##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926961_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 647840 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.932264139293654 31.0 30.0 33.0 26.0 34.0 2 30.28722678439121 31.0 30.0 34.0 26.0 34.0 3 30.37081223758953 31.0 30.0 34.0 26.0 34.0 4 33.973828414423316 37.0 35.0 37.0 30.0 37.0 5 33.95135064213386 37.0 35.0 37.0 30.0 37.0 6 33.91147042479625 37.0 35.0 37.0 30.0 37.0 7 33.91374413435416 37.0 35.0 37.0 30.0 37.0 8 33.946898925660655 37.0 35.0 37.0 30.0 37.0 9 35.51811712768585 39.0 35.0 39.0 30.0 39.0 10 35.411312978513216 39.0 35.0 39.0 30.0 39.0 11 35.45859008397135 39.0 35.0 39.0 30.0 39.0 12 35.348113731785624 39.0 35.0 39.0 30.0 39.0 13 35.37043560138306 39.0 35.0 39.0 30.0 39.0 14 36.46724962953816 40.0 37.0 41.0 30.0 41.0 15 36.46418714497407 40.0 37.0 41.0 30.0 41.0 16 36.439691899234376 40.0 37.0 41.0 30.0 41.0 17 36.430482835267966 40.0 37.0 41.0 30.0 41.0 18 36.40453970116078 40.0 37.0 41.0 30.0 41.0 19 36.47313379846876 40.0 37.0 41.0 30.0 41.0 20 36.435144480118545 40.0 37.0 41.0 30.0 41.0 21 36.38821005186466 40.0 37.0 41.0 30.0 41.0 22 36.311438009385036 40.0 37.0 41.0 30.0 41.0 23 36.23963169918498 40.0 37.0 41.0 30.0 41.0 24 36.18151704124475 40.0 37.0 41.0 29.0 41.0 25 36.06171276858483 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2988830899280117 1101 2 -0.11200967462582412 1101 3 0.17194443368656565 1101 4 -0.1413713309212099 1101 5 -0.026117464092941134 1101 6 -0.22693335861491448 1101 7 -0.2091223299094196 1101 8 0.08456321876047213 1101 9 -0.010220296826652486 1101 10 -0.04026164474542071 1101 11 -0.09880749410593381 1101 12 0.05784966617649445 1101 13 -0.23614337954661124 1101 14 0.021554330754554485 1101 15 -0.27274020713227287 1101 16 -0.05651129850994607 1101 17 -0.1763320435834146 1101 18 -0.06491464857669627 1101 19 0.09128874709560364 1101 20 -0.08190135181495606 1101 21 -0.17965360653690965 1101 22 -0.11003919411809449 1101 23 0.028929572382409674 1101 24 0.12653896608507154 1101 25 -0.0784694253398115 1102 1 -0.7815272848703643 1102 2 -0.6450534839195683 1102 3 -0.29921442097157325 1102 4 -0.518000982079613 1102 5 -0.58555059514768 1102 6 -0.5540142811950943 1102 7 -0.638273956891446 1102 8 -0.4881379068028764 1102 9 -0.6714189349780426 1102 10 -0.6472904573122165 1102 11 -0.6062309062980802 1102 12 -0.4368384987465177 1102 13 -0.6195596266119168 1102 14 -0.7178395236717279 1102 15 -0.5737143581067983 1102 16 -0.8764427315676642 1102 17 -0.6693518684670252 1102 18 -0.8116526501706289 1102 19 -0.7622650760129019 1102 20 -0.5948301782935772 1102 21 -0.7397284068375782 1102 22 -0.6840711093444156 1102 23 -0.5981290265786257 1102 24 -0.5672446069665469 1102 25 -0.750323008280688 1103 1 -0.6176159030620667 1103 2 -0.6170356753754973 1103 3 -0.3335481763066106 1103 4 -0.42852398622634524 1103 5 -0.42351926883134894 1103 6 -0.6095993780731845 1103 7 -0.46632685402880014 1103 8 -0.4873570773042033 1103 9 -0.6128870460529328 1103 10 -0.49702897148356584 1103 11 -0.3521851590030991 1103 12 -0.40298667007849076 1103 13 -0.349301649642598 1103 14 -0.42935605483938133 1103 15 -0.38763001769113004 1103 16 -0.29385728705258884 1103 17 -0.18988140299084222 1103 18 -0.4700619456154982 1103 19 -0.19163899370849435 1103 20 -0.2646031962483235 1103 21 -0.22708189300844595 1103 22 -0.35776668547421053 