FastQCFastQC Report
Thu 26 May 2016
SRR1926962_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926962_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658533
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17920.2721200000607411No Hit
CTGTAGGACGTGGAATATGGCAAGA14370.21821229915585097No Hit
GTCCTACAGTGTGCATTTCTCATTT12660.19224549111434053No Hit
GTCCTAAAGTGTGTATTTCTCATTT11780.17888245539707198No Hit
CTTTAGGACGTGAAATATGGCGAGG9280.14091928574574092No Hit
CTGTAGGACCTGGAATATGGCGAGA8490.1289229241359203No Hit
GTATCAACGCAGAGTACTTTTTTTT7520.11419321431120386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG801.2312557E-714.2994691
TAGGACC2750.013.8450144
TAGACTC500.001477896413.3237935
GTCTTAG1051.891567E-812.7106391
TAAATGC751.4463647E-512.6912614
GTAGGAC8250.012.2437613
TGGGACC701.0695042E-412.2380024
CTGTAGG8950.012.0360531
TAGGACT951.0127151E-612.02334
GTCCTAC6750.012.0044921
TCCAACG802.908782E-511.85558518
TGTAGGA9450.011.5965552
TAGGAGA1251.7716957E-811.4221364
TTAGACA752.0333656E-411.4221354
TGCACTT752.1020568E-411.38136112
AGGACCT4300.011.28760055
ATAGCAC855.1474664E-511.2109913
GAACAGT855.2159274E-511.1964666
GGACCTG4600.011.1721266
TTAATCT600.00578538111.1048554
Produced by FastQC (version 0.11.5)