.8698527698966695 >100 0.011752691484260894 1.9347732571084086 >500 0.001446485105229674 0.9015105583975579 >1k 9.040531907685464E-4 0.9539346887545782 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1645 0.2524585937212243 No Hit CTGTAGGACGTGGAATATGGCAAGA 1318 0.20227381551645812 No Hit GTCCTACAGTGGACATTTCTAAATT 1230 0.1887684317793957 No Hit GTCCTACAGTGTGCATTTCTCATTT 1010 0.15500497243673955 No Hit GGTATCAACGCAGAGTACTTTTTTT 1002 0.1537772102788248 No Hit GTCCTAAAGTGTGTATTTCTCATTT 936 0.14364817247602796 No Hit TATCAACGCAGAGTACTTTTTTTTT 871 0.13367260494297045 No Hit CTTTAGGACGTGAAATATGGCGAGG 846 0.1298358481994868 No Hit CTGTAGGACCTGGAATATGGCGAGA 754 0.11571658338346696 No Hit GTACATGGGAAGCAGTGGTATCAAC 724 0.11111247529128658 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 65 3.4007553E-6 14.600447 18 GTCTTAC 40 0.0051689222 14.293584 1 GACTGTC 60 2.5942127E-5 14.232159 7 TAGGACC 315 0.0 12.691713 4 TGTAGGA 745 0.0 12.406002 2 GACGTGG 350 0.0 12.198993 7 AGGACGT 610 0.0 12.171562 5 TAAGGCC 55 0.003020124 12.114818 4 GTAGGAC 740 0.0 12.095155 3 TAGGACT 80 2.8031005E-5 11.898481 4 TAGGACG 650 0.0 11.861871 4 GGACGTG 610 0.0 11.85947 6 GTGTAGG 65 7.809699E-4 11.728068 1 TCTTAAG 65 7.814753E-4 11.727164 2 ATGGCAA 540 0.0 11.599243 17 CGTGGAA 360 0.0 11.598352 9 TGGCGAG 660 0.0 11.503382 18 CTGTAGG 765 0.0 11.459781 1 ACGTGGA 365 0.0 11.437716 8 CCACACT 100 1.8785267E-6 11.422542 4 >>END_MODULE