##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926962_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 658533 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.15546677235613 31.0 28.0 31.0 16.0 34.0 2 28.2186207828613 31.0 28.0 31.0 16.0 34.0 3 27.787854215354432 31.0 28.0 31.0 16.0 34.0 4 31.61564720370885 35.0 32.0 37.0 19.0 37.0 5 31.40040969852688 35.0 32.0 37.0 17.0 37.0 6 30.997058613615415 35.0 32.0 37.0 15.0 37.0 7 30.82677253835419 35.0 31.0 37.0 13.0 37.0 8 30.974145562940656 35.0 32.0 37.0 11.0 37.0 9 33.13229709065453 37.0 33.0 39.0 17.0 39.0 10 33.29973289113833 37.0 33.0 39.0 17.0 39.0 11 33.465425422871746 38.0 34.0 39.0 18.0 39.0 12 33.34802052440804 38.0 34.0 39.0 17.0 39.0 13 33.43888309317832 38.0 34.0 39.0 18.0 39.0 14 34.461285918852965 39.0 34.0 41.0 18.0 41.0 15 34.059019062066746 39.0 33.0 41.0 16.0 41.0 16 34.1402815044956 39.0 33.0 41.0 17.0 41.0 17 34.35654401525816 39.0 34.0 41.0 17.0 41.0 18 34.40570631995663 39.0 34.0 41.0 17.0 41.0 19 33.769352484993156 39.0 33.0 41.0 10.0 41.0 20 34.079373395107005 39.0 34.0 41.0 11.0 41.0 21 34.07779564577629 39.0 34.0 41.0 10.0 41.0 22 34.09059075247558 39.0 34.0 41.0 10.0 41.0 23 34.07117335046232 39.0 34.0 41.0 2.0 41.0 24 32.768479332091175 39.0 32.0 40.0 2.0 41.0 25 33.35556760253473 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.07348373875559133 1101 2 -0.20927831396261354 1101 3 -1.1052827246263952 1101 4 -0.5503853753721089 1101 5 -0.28323955131022416 1101 6 -2.167856425404029 1101 7 -0.6920410599330218 1101 8 6.692372750727316E-4 1101 9 -0.17460769988600333 1101 10 -9.255239268683649 1101 11 -3.6691933768377893 1101 12 -1.397854102753648 1101 13 -0.7940996029426373 1101 14 -0.5585272424938807 1101 15 -1.9053892762082256 1101 16 -0.2929024466789798 1101 17 -0.22818093997130262 1101 18 -0.17506138381571645 1101 19 -0.8021408196452526 1101 20 -0.22762350944919518 1101 21 -0.10569289217100675 1101 22 0.1492837689352271 1101 23 -0.12694880644809814 1101 24 -0.10418554285416803 1101 25 -0.2296804234573031 1102 1 -0.18838155057355976 1102 2 -0.3906755213328168 1102 3 -0.6652995193494426 1102 4 -0.18040452297906384 1102 5 -0.21868331332818514 1102 6 -1.0805826876451121 1102 7 -0.1831528381453147 1102 8 0.39493955929563285 1102 9 0.24573500982491225 1102 10 0.3265112769420142 1102 11 0.2220567347213631 1102 12 0.04261996278633973 1102 13 0.09353396378595846 1102 14 -0.053843786513098735 1102 15 0.3316576922225636 1102 16 0.29610147208973814 1102 17 -0.03906992441792312 1102 18 -0.18613007991236685 1102 19 0.15673442071400245 1102 20 0.09233219142623739 1102 21 0.09525759179287263 1102 22 0.0441767938514559 1102 23 -0.23512872904633042 1102 24 -3.6211364487144735 1102 25 -1.521338638673157 1103 1 0.1863438401735813 1103 2 -0.08893934113229562 1103 3 0.18225021487346993 1103 4 0.05247732093323876 1103 5 -0.303221340364761 1103 6 0.5962585648573331 1103 7 0.07433622824413533 1103 8 0.39580856306808343 1103 9 0.43214241016357846 1103 10 0.3345069238016265 1103 11 -0.0024375984806752626 1103 12 0.2841106291173148 1103 13 0.08012125602705567 1103 14 0.2377242373966908 1103 15 0.7065668325991581 1103 16 