##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926962_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 658533 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.976329204459002 31.0 30.0 33.0 26.0 34.0 2 30.28014237707146 31.0 30.0 34.0 26.0 34.0 3 30.341897824406672 31.0 30.0 34.0 26.0 34.0 4 33.964480139947426 35.0 35.0 37.0 30.0 37.0 5 34.00663748058184 37.0 35.0 37.0 30.0 37.0 6 33.97614546271789 37.0 35.0 37.0 30.0 37.0 7 33.97183740222586 37.0 35.0 37.0 30.0 37.0 8 34.00340301852754 37.0 35.0 37.0 30.0 37.0 9 35.55483931708813 39.0 35.0 39.0 30.0 39.0 10 35.45018548804692 39.0 35.0 39.0 30.0 39.0 11 35.501391729799415 39.0 35.0 39.0 30.0 39.0 12 35.40233670901838 39.0 35.0 39.0 30.0 39.0 13 35.42774925478298 39.0 35.0 39.0 30.0 39.0 14 36.52881632355554 40.0 37.0 41.0 30.0 41.0 15 36.529498142082474 40.0 37.0 41.0 30.0 41.0 16 36.5097664050245 40.0 37.0 41.0 30.0 41.0 17 36.48600753492991 40.0 37.0 41.0 30.0 41.0 18 36.46132995612976 40.0 37.0 41.0 30.0 41.0 19 36.52683160904617 40.0 37.0 41.0 30.0 41.0 20 36.49759237578071 40.0 37.0 41.0 30.0 41.0 21 36.44343563648291 40.0 37.0 41.0 30.0 41.0 22 36.35493741391851 40.0 37.0 41.0 30.0 41.0 23 36.290841916805995 40.0 37.0 41.0 30.0 41.0 24 36.2298244734888 40.0 37.0 41.0 30.0 41.0 25 36.10832562680989 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04099182750211483 1101 2 0.07169608520541004 1101 3 0.3266410125328534 1101 4 0.15774374767885746 1101 5 0.16553004459984066 1101 6 0.26400510880273487 1101 7 0.21292940162400242 1101 8 0.13093487380303515 1101 9 0.19205357504762333 1101 10 0.3852795455957434 1101 11 0.3590521589187432 1101 12 0.30784635736279853 1101 13 0.17981871011278372 1101 14 0.3495623841194515 1101 15 0.33812142995733296 1101 16 0.411473925171137 1101 17 0.39942122510500866 1101 18 0.4212861845917075 1101 19 0.24212245253956866 1101 20 0.2468975414732526 1101 21 0.33009346151908403 1101 22 0.22478691918314553 1101 23 0.2216341135387978 1101 24 0.2350624345904606 1101 25 0.23368216510997541 1102 1 -0.5669749678719214 1102 2 -0.37811446707323526 1102 3 -0.08188859729408904 1102 4 -0.25007796862825415 1102 5 -0.21356620457868303 1102 6 -0.20151702858946408 1102 7 -0.030407474557506475 1102 8 -0.21305066813103934 1102 9 -0.10733467513696127 1102 10 -0.03977501697068675 1102 11 0.10340118084923944 1102 12 0.11527903648622839 1102 13 0.12715873429069546 1102 14 -0.011392318808674418 1102 15 -0.05339033851888786 1102 16 0.11145080200171975 1102 17 0.1690536072782649 1102 18 0.061242210002859565 1102 19 -0.059208955215211745 1102 20 -0.014343564219913674 1102 21 0.11975375376349717 1102 22 0.10053639384096158 1102 23 0.21906216802834422 1102 24 0.20374473846947438 1102 25 0.08066154897892375 1103 1 -0.7822828347925217 1103 2 -0.6842129243885005 1103 3 -0.6606836870167001 1103 4 -0.6947063883002258 1103 5 -0.5153673570238695 1103 6 -0.3606624811414463 1103 7 -0.496039187510128 1103 8 -0.4747680063806001 1103 9 -0.306722311730951 1103 10 -0.23979105699358882 1103 11 -0.22987293299025424 1103 12 -0.06860865898886459 1103 13 -0.18668360243608362 1103 14 -0.22733061954082956 1103 15 -0.2538358044714002 1103 16 -0.38906999114695395 1103 17 -0.11026816370269188 1103 18 -0.1305416435821911 1103 19 -0.12123917543051732 1103 20 -0.2749787085192068 1103 21 -0.236889049139144 1103 22 -0.17689407570532723 