##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926961_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 647840 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.385451654729565 31.0 28.0 31.0 22.0 34.0 2 28.42551092862435 31.0 28.0 31.0 22.0 34.0 3 28.0036891825142 31.0 28.0 31.0 16.0 34.0 4 31.870917201778216 35.0 32.0 37.0 19.0 37.0 5 31.692070572980985 35.0 32.0 37.0 19.0 37.0 6 31.30158835514942 35.0 32.0 37.0 17.0 37.0 7 31.165133366263277 35.0 32.0 37.0 17.0 37.0 8 31.280292973573722 35.0 32.0 37.0 17.0 37.0 9 33.459650530995305 37.0 33.0 39.0 18.0 39.0 10 33.62305044455421 38.0 34.0 39.0 19.0 39.0 11 33.78622345023462 38.0 34.0 39.0 23.0 39.0 12 33.66213571252161 38.0 34.0 39.0 22.0 39.0 13 33.76312206717708 38.0 34.0 39.0 23.0 39.0 14 34.804215547048656 39.0 35.0 41.0 23.0 41.0 15 34.40450265497654 39.0 34.0 41.0 17.0 41.0 16 34.48788898493455 39.0 34.0 41.0 18.0 41.0 17 34.714191775747096 39.0 34.0 41.0 22.0 41.0 18 34.75734594961719 39.0 35.0 41.0 22.0 41.0 19 34.13033773771301 39.0 34.0 41.0 16.0 41.0 20 34.42292541368239 39.0 34.0 41.0 18.0 41.0 21 34.40640590269202 39.0 34.0 41.0 18.0 41.0 22 34.433775314892564 39.0 34.0 41.0 18.0 41.0 23 34.43135959496172 39.0 34.0 41.0 17.0 41.0 24 33.134755495183995 39.0 32.0 41.0 2.0 41.0 25 33.71178531736231 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.0016380059324241358 1101 2 -0.042198847514690385 1101 3 -0.8580482921063997 1101 4 -0.07616714574894345 1101 5 -0.28400431749517097 1101 6 -2.1939030020569312 1101 7 -0.8655638021946679 1101 8 0.08061742021823903 1101 9 0.001220928778884911 1101 10 -9.284354860417846 1101 11 -3.8340063713912187 1101 12 -1.2788246212980425 1101 13 -0.33090992868992686 1101 14 0.045075556336414024 1101 15 -1.4888083402944474 1101 16 0.046222119513110727 1101 17 0.1314871214311637 1101 18 0.18370774041065374 1101 19 -0.23786805022563584 1101 20 0.23211296468792852 1101 21 -0.016374970908522357 1101 22 0.3863773222931073 1101 23 0.049350167435513015 1101 24 -0.018292204409817714 1101 25 0.20133618369784045 1102 1 -0.45245028991268654 1102 2 -0.683272796929586 1102 3 -1.0131316605787646 1102 4 -0.752439079783187 1102 5 -0.7354191712215439 1102 6 -1.0797516608603637 1102 7 -0.681491003470672 1102 8 -0.19731595186688367 1102 9 -0.2184046599122098 1102 10 -0.029202364216637022 1102 11 -0.45987653420265673 1102 12 -0.44668853133924813 1102 13 -0.44409499149263354 1102 14 -0.6625109153346997 1102 15 -0.23805154803675066 1102 16 -0.20384123858470105 1102 17 -0.4076131827955507 1102 18 -0.5555383683237025 1102 19 -0.19719592595533442 1102 20 -0.19088618658316392 1102 21 -0.3925128553843322 1102 22 -0.5711678992121989 1102 23 -0.7006878474231755 1102 24 -4.166950252937269 1102 25 -2.166223823161012 1103 1 -0.0567755005254682 1103 2 -0.407264189394418 1103 3 -0.046057540257379515 1103 4 -0.20240263746331166 1103 5 -0.31915149290597 1103 6 0.40818988414238433 1103 7 0.2389456112598225 1103 8 0.6248596058955478 1103 9 0.18766891566959742 1103 10 0.3703841535328891 1103 11 -0.2167309934322006 1103 12 -0.24334905028221243 1103 13 -0.040261021185813206 1103 14 -0.05134559499425251 