0.5042826959231803 1103 17 0.2754140132249603 1103 18 0.3095990101919526 1103 19 -1.1372091508915005 1103 20 -0.08839419826916384 1103 21 0.0035888609038536856 1103 22 0.06424408073234389 1103 23 0.14578120724026178 1103 24 1.5845057816202512 1103 25 0.7533528446837963 1104 1 -0.1543831120548731 1104 2 -0.07689625361926034 1104 3 -1.282581656486137 1104 4 -0.3599607886240612 1104 5 -0.2639803569772248 1104 6 -0.9686329287088498 1104 7 -0.2314299769320307 1104 8 0.44238766418854425 1104 9 0.35775922083416134 1104 10 0.6952422111746799 1104 11 0.5077011677185155 1104 12 0.5461312835258667 1104 13 0.4049207040729783 1104 14 0.5538964119721044 1104 15 0.8461396265276591 1104 16 0.413510378972326 1104 17 0.25822726411082186 1104 18 0.3123627686641939 1104 19 -0.2658535333430265 1104 20 0.14365582855086245 1104 21 0.35491221466293865 1104 22 0.36228233067102167 1104 23 0.5656013037125831 1104 24 1.942532146998694 1104 25 0.9933082975234271 1105 1 -0.2724675051048564 1105 2 -0.47125789442531385 1105 3 0.032068901703187436 1105 4 -0.32342850304757675 1105 5 -0.4757942860262041 1105 6 -0.6423754832435762 1105 7 -0.2721697591460419 1105 8 -0.018327335752637453 1105 9 -0.8608776757351393 1105 10 -0.3663154644677249 1105 11 -0.5124756020376964 1105 12 -0.391642468439656 1105 13 -0.479081005590146 1105 14 -0.7072487486225114 1105 15 -0.1932826171307056 1105 16 -0.8133140786213602 1105 17 -0.6042624916347847 1105 18 -0.8006063593684019 1105 19 -1.684773935976537 1105 20 -0.6832309768494795 1105 21 -0.7982594849225819 1105 22 -0.7476066588631696 1105 23 -0.9273326115336147 1105 24 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-2.3854287245515096 2214 25 -1.344955804961387 2215 1 0.5633134886928204 2215 2 0.19923472694775413 2215 3 0.6145526829821506 2215 4 0.6457381042098547 2215 5 0.9722463974176314 2215 6 1.496986699214066 2215 7 0.9897655560793552 2215 8 -0.26649159040438164 2215 9 0.2940497261802122 2215 10 0.0995955167351994 2215 11 -0.04750328674100501 2215 12 -0.1350421024787707 2215 13 0.2666474704441697 2215 14 0.07643034645602853 2215 15 0.07353613717646823 2215 16 0.5835752538564805 2215 17 -0.08899431834992555 2215 18 -0.6789718925068726 2215 19 0.4037101779941352 2215 20 -3.3775229375918556 2215 21 -1.8325386132702945 2215 22 -0.954006221611202 2215 23 -0.8710245390068181 2215 24 -0.8091394729571952 2215 25 -0.18131863964871542 2216 1 -0.6460066509808371 2216 2 -1.1603149835130786 2216 3 -0.2920740540449529 2216 4 -0.5247328739585129 2216 5 -0.3424526934469405 2216 6 -1.6285874587568792 2216 7 -9.953647023605132 2216 8 -10.935292416840735 2216 9 -6.219123451229169 2216 10 -3.7097604746049626 2216 11 -2.4536176591012193 2216 12 -2.319682805023593 2216 13 -2.078238169207772 2216 14 -2.090245714332873 2216 15 -3.175181103132264 2216 16 -2.2619632002898875 2216 17 -2.258510792098285 2216 18 -2.278782285239707 2216 19 -1.7599517555749422 2216 20 -2.644431202602199 2216 21 -5.247038394741452 2216 22 -3.6045319382518315 2216 23 -3.443558096813476 2216 24 -3.568917599190172 2216 25 -3.2061717910379244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38575.0 3 5.0 4 285.0 5 1599.0 6 2119.0 7 1697.0 8 1391.0 9 1257.0 10 1193.0 11 1176.0 12 1101.0 13 1180.0 14 1141.0 15 1173.0 16 1309.0 17 1442.0 18 1685.0 19 2167.0 20 2666.0 21 3572.0 22 4347.0 23 5237.0 24 6126.0 25 6998.0 26 7998.0 27 8514.0 28 9653.0 29 11372.0 30 13664.0 31 16846.0 32 21740.0 33 28454.0 34 38641.0 35 53422.0 36 78011.0 37 123132.0 38 157645.