1103 23 -0.3044983921948372 1103 24 -0.14065891784359508 1103 25 -0.13614575552568198 1104 1 -0.007654775664843072 1104 2 0.06486731832771753 1104 3 0.31759707973684215 1104 4 0.21255715182312684 1104 5 0.01626332029636046 1104 6 0.04466981794268321 1104 7 -0.06864766901827579 1104 8 0.10119945863871038 1104 9 -0.06616066655686126 1104 10 -0.10123822154888273 1104 11 0.02532107846757725 1104 12 0.14244398203560138 1104 13 0.09157064340282517 1104 14 0.30413355429279676 1104 15 0.019484155408235893 1104 16 0.14322171498535852 1104 17 0.03687904332594627 1104 18 0.12544001909969182 1104 19 -0.11058893444911888 1104 20 -0.2496382736958509 1104 21 -0.03446667266578629 1104 22 0.005907809074258807 1104 23 0.29860515303329294 1104 24 0.2288531970298493 1104 25 0.19963394939424717 1105 1 -0.19575105008929938 1105 2 -0.3797235192895769 1105 3 -0.07986430579611437 1105 4 -0.09148251549838449 1105 5 -0.11979998429423944 1105 6 -0.385208505803206 1105 7 -0.6534740166523889 1105 8 -0.7761929913948151 1105 9 -0.41398888462517647 1105 10 -0.44102592530661155 1105 11 -0.21537282517172684 1105 12 -0.2540763045290362 1105 13 -0.10968337097246206 1105 14 -0.2849571480415207 1105 15 -0.11956909902963275 1105 16 -0.2647819396156379 1105 17 -0.3071220389378695 1105 18 -0.3787557138007358 1105 19 -0.3956046782506135 1105 20 -0.17029186692935383 1105 21 -0.18518759368797788 1105 22 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0.38714667386661716 1107 10 0.3864955698773187 1107 11 0.47756296694255695 1107 12 0.46262387356023993 1107 13 0.33990587473329015 1107 14 0.5516928586607648 1107 15 0.48550357288396384 1107 16 0.5353118923676661 1107 17 0.25488749745553463 1107 18 0.1479998060093024 1107 19 0.41172379113909585 1107 20 0.6060624732786124 1107 21 0.4932099858298429 1107 22 0.5667582404664842 1107 23 0.5417617474473886 1107 24 0.6632313938682373 1107 25 0.3560025340030961 1108 1 0.7482026937074586 1108 2 0.5361079020363526 1108 3 0.5844101952130814 1108 4 0.7453786871398975 1108 5 0.607217111639649 1108 6 0.671780815707308 1108 7 0.7251667042175285 1108 8 0.6367258532351769 1108 9 0.4288527321567841 1108 10 0.3309132814834541 1108 11 0.4367079564223957 1108 12 0.5965686026307893 1108 13 0.6331588208493244 1108 14 0.4108180343898624 1108 15 0.4086969833965739 1108 16 0.5061394466903621 1108 17 0.42465926191721337 1108 18 0.5761381626002091 1108 19 0.5165809906069541 1108 20 0.6537286964946674 1108 21 0.6437265586170753 1108 22 0.7386396976263256 1108 23 0.4705916982904199 1108 24 0.44246752660149724 1108 25 0.5507584946397799 1109 1 0.6584812283304373 1109 2 0.38670338565809814 1109 3 0.5459593412017405 1109 4 0.8965350936045837 1109 5 0.6564281063633999 1109 6 0.821788625788578 1109 7 0.7187128646038659 1109 8 0.7039056469180807 1109 9 0.6294567741310644 1109 10 0.6037694609413506 1109 11 0.5731916988262711 1109 12 0.9039500180045792 1109 13 0.7106320210464929 1109 14 0.8641488236009138 1109 15 0.709089545206119 1109 16 0.8145862132699548 1109 17 0.8484810386686163 1109 18 0.6022930491389005 1109 19 0.8110668861613846 1109 20 0.8279711419947944 1109 21 0.8224361835367517 1109 22 0.8191218836259822 1109 23 0.7930666803085273 1109 24 0.686806153018658 1109 25 0.780800463304054 1110 1 0.4288015103606959 1110 2 0.25523885926254763 1110 3 0.3170967578316173 1110 4 0.4617353966938822 