FastQCFastQC Report
Thu 26 May 2016
SRR1926960_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926960_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639118
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17820.2788217512259082No Hit
CTGTAGGACGTGGAATATGGCAAGA15190.23767129074756146No Hit
GTCCTACAGTGTGCATTTCTCATTT13920.21780015583976667No Hit
GTCCTAAAGTGTGTATTTCTCATTT13820.21623549954781435No Hit
CTTTAGGACGTGAAATATGGCGAGG11740.18369064867520551No Hit
CTGTAGGACCTGGAATATGGCGAGA9290.14535656952237302No Hit
GTATCAACGCAGAGTACTTTTTTTT8010.12532896898538298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3450.012.974484
TCTACAC1103.59978E-812.1374473
TTGGGCC550.003008067412.1212264
TAAACTA550.003022270312.1136075
GGACGAG550.003022270312.1136076
GGTATCA2950.011.9686551
GTATAAG802.7321614E-511.928221
CTAGGAC1552.3646862E-1111.6899843
CTACACT1251.7564162E-811.4285854
AGGACAC752.0343975E-411.4214025
GTCCTAA4750.011.2501951
GTCTTAA600.005677013711.1330051
GTGTAGC1407.743438E-910.90581
CTACCCT700.001458512210.8843664
GTCCTAC10750.010.8297151
GTAGGAC7950.010.7961123
CAGTACT803.677969E-410.7142984
GGACCTG5550.010.6325366
TGTAGGA8350.010.62242
CCTACAG10600.010.6161773
Produced by FastQC (version 0.11.5)