1103 15 0.029424250039220112 1103 16 0.07811077018311607 1103 17 -0.029198948956072 1103 18 -0.361226619168157 1103 19 -2.1085896470465 1103 20 -0.6965323094560532 1103 21 -0.4349245680434066 1103 22 -0.12728094946144353 1103 23 -0.1833606885270811 1103 24 1.1666697094399652 1103 25 0.3032615549673139 1104 1 -0.16133119099312765 1104 2 -0.11775613121936956 1104 3 -1.5764582292005898 1104 4 -0.5569997822002222 1104 5 -0.458788727932955 1104 6 -1.8122676640114328 1104 7 -0.28732569757180926 1104 8 0.294945670307456 1104 9 0.21186751103848422 1104 10 0.31993083104006814 1104 11 0.30573508092873425 1104 12 0.23759107905510746 1104 13 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1.834158868277001 1110 9 1.4061998184848221 1110 10 1.339444001030877 1110 11 1.2863078456275616 1110 12 1.3016433992917271 1110 13 1.1614556635963424 1110 14 1.314694496938408 1110 15 1.8006670833573466 1110 16 1.4914665197080978 1110 17 1.1243360609590525 1110 18 1.2282428182006413 1110 19 -0.9554290469950573 1110 20 0.7648184285773496 1110 21 0.733335846981106 1110 22 1.0677471779499186 1110 23 1.0395283036154979 1110 24 -0.30700232826238505 1110 25 0.3187544484399325 1111 1 0.5042561520275513 1111 2 0.34482338587462635 1111 3 0.8803646737150785 1111 4 0.8572562566155746 1111 5 0.8218367484223847 1111 6 0.8047511112385131 1111 7 0.9828555789857774 1111 8 1.2547918417804382 1111 9 0.5743359408218396 1111 10 0.5672340993981209 1111 11 0.33026594875475723 1111 12 0.37230959975072153 1111 13 0.11548269333833616 1111 14 0.18767673210798108 1111 15 -0.4905208189399488 1111 16 -0.2887419987560449 1111 17 -0.14122844956153102 1111 18 0.23410486451747659 1111 19 0.9487797651026426 1111 20 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0.37600817883355475 2215 2 -0.09441045051328345 2215 3 0.4070557416230258 2215 4 0.37635641663820607 2215 5 0.5839423279960698 2215 6 0.9068570444533783 2215 7 0.9008391169889407 2215 8 -0.7548050956869616 2215 9 -0.13221260969137916 2215 10 -0.3148796139226633 2215 11 -0.439346548924469 2215 12 -0.5440524934514102 2215 13 -0.3170929637711808 2215 14 -0.17044773190460916 2215 15 -0.2187696762368958 2215 16 -0.07431520690040117 2215 17 -0.5034003368560889 2215 18 -1.229264098164542 2215 19 -0.08079082609699384 2215 20 -3.8134624113176656 2215 21 -2.531248816422451 2215 22 -1.3711706769128185 2215 23 -1.2999091653495327 2215 24 -1.0081639229279133 2215 25 -0.7691968887240037 2216 1 -0.9696448049773245 2216 2 -1.4398449827899178 2216 3 -0.6419519126477873 2216 4 -0.8833756838833651 2216 5 -0.8165775527869705 2216 6 -2.013184613746173 2216 7 -10.192377031490363 2216 8 -11.567156921196684 2216 9 -6.506120352020176 2216 10 -4.13998291271017 2216 11 -3.2186800177316215 2216 12 -2.8777346718704315 2216 13 -2.5016489483271656 2216 14 -2.7105852378315873 2216 15 -3.7336633292884436 2216 16 -2.826691079013113 2216 17 -3.026757716792414 2216 18 -2.769337254027171 2216 19 -2.0106729638738727 2216 20 -2.9317581506660346 2216 21 -5.8263853062957836 2216 22 -4.143874395669169 2216 23 -4.089623315589996 2216 24 -4.193739347977026 2216 25 -3.8742551424664704 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33851.0 3 2.0 4 230.0 5 1477.0 6 1915.0 7 1626.0 8 1249.0 9 1178.0 10 1095.0 11 1149.0 12 1095.0 13 1075.0 14 1063.0 15 1139.0 16 1254.0 17 1332.0 18 1577.0 19 1931.0 20 2523.0 21 3136.0 22 4145.0 23 4869.0 24 5852.0 25 6717.0 26 7495.0 27 8336.0 28 9532.0 29 11058.0 30 13244.0 31 16216.0 32 21205.0 33 28015.0 34 37733.0 35 53083.0 36 77369.0 37 123376.0 38 159698.