1103 23 -0.6220445020561414 1103 24 -0.5990560989140405 1103 25 -0.26799238925941893 1104 1 -0.41886327539629065 1104 2 -0.37479404245171466 1104 3 -0.09333486066043406 1104 4 -0.1098353318586689 1104 5 -0.06969665031225247 1104 6 -0.1363745568614334 1104 7 -0.06323730745827305 1104 8 0.07544533827618949 1104 9 0.03997864746362012 1104 10 -0.034396117236781265 1104 11 0.050068883456766855 1104 12 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-0.7004035978990117 2216 24 -0.8594905094926446 2216 25 -0.6883943557140171 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14167.0 3 17.0 4 266.0 5 1499.0 6 2010.0 7 1481.0 8 1127.0 9 1018.0 10 902.0 11 851.0 12 759.0 13 703.0 14 753.0 15 733.0 16 732.0 17 747.0 18 933.0 19 1087.0 20 1455.0 21 1843.0 22 2310.0 23 2900.0 24 3401.0 25 4126.0 26 5004.0 27 5404.0 28 6378.0 29 7804.0 30 10021.0 31 13138.0 32 16948.0 33 22596.0 34 30766.0 35 43450.0 36 64077.0 37 112204.0 38 264230.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.00067071257803 19.191034087344086 10.358182152554658 28.450113047523228 2 14.156327217489265 20.586849055116772 40.366270705297495 24.89055302209647 3 18.912017488741466 24.1683661890507 28.916787987605968 28.002828334601865 4 11.960978019264015 17.008983699678932 37.86860953321808 33.161428747838976 5 12.69907672548599 38.68460010219156 35.47297558053914 13.143347591783305 6 32.106307475693114 37.83881325714569 17.46672470062686 12.588154566534335 7 28.832950178556814 31.24767443835274 21.33603420138862 18.583341181701822 8 24.945775423028888 38.72028454587978 19.3777411392024 16.95619889188893 9 26.284727153716474 15.643351591011543 19.756423635768584 38.3154976195034 10 14.489433630231085 29.840385298158413 34.20879578888872 21.461385282721785 11 34.51198747763588 22.801601108982542 24.23892522549363 18.447486187887947 12 23.699788681412144 24.83232665006784 31.577078802229575 19.89080586629044 13 29.389300604633 22.209341633852038 25.299190683990112 23.10216707752485 14 20.253766362064706 22.89608545319832 27.40244504816004 29.44770313657693 15 22.607782906009295 32.186376055446644 22.7493323865829 22.456508651961162 16 21.564541424319884 28.61961125723953 28.457069116684576 21.35877820175601 17 21.13673304632787 29.47059377407041 27.992911819140247 21.399761360461472 18 20.92187196102705 27.97445024497016 30.34758904287209 20.75608875113069 19 23.23524826384333 27.150264186009178 27.59821498860056 22.016272561546927 20 23.274789529416488 27.78366062911178 28.327627139051053 20.61392270242068 21 22.154228056958665 27.07805881117931 27.3721455829865 23.395567548875523 22 22.583697492741255 28.849760511485105 27.257235208130414 21.309306787643226 23 21.405130896517658 28.61786860953322 28.025901457149914 21.95109903679921 24 22.785908227198423 27.17372680557978 28.854545651002795 21.185819316219 25 22.022605812305688 28.07727100114542 28.27980327200763 21.620319914541263 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 180.0 1 180.0 2 179.5 3 179.0 4 179.0 5 179.0 6 310.5 7 442.0 8 442.0 9 442.0 10 736.0 11 1030.0 12 1030.0 13 1030.0 14 1807.0 15 2584.0 16 2584.0 17 2584.0 18 4580.0 19 6576.0 20 6576.0 21 6576.0 22 10818.0 23 15060.0 24 15060.0 25 15060.0 26 22182.0 27 29304.0 28 29304.0 29 29304.0 30 39127.0 31 48950.0 32 48950.0 33 48950.0 34 56446.0 35 63942.0 36 63942.0 37 63942.0 38 