1110 5 0.4694322459636311 1110 6 0.6226445148703306 1110 7 0.4620201757648772 1110 8 0.5646646315140913 1110 9 0.4345756562470555 1110 10 0.34135408784263177 1110 11 0.2714048795546944 1110 12 0.5651215915919394 1110 13 0.1429282149724571 1110 14 0.266872788403802 1110 15 0.09878140137215041 1110 16 0.04093798148890215 1110 17 0.10010274008178044 1110 18 0.16153210853836697 1110 19 0.26390295561599686 1110 20 0.2688198285925978 1110 21 0.26122923734423864 1110 22 0.29333141558335285 1110 23 0.3417442293068973 1110 24 0.2205138692736952 1110 25 0.249365002770098 1111 1 0.49363766092023553 1111 2 0.43477597347142805 1111 3 0.3585825246201253 1111 4 0.47022339147888204 1111 5 0.41794500837058735 1111 6 0.4419135068608 1111 7 0.3299473763880627 1111 8 0.2853488643079416 1111 9 0.25937559959384515 1111 10 0.2212057000078218 1111 11 0.32998829505334015 1111 12 0.31227395059898555 1111 13 0.5195268069948895 1111 14 0.3283306280643217 1111 15 0.1227346334197108 1111 16 0.3835864101793973 1111 17 0.446931158930596 1111 18 0.3584492296110042 1111 19 0.37599823469661686 1111 20 0.3414575984897681 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0.15559835097867492 2213 2 0.0666371869210387 2213 3 0.23064226080801475 2213 4 0.03082950643840121 2213 5 5.396510547726052E-4 2213 6 -0.18818632848083894 2213 7 -0.2249369502166374 2213 8 -0.2009296056248644 2213 9 -0.289175299211216 2213 10 -0.4615435565184285 2213 11 -0.3090852415858336 2213 12 -0.14886631751792123 2213 13 -0.1988015698467578 2213 14 -0.38773773206037276 2213 15 -0.28070346185255346 2213 16 -0.2783240572853316 2213 17 -0.4205583212296844 2213 18 -0.34257925736161354 2213 19 -0.5510624162599456 2213 20 -0.5503611409948732 2213 21 -0.616298384085816 2213 22 -0.5516063927690951 2213 23 -0.46909819535666486 2213 24 -0.5103364306823011 2213 25 -0.24581717127386327 2214 1 0.07737757369735832 2214 2 0.11737023313530415 2214 3 0.1468230050632009 2214 4 0.040465274905315596 2214 5 0.18492381842379046 2214 6 0.11706163356313937 2214 7 -0.040538192301262654 2214 8 -0.0043365840257081345 2214 9 0.1312650263124553 2214 10 -0.18053735811103166 2214 11 -0.11941071376165979 2214 12 -0.15332759163309362 2214 13 -0.237137801263124 2214 14 -0.32983300317847153 2214 15 -0.2197656158634942 2214 16 -0.44166694027430253 2214 17 -0.22708338521352545 2214 18 -0.01844597872852205 2214 19 -0.15605083823227517 2214 20 -0.14147481165019116 2214 21 -0.1925520124113902 2214 22 -0.032106556698174415 2214 23 -0.3514305886010689 2214 24 -0.3610511099735092 2214 25 -0.26928992729074963 2215 1 0.3264204762527534 2215 2 0.3413382425506839 2215 3 0.36591882673400633 2215 4 0.3850983642335635 2215 5 0.4558279713151805 2215 6 0.3940148966824779 2215 7 0.16768042359380075 2215 8 0.14923653737113796 2215 9 -0.11504161626178444 2215 10 0.032014164044873894 2215 11 -0.12859969715670871 2215 12 -0.010617261255347898 2215 13 -0.018858533159672675 2215 14 -0.08496190078028576 2215 15 0.008826138726121258 2215 16 0.08665932841506674 2215 17 0.3762386226433705 2215 18 0.05980325580549817 2215 19 0.043786020639622336 2215 20 -0.03098909370628178 2215 21 0.10192535514879353 2215 22 -0.11112401296846741 2215 23 -0.027779449453731786 2215 24 -0.015049466691252178 2215 25 -0.053441665498418445 2216 1 -0.34075686105875747 2216 2 -0.03976312434030049 2216 3 -1.8370542743519849 