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.39369152657389 18.494377269898653 12.42204177146048 25.68988943206698 2 13.986088410809987 19.944049564787715 42.78353415181117 23.28632787259113 3 19.762475653830542 23.957238927719434 28.754251551997033 27.52603386645299 4 11.48744958712348 16.833265964204198 38.67256475794221 33.00671969073011 5 12.399467166489208 38.15774484444086 36.226906877167295 13.215881111902632 6 31.472953227477223 38.198269079464986 18.080798568603445 12.247979124454341 7 27.89614486852904 31.08482385525328 21.967808685351294 19.051222590866388 8 24.160922585468427 38.30955763342332 21.064524036829116 16.46499574427914 9 26.065243607930817 15.7095903061085 20.205226657594086 38.0199394283666 10 13.997669688172682 29.70782993888628 35.298755394732645 20.995744978208393 11 34.49153636439578 22.588072690820997 24.50482605691026 18.415564887872964 12 23.132956861696798 25.054602563206835 32.13683599789792 19.675604577198456 13 28.994219442478443 22.313164665392897 25.547907547530883 23.144708344597774 14 19.864581418978954 22.945673090796976 27.781768916605788 29.40797657361828 15 22.45905324293456 32.37026439211684 22.91621102767855 22.254471337270054 16 21.237237729842374 28.682414267618086 28.90583943157965 21.174508570959894 17 20.935711063553804 29.403223332173933 28.4155063815051 21.245559222767156 18 20.818385888599643 28.235406686937477 30.476991819786214 20.469215604676663 19 23.357601036529147 27.238205759541646 27.31886182294855 22.085331380980662 20 22.870057320951766 28.137691791287338 28.595621493382424 20.396629394378476 21 22.11703631925955 27.44364842021169 27.308322613079067 23.130992647449695 22 22.086573511543133 29.41190765492102 27.561968408262455 20.93955042527339 23 21.415661186648684 28.7299967192797 28.25398850532813 21.600353588743484 24 22.86154308808989 27.52081918934641 28.71658411894163 20.901053603622074 25 21.938728241174584 28.057702559647396 28.483424487136453 21.520144712041567 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 181.0 1 181.0 2 243.5 3 306.0 4 306.0 5 306.0 6 443.0 7 580.0 8 580.0 9 580.0 10 854.5 11 1129.0 12 1129.0 13 1129.0 14 1966.0 15 2803.0 16 2803.0 17 2803.0 18 5139.0 19 7475.0 20 7475.0 21 7475.0 22 11939.0 23 16403.0 24 16403.0 25 16403.0 26 23844.0 27 31285.0 28 31285.0 29 31285.0 30 41762.5 31 52240.0 32 52240.0 33 52240.0 34 60098.5 35 67957.0 36 67957.0 37 67957.0 38 73525.5 39 79094.0 40 79094.0 41 79094.0 42 82661.0 43 86228.0 44 86228.0 45 86228.0 46 87659.5 47 89091.0 48 89091.0 49 89091.0 50 84344.0 51 79597.0 52 79597.0 53 79597.0 54 68950.5 55 58304.0 56 58304.0 57 58304.0 58 48982.0 59 39660.0 60 39660.0 61 39660.0 62 31964.0 63 24268.0 64 24268.0 65 24268.0 66 18435.5 67 12603.0 68 12603.0 69 12603.0 70 9171.0 71 5739.0 72 5739.0 73 5739.0 74 4019.5 75 2300.0 76 2300.0 77 2300.0 78 1587.0 79 874.0 80 874.0 81 874.0 82 583.0 83 292.0 84 292.0 85 292.0 86 194.5 87 97.0 88 97.0 89 97.0 90 57.5 91 18.0 92 18.0 93 18.0 94 12.0 95 6.0 96 6.0 97 6.0 98 4.