FastQCFastQC Report
Thu 26 May 2016
SRR1926961_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926961_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647840
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15550.24002840207458634No Hit
GTCCTACAGTGTGCATTTCTCATTT13750.21224376389231908No Hit
CTGTAGGACGTGGAATATGGCAAGA12330.1903247715485305No Hit
GTCCTAAAGTGTGTATTTCTCATTT11230.17334527043714498No Hit
CTGTAGGACCTGGAATATGGCGAGA8450.13043344035564336No Hit
CTTTAGGACGTGAAATATGGCGAGG8360.12904420844653No Hit
GTATCAACGCAGAGTACTTTTTTTT8220.1268831810323537No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCCA456.7086314E-414.79114158
TAGGCTA400.00521627914.2739345
GAGGTAA400.00533265114.226559
GTATAAC500.001457612513.3481881
CTAAGAC751.4260937E-512.7086153
ACAACTG1155.167749E-912.4121165
TAGGACC2550.012.3185844
GTCCTAC10500.012.1677361
TCTAGGG550.002990126412.1309513
TCTAGAC550.002990126412.1309513
GACGTGG3700.012.0878377
AGGACGT5850.011.8746125
ATCTAGA657.7727815E-411.7346711
GTTTAGA657.7727815E-411.7346711
CTAACAC657.793057E-411.7310293
CTTAGAC657.793057E-411.7310293
GTAGGAC7000.011.710083
CTGTAGG7700.011.6394211
GTCCTAA2900.011.5070581
TGTAGGA7750.011.191782
Produced by FastQC (version 0.11.5)