2216 4 -1.6324218277391793 2216 5 -0.6696907459343748 2216 6 -0.47979555333910184 2216 7 -0.5346195347332525 2216 8 -0.406025387697035 2216 9 -0.46956377730413834 2216 10 -0.5708750367494062 2216 11 -0.47365812705699994 2216 12 -0.393421582305173 2216 13 -0.3844743825531012 2216 14 -0.5369664062629056 2216 15 -0.41870128826075614 2216 16 -0.5808377170564896 2216 17 -0.6994804666134229 2216 18 -0.6616034231382031 2216 19 -0.6358858072502045 2216 20 -0.5132688531305121 2216 21 -0.46511310315563037 2216 22 -0.5275762318447335 2216 23 -0.5991330439067042 2216 24 -0.756990464827517 2216 25 -0.8714103729072349 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14062.0 3 10.0 4 193.0 5 1383.0 6 1940.0 7 1471.0 8 1134.0 9 1009.0 10 887.0 11 793.0 12 798.0 13 730.0 14 810.0 15 764.0 16 782.0 17 791.0 18 920.0 19 1104.0 20 1437.0 21 1880.0 22 2371.0 23 2852.0 24 3462.0 25 4150.0 26 4893.0 27 5527.0 28 6409.0 29 7799.0 30 10094.0 31 13329.0 32 17156.0 33 23028.0 34 31212.0 35 44370.0 36 65055.0 37 115075.0 38 268853.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.892588330264964 19.350640860947387 10.490781970455481 28.265988838332166 2 14.049546577978555 20.60220159723721 40.9569439082151 24.391307916569133 3 19.185340997972464 24.142702013106838 28.87376886101134 27.798188127909363 4 11.871083149971223 17.254716164565785 38.21023395942193 32.66396672604107 5 12.656814968587652 38.805677761089996 35.721998059222564 12.81550921109979 6 32.020441497336634 38.119522422262584 17.49122368629554 12.368812394105243 7 28.602086519637247 31.554774557765064 21.292888853112846 18.55025006948484 8 24.684764038118377 39.17612665628915 19.429135502486808 16.70997380310566 9 25.95254764446726 15.848837686093997 19.79373427227623 38.404880397162515 10 14.242890399704788 30.055686237587526 34.44073067875859 21.26069268394909 11 34.52182813268783 22.748850431581698 24.355209048333506 18.374112387396966 12 23.438800240535006 25.055730017675675 31.81318447698821 19.692285264801104 13 29.26583339534022 22.257182295692367 25.529475543218293 22.947508765749117 14 20.045920250010248 23.205063375715415 27.419278912370377 29.32973746190396 15 22.370738877955148 32.45483757319533 22.844838059139395 22.329585489710134 16 21.143759585744007 29.099053953502498 28.63954565471581 21.11764080603769 17 20.90923447911415 29.804170488298215 28.116932814198854 21.16966221838878 18 20.86647533141998 28.28481618149515 30.397248416928612 20.45146007015626 19 22.964142918101047 27.500451763090876 27.562863403545162 21.972541915262912 20 23.024042980643223 28.04037483542866 28.49760602798054 20.437976155947574 21 22.05641144023522 27.164028138896313 27.428437780963723 23.351122639904744 22 22.318243732660324 29.284333511000966 27.376000898967856 21.021421857370854 23 21.383471114539613 28.76838179465842 28.11905875492763 21.729088335874337 24 22.623771322014235 27.382682416826494 28.89422397966389 21.099322281495382 25 21.900598027632583 28.262804139116593 28.409046041142116 21.427551792108705 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 192.0 1 192.0 2 197.0 3 202.0 4 202.0 5 202.0 6 355.5 7 509.0 8 509.0 9 509.0 10 815.5 11 1122.0 12 1122.0 13 1122.0 14 1866.0 15 2610.0 16 2610.0 17 2610.0 18 4779.5 19 6949.0 20 6949.0 21 6949.0 22 11380.5 23 15812.0 24 15812.0 25 15812.0 26 23289.0 27 30766.0 28 