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1958899554008683 2 0.12269696431310201 3 0.1263414285996298 4 0.07091520090868643 5 0.025207544648483828 6 0.02353716518382526 7 0.2690829464886346 8 0.26877924113142393 9 0.02110752232614007 10 0.038266875008541715 11 0.019437142861481505 12 0.02110752232614007 13 0.01807046875403359 14 0.019740848218692153 15 0.017766763396822937 16 0.022322343754982665 17 0.022170491076377342 18 0.01746305803961229 19 0.027637187506169014 20 0.020500111611718778 21 0.01852602678984956 22 0.020196406254508126 23 0.02141122768335072 24 0.019133437504270857 25 0.01852602678984956 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 658533.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.37624728601413 #Duplication Level Percentage of deduplicated Percentage of total 1 94.14217925362021 81.31648155254969 2 4.481868449970759 7.742539550761183 3 0.6691532339883713 1.7339683563364694 4 0.23436096035762705 0.8097288106415259 5 0.11838571592563277 0.511285693696214 6 0.07463729894906365 0.3868133874470691 7 0.05159683206407683 0.311971850787915 8 0.03228600324219501 0.2230995039939912 9 0.02917467444755826 0.2267999005114125 >10 0.13683714713467124 2.2254571644408667 >50 0.01599040317503733 0.9563564429040353 >100 0.012124310021961793 2.217139574194785 >500 7.028585514057769E-4 0.4773346082905646 >1k 7.028585514057769E-4 0.8610236034443158 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1792 0.2721200000607411 No Hit CTGTAGGACGTGGAATATGGCAAGA 1437 0.21821229915585097 No Hit GTCCTACAGTGTGCATTTCTCATTT 1266 0.19224549111434053 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1178 0.17888245539707198 No Hit CTTTAGGACGTGAAATATGGCGAGG 928 0.14091928574574092 No Hit CTGTAGGACCTGGAATATGGCGAGA 849 0.1289229241359203 No Hit GTATCAACGCAGAGTACTTTTTTTT 752 0.11419321431120386 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.5185267860532426E-4 0.0 0.0 0.0 6 0.0 1.5185267860532426E-4 0.0 0.0 0.0 7 0.0 1.5185267860532426E-4 0.0 0.0 0.0 8 0.0 1.5185267860532426E-4 0.0 0.0 0.0 9 0.0 1.5185267860532426E-4 0.0 0.0 0.0 10 0.0 1.5185267860532426E-4 0.0 0.0 0.0 11 0.0 1.5185267860532426E-4 0.0 0.0 0.0 12 0.0 1.5185267860532426E-4 0.0 0.0 0.0 13 0.0 1.5185267860532426E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAGG 80 1.2312557E-7 14.299469 1 TAGGACC 275 0.0 13.845014 4 TAGACTC 50 0.0014778964 13.323793 5 GTCTTAG 105 1.891567E-8 12.710639 1 TAAATGC 75 1.4463647E-5 12.691261 4 GTAGGAC 825 0.0 12.243761 3 TGGGACC 70 1.0695042E-4 12.238002 4 CTGTAGG 895 0.0 12.036053 1 TAGGACT 95 1.0127151E-6 12.0233 4 GTCCTAC 675 0.0 12.004492 1 TCCAACG 80 2.908782E-5 11.855585 18 TGTAGGA 945 0.0 11.596555 2 TAGGAGA 125 1.7716957E-8 11.422136 4 TTAGACA 75 2.0333656E-4 11.422135 4 TGCACTT 75 2.1020568E-4 11.381361 12 AGGACCT 430 0.0 11.2876005 5 ATAGCAC 85 5.1474664E-5 11.210991 3 GAACAGT 85 5.2159274E-5 11.196466 6 GGACCTG 460 0.0 11.172126 6 TTAATCT 60 0.005785381 11.104855 4 >>END_MODULE