FastQCFastQC Report
Thu 26 May 2016
SRR1926962_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926962_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658533
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA17360.2636162500588429No Hit
GTCCTACAGTGGACATTTCTAAATT17020.25845325898626187No Hit
GTATCAACGCAGAGTACTTTTTTTT13680.2077344643320836No Hit
CTTTAGGACGTGAAATATGGCGAGG12140.18434915182686365No Hit
GTCCTACAGTGTGCATTTCTCATTT12050.18298247771941575No Hit
GTCCTAAAGTGTGTATTTCTCATTT11430.17356761164588563No Hit
CTGTAGGACCTGGAATATGGCGAGA10110.15352305806998284No Hit
GGTATCAACGCAGAGTACTTTTTTT8740.1327192411010534No Hit
GTACATGGGAAGCAGTGGTATCAAC7400.11237098216793995No Hit
TATCAACGCAGAGTACTTTTTTTTT6980.10599316966651634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA3000.013.9821291
TAGGACC3550.013.1475624
TCCAACG655.5060395E-513.13775818
TAGGACT1102.6266207E-912.9890114
GGCGAGG3200.012.750010519
TAGGATA751.4357382E-512.7003674
GGTGCCC604.136812E-412.6482918
TATTAAG550.002985971512.1332522
GATAGTG550.003096962612.07428557
TGTAGGA9650.011.8548432
GTAGGAC9450.011.7940963
TACACTG3750.011.6843375
CCTGTAC1156.782648E-811.596873
TCCTAAA3550.011.547342
GGACGTG7700.011.5045536
AGGACCT5400.011.4656095
CTGTAGG9650.011.45968151
AGGACGT8200.011.2677025
CTAAAGT3650.011.2215564
AATCCCG855.3784563E-511.16280119