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.39789018373873 18.44565420857138 12.255127187669688 25.901328420020203 2 14.210641017516581 19.91013729796735 42.3683938008028 23.510827883713272 3 19.71637234823602 24.06383554233162 28.73619537076394 27.48359673866842 4 11.605976069770243 16.69524330302665 38.58448334986144 33.11429727734166 5 12.47166828268106 38.027494889483144 36.09754139364258 13.403295434193218 6 31.61168700034275 38.006218986126164 18.032743857438852 12.349350156092228 7 28.048351913749304 30.990771460109517 22.078828853236647 18.882047772904535 8 24.317570998625236 37.85808944650177 21.202363111678082 16.621976443194907 9 26.135314479100085 15.662044199065624 20.120857302541587 38.081784019292705 10 14.182783993082095 29.479999073495005 35.03130814783931 21.305908785583583 11 34.43641640807126 22.621771417102803 24.594970126441574 18.346842048384357 12 23.286759710986228 24.880503921447538 32.068949546100164 19.763786821466063 13 29.05963377111389 22.373741194342767 25.411548205521683 23.155076829021656 14 20.070308374940947 22.783989328689337 27.695231566823836 29.45047072954588 15 22.66772265243865 32.17792433954574 22.873053219212768 22.281299788802848 16 21.448311425245404 28.427558182261702 28.888261032011435 21.235869360481452 17 21.15116510655248 29.29633523696598 28.26300082290041 21.28949883358113 18 20.921755884287265 27.956192434642187 30.408248963321817 20.713802717748734 19 23.412205195507006 27.176438800324238 27.364032886864553 22.047323117304206 20 23.227844388109567 27.750365130288984 28.34615277730603 20.67563770429541 21 22.17554617917734 27.32401150484695 27.28263604882921 23.2178062671465 22 22.486618508220335 28.913131076092714 27.433331840925828 21.166918574761127 23 21.468988484061565 28.49388075854818 28.20439980176344 21.832730955626815 24 23.01328030679639 27.175379713026803 28.73267400104365 21.078665979133152 25 21.971877084208185 27.989372082108538 28.406522490922114 21.63222834276116 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 176.0 1 176.0 2 227.5 3 279.0 4 279.0 5 279.0 6 425.0 7 571.0 8 571.0 9 571.0 10 855.0 11 1139.0 12 1139.0 13 1139.0 14 2017.5 15 2896.0 16 2896.0 17 2896.0 18 5146.0 19 7396.0 20 7396.0 21 7396.0 22 11699.0 23 16002.0 24 16002.0 25 16002.0 26 23256.5 27 30511.0 28 30511.0 29 30511.0 30 40596.0 31 50681.0 32 50681.0 33 50681.0 34 57899.5 35 65118.0 36 65118.0 37 65118.0 38 70789.5 39 76461.0 40 76461.0 41 76461.0 42 79648.5 43 82836.0 44 82836.0 45 82836.0 46 85000.5 47 87165.0 48 87165.0 49 87165.0 50 82775.0 51 78385.0 52 78385.0 53 78385.0 54 68598.5 55 58812.0 56 58812.0 57 58812.0 58 49722.0 59 40632.0 60 40632.0 61 40632.0 62 32852.5 63 25073.0 64 25073.0 65 25073.0 66 19307.5 67 13542.0 68 13542.0 69 13542.0 70 9871.5 71 6201.0 72 6201.0 73 6201.0 74 4351.0 75 2501.0 76 2501.0 77 2501.0 78 1756.5 79 1012.0 80 1012.0 81 1012.0 82 664.0 83 316.0 84 316.0 85 316.0 86 212.5 87 109.0 88 109.0 89 109.0 90 64.0 91 19.0 92 19.0 93 19.0 94 11.5 95 4.0 96 4.0 97 4.0 98 3.