30766.0 29 30766.0 30 41162.0 31 51558.0 32 51558.0 33 51558.0 34 59094.5 35 66631.0 36 66631.0 37 66631.0 38 72201.0 39 77771.0 40 77771.0 41 77771.0 42 81843.5 43 85916.0 44 85916.0 45 85916.0 46 88222.5 47 90529.0 48 90529.0 49 90529.0 50 85744.0 51 80959.0 52 80959.0 53 80959.0 54 70276.5 55 59594.0 56 59594.0 57 59594.0 58 50012.5 59 40431.0 60 40431.0 61 40431.0 62 32451.5 63 24472.0 64 24472.0 65 24472.0 66 18788.0 67 13104.0 68 13104.0 69 13104.0 70 9449.5 71 5795.0 72 5795.0 73 5795.0 74 4047.5 75 2300.0 76 2300.0 77 2300.0 78 1615.0 79 930.0 80 930.0 81 930.0 82 603.0 83 276.0 84 276.0 85 276.0 86 184.5 87 93.0 88 93.0 89 93.0 90 51.5 91 10.0 92 10.0 93 10.0 94 6.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11328209823957189 2 0.0982486830576448 3 0.01488156250332178 4 0.0 5 0.005162991072581025 6 0.3372647991824252 7 0.017614910718217615 8 0.008807455359108807 9 0.009414866073530103 10 0.0033407589293171338 11 0.0037963169651331063 12 1.5185267860532426E-4 13 6.07410714421297E-4 14 0.0 15 0.0031889062507118094 16 4.5555803581597275E-4 17 1.5185267860532426E-4 18 4.5555803581597275E-4 19 3.037053572106485E-4 20 6.07410714421297E-4 21 0.012907477681452562 22 0.0 23 1.5185267860532426E-4 24 0.0 25 0.005314843751186349 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 658533.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.76427138990239 #Duplication Level Percentage of deduplicated Percentage of total 1 92.51409647525296 77.493758845447 2 5.790282530710539 9.700375946532967 3 0.8725390451295614 2.192627921235566 4 0.2787613925546869 0.9340097975591164 5 0.14191088299815713 0.5943530858319158 6 0.0921938287581755 0.46335293355444 7 0.05889896039715092 0.3453539952303044 8 0.032637193952333325 0.21870646173025038 9 0.029272866800930185 0.22068183231662217 >10 0.15485710521135618 2.413252430207936 >50 0.017820561756433255 1.0115465238480543 >100 0.014365253933866978 2.3712527854106167 >500 0.001091031943312487 0.61215730599803 >1k 0.0012728706005312351 1.4285701350971693 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 1736 0.2636162500588429 No Hit GTCCTACAGTGGACATTTCTAAATT 1702 0.25845325898626187 No Hit GTATCAACGCAGAGTACTTTTTTTT 1368 0.2077344643320836 No Hit CTTTAGGACGTGAAATATGGCGAGG 1214 0.18434915182686365 No Hit GTCCTACAGTGTGCATTTCTCATTT 1205 0.18298247771941575 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1143 0.17356761164588563 No Hit CTGTAGGACCTGGAATATGGCGAGA 1011 0.15352305806998284 No Hit GGTATCAACGCAGAGTACTTTTTTT 874 0.1327192411010534 No Hit GTACATGGGAAGCAGTGGTATCAAC 740 0.11237098216793995 No Hit TATCAACGCAGAGTACTTTTTTTTT 698 0.10599316966651634 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAA 300 0.0 13.982129 1 TAGGACC 355 0.0 13.147562 4 TCCAACG 65 5.5060395E-5 13.137758 18 TAGGACT 110 2.6266207E-9 12.989011 4 GGCGAGG 320 0.0 12.7500105 19 TAGGATA 75 1.4357382E-5 12.700367 4 GGTGCCC 60 4.136812E-4 12.648291 8 TATTAAG 55 0.0029859715 12.133252 2 GATAGTG 55 0.0030969626 12.0742855 7 TGTAGGA 965 0.0 11.854843 2 GTAGGAC 945 0.0 11.794096 3 TACACTG 375 0.0 11.684337 5 CCTGTAC 115 6.782648E-8 11.59687 3 TCCTAAA 355 0.0 11.54734 2 GGACGTG 770 0.0 11.504553 6 AGGACCT 540 0.0 11.465609 5 CTGTAGG 965 0.0 11.4596815 1 AGGACGT 820 0.0 11.267702 5 CTAAAGT 365 0.0 11.221556 4 AATCCCG 85 5.3784563E-5 11.162801 19 >>END_MODULE