69739.5 39 75537.0 40 75537.0 41 75537.0 42 79245.0 43 82953.0 44 82953.0 45 82953.0 46 85917.5 47 88882.0 48 88882.0 49 88882.0 50 84656.0 51 80430.0 52 80430.0 53 80430.0 54 69982.5 55 59535.0 56 59535.0 57 59535.0 58 50694.5 59 41854.0 60 41854.0 61 41854.0 62 33841.0 63 25828.0 64 25828.0 65 25828.0 66 19953.0 67 14078.0 68 14078.0 69 14078.0 70 10240.5 71 6403.0 72 6403.0 73 6403.0 74 4494.0 75 2585.0 76 2585.0 77 2585.0 78 1800.5 79 1016.0 80 1016.0 81 1016.0 82 680.0 83 344.0 84 344.0 85 344.0 86 230.5 87 117.0 88 117.0 89 117.0 90 69.0 91 21.0 92 21.0 93 21.0 94 15.0 95 9.0 96 9.0 97 9.0 98 5.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11839343047666091 2 0.11160163003210669 3 0.016825142010372933 4 0.0 5 0.005711286737466041 6 0.34591874536922695 7 0.022690787848851566 8 0.010959496171894295 9 0.011731291676957273 10 0.004630773030377871 11 0.006020004939491233 12 4.6307730303778714E-4 13 0.0013892319091133613 14 0.0 15 0.001543591010125957 16 0.0012348728081007657 17 1.543591010125957E-4 18 9.261546060755743E-4 19 1.543591010125957E-4 20 9.261546060755743E-4 21 0.01111385527290689 22 1.543591010125957E-4 23 0.0 24 1.543591010125957E-4 25 0.00648308224252902 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 647840.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.08104139340156 #Duplication Level Percentage of deduplicated Percentage of total 1 91.94416874040384 76.38817289003389 2 6.156235508051475 10.229329141439061 3 0.9559563886624535 2.382655568902559 4 0.33664248213379255 1.1187443197174023 5 0.1608035676767787 0.6679863931180549 6 0.09948717915355604 0.49592990696216077 7 0.06637280961833536 0.3860025500308096 8 0.04321547441278054 0.2872309294818965 9 0.03088594298924016 0.2309432677166972 >10 0.1727427583615882 2.639170059650985 >50 0.017674964081770838 0.9979276325809826 >100 0.013581593708646327 2.231705292635956 >500 9.302461440798599E-4 0.5516661717559816 >1k 0.0013023446017118038 1.3925358759735609 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1544 0.23833045196344776 No Hit CTGTAGGACGTGGAATATGGCAAGA 1496 0.23092121511484315 No Hit GTATCAACGCAGAGTACTTTTTTTT 1424 0.21980735984193628 No Hit GTCCTACAGTGTGCATTTCTCATTT 1291 0.19927759940726106 No Hit CTGTAGGACCTGGAATATGGCGAGA 1137 0.17550629785132132 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1106 0.17072116571993085 No Hit CTTTAGGACGTGAAATATGGCGAGG 1011 0.15605705112373425 No Hit GGTATCAACGCAGAGTACTTTTTTT 858 0.13244010866880712 No Hit GTACATGGGAAGCAGTGGTATCAAC 787 0.12148061249691283 No Hit TATCAACGCAGAGTACTTTTTTTTT 725 0.11191034823413187 No Hit CCCATGTACTCTGCGTTGATACCAC 652 0.1006421338602124 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTTAG 65 1.8436367E-7 16.125652 1 GTTGTAG 55 1.9077458E-4 13.8600645 1 AGCACCG 55 1.9253838E-4 13.84504 5 AACTGTC 50 0.0015074295 13.288812 7 TCAAGAC 75 1.44510195E-5 12.69227 3 ATTTAGA 185 0.0 12.361678 1 GACGTGG 390 0.0 12.169242 7 TAGGACC 505 0.0 12.063006 4 TGTAGGA 1030 0.0 12.025701 2 CCTATAC 80 2.8017848E-5 11.899003 3 GGCGAGG 320 0.0 11.861348 19 TTTAGAA 185 0.0 11.845691 2 TATACTG 145 9.276846E-11 11.816027 5 TCCTATA 105 2.6084308E-7 11.79664 2 CTGTAGG 1005 0.0 11.567292 1 CGTGGAA 380 0.0 11.488553 9 TCAGGAC 100 1.8774826E-6 11.423042 3 GGACGTG 730 0.0 11.3439245 6 GTAGGAC 985 0.0 11.307073 3 AGGACGT 750 0.0 11.295246 5 >>END_MODULE