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22119659175104966 2 0.13166831316374414 3 0.1335206223758953 4 0.07069646826376882 5 0.02408001975796493 6 0.022536428747838972 7 0.29467152383304523 8 0.295134601136083 9 0.020684119535687825 10 0.03781797974808595 11 0.016979501111385527 12 0.018523092121511483 13 0.01589898740429736 14 0.018831810323536678 15 0.016053346505309953 16 0.020684119535687825 17 0.02022104223265004 18 0.015127191899234378 19 0.025469251667078294 20 0.019449246727587056 21 0.017442578414423312 22 0.018060014818473698 23 0.019912324030624845 24 0.01759693751543591 25 0.017288219313410718 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 647840.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.59811331188955 #Duplication Level Percentage of deduplicated Percentage of total 1 93.46438843144693 80.00375311581452 2 4.974106335914102 8.51548235533926 3 0.7646066790883427 1.963466674668946 4 0.2709270091563752 0.9276336331607496 5 0.1338501772945184 0.5728661321436346 6 0.0857789130004342 0.44055078688691296 7 0.05369200441302205 0.3217153994381834 8 0.043413468233653725 0.2972888778501127 9 0.032400009001259396 0.24960416776164387 >10 0.14812644359794225 2.364265918911505 >50 0.014987391402544042 0.8720547127843826 >100 0.01191746147095694 1.9981834462105121 >500 0.0010834055880166392 0.656117630571912 >1k 7.222703920110929E-4 0.8170171484577448 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1555 0.24002840207458634 No Hit GTCCTACAGTGTGCATTTCTCATTT 1375 0.21224376389231908 No Hit CTGTAGGACGTGGAATATGGCAAGA 1233 0.1903247715485305 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1123 0.17334527043714498 No Hit CTGTAGGACCTGGAATATGGCGAGA 845 0.13043344035564336 No Hit CTTTAGGACGTGAAATATGGCGAGG 836 0.12904420844653 No Hit GTATCAACGCAGAGTACTTTTTTTT 822 0.1268831810323537 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.543591010125957E-4 0.0 9 0.0 0.0 0.0 1.543591010125957E-4 0.0 10 0.0 0.0 0.0 1.543591010125957E-4 0.0 11 0.0 0.0 0.0 1.543591010125957E-4 0.0 12 0.0 0.0 0.0 1.543591010125957E-4 0.0 13 0.0 0.0 0.0 1.543591010125957E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGCCA 45 6.7086314E-4 14.7911415 8 TAGGCTA 40 0.005216279 14.273934 5 GAGGTAA 40 0.005332651 14.22655 9 GTATAAC 50 0.0014576125 13.348188 1 CTAAGAC 75 1.4260937E-5 12.708615 3 ACAACTG 115 5.167749E-9 12.412116 5 TAGGACC 255 0.0 12.318584 4 GTCCTAC 1050 0.0 12.167736 1 TCTAGGG 55 0.0029901264 12.130951 3 TCTAGAC 55 0.0029901264 12.130951 3 GACGTGG 370 0.0 12.087837 7 AGGACGT 585 0.0 11.874612 5 ATCTAGA 65 7.7727815E-4 11.734671 1 GTTTAGA 65 7.7727815E-4 11.734671 1 CTAACAC 65 7.793057E-4 11.731029 3 CTTAGAC 65 7.793057E-4 11.731029 3 GTAGGAC 700 0.0 11.71008 3 CTGTAGG 770 0.0 11.639421 1 GTCCTAA 290 0.0 11.507058 1 TGTAGGA 775 0.0 11.19178 2 >>END_MODULE