FastQCFastQC Report
Thu 26 May 2016
SRR1926961_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926961_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647840
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15440.23833045196344776No Hit
CTGTAGGACGTGGAATATGGCAAGA14960.23092121511484315No Hit
GTATCAACGCAGAGTACTTTTTTTT14240.21980735984193628No Hit
GTCCTACAGTGTGCATTTCTCATTT12910.19927759940726106No Hit
CTGTAGGACCTGGAATATGGCGAGA11370.17550629785132132No Hit
GTCCTAAAGTGTGTATTTCTCATTT11060.17072116571993085No Hit
CTTTAGGACGTGAAATATGGCGAGG10110.15605705112373425No Hit
GGTATCAACGCAGAGTACTTTTTTT8580.13244010866880712No Hit
GTACATGGGAAGCAGTGGTATCAAC7870.12148061249691283No Hit
TATCAACGCAGAGTACTTTTTTTTT7250.11191034823413187No Hit
CCCATGTACTCTGCGTTGATACCAC6520.1006421338602124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG651.8436367E-716.1256521
GTTGTAG551.9077458E-413.86006451
AGCACCG551.9253838E-413.845045
AACTGTC500.001507429513.2888127
TCAAGAC751.44510195E-512.692273
ATTTAGA1850.012.3616781
GACGTGG3900.012.1692427
TAGGACC5050.012.0630064
TGTAGGA10300.012.0257012
CCTATAC802.8017848E-511.8990033
GGCGAGG3200.011.86134819
TTTAGAA1850.011.8456912
TATACTG1459.276846E-1111.8160275
TCCTATA1052.6084308E-711.796642
CTGTAGG10050.011.5672921
CGTGGAA3800.011.4885539
TCAGGAC1001.8774826E-611.4230423
GGACGTG7300.011.34392456
GTAGGAC9850.011.3070733
AGGACGT7500.011.2952465