##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926960_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 639118 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.439465012720657 31.0 28.0 31.0 22.0 34.0 2 28.476849345504274 31.0 28.0 31.0 22.0 34.0 3 28.04316573778238 31.0 28.0 31.0 16.0 34.0 4 31.90027037260725 35.0 32.0 37.0 19.0 37.0 5 31.74945941125113 35.0 32.0 37.0 19.0 37.0 6 31.374597492168895 35.0 32.0 37.0 17.0 37.0 7 31.201321195772923 35.0 32.0 37.0 17.0 37.0 8 31.341312558870193 35.0 32.0 37.0 17.0 37.0 9 33.53500136125098 37.0 34.0 39.0 19.0 39.0 10 33.709775033718344 38.0 34.0 39.0 22.0 39.0 11 33.84828936127601 38.0 34.0 39.0 23.0 39.0 12 33.75169999906121 38.0 34.0 39.0 22.0 39.0 13 33.848680525348996 38.0 34.0 39.0 23.0 39.0 14 34.897863931230226 39.0 35.0 41.0 24.0 41.0 15 34.5085743164799 39.0 34.0 41.0 18.0 41.0 16 34.597697138869506 39.0 34.0 41.0 19.0 41.0 17 34.82215177791895 39.0 35.0 41.0 23.0 41.0 18 34.86420973904662 39.0 35.0 41.0 23.0 41.0 19 34.25247606858201 39.0 34.0 41.0 18.0 41.0 20 34.54838386651604 39.0 34.0 41.0 18.0 41.0 21 34.526747799310925 39.0 34.0 41.0 18.0 41.0 22 34.55784847242606 39.0 34.0 41.0 18.0 41.0 23 34.54956987598534 39.0 35.0 41.0 18.0 41.0 24 33.290129522247845 39.0 33.0 41.0 2.0 41.0 25 33.85365456770111 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.44843984862677644 1101 2 -0.5921168781988797 1101 3 -1.4480918197544348 1101 4 -0.8178275664604264 1101 5 -0.8230358927681287 1101 6 -2.922709647458131 1101 7 -1.111406965216279 1101 8 -0.7565323811981237 1101 9 -0.7136315473598671 1101 10 -9.736728645256097 1101 11 -4.7783122210873366 1101 12 -2.0012135345680413 1101 13 -1.226180378255279 1101 14 -1.0721179264035214 1101 15 -2.228531565400978 1101 16 -0.7234208861064033 1101 17 -0.7882528696371907 1101 18 -0.5476814651524151 1101 19 -1.2676407218522883 1101 20 -0.38567857347143075 1101 21 -0.5810262452564174 1101 22 -0.36443537105845536 1101 23 -0.5233709898866934 1101 24 -0.6096762139951366 1101 25 -0.606732789199782 1102 1 -0.4494309379157393 1102 2 -0.47290429409267887 1102 3 -0.8290792720184825 1102 4 -0.7607626455657197 1102 5 -0.743230473601006 1102 6 -1.1728960449849524 1102 7 -0.5596884709445931 1102 8 0.15942506561038527 1102 9 0.054921825980734695 1102 10 -0.050853940764369554 1102 11 -0.3421101843533876 1102 12 -0.26813479297866394 1102 13 -0.18592305874263815 1102 14 -0.5021824835957318 1102 15 0.0933496857355891 1102 16 0.00585261816710414 1102 17 -0.2825518330850869 1102 18 -0.3871204540594846 1102 19 -0.07857907428000033 1102 20 -0.06228341071456356 1102 21 -0.25212553603655863 1102 22 -0.37098656218411463 1102 23 -0.7507077783936964 1102 24 -4.085444535508138 1102 25 -2.4475129310437502 1103 1 -0.1641491450745498 1103 2 -0.2546002199570694 1103 3 -0.1776975364034854 1103 4 -0.12404281392148064 1103 5 -0.2367048418053983 1103 6 0.49347756820291266 1103 7 -0.09474412803048793 1103 8 0.22745962284580656 1103 9 0.4135615963375727 1103 10 0.3323054064878477 1103 11 -0.05909205657283678 1103 12 0.037734583625052665 1103 13 0.0020949767376023942 1103 14 0.025191480333305094 1103 15 0.24607208332840003 1103 16 0.48133073376401114 1103 17 0.1838600847307177 1103 18 -0.16377447558392078 1103 19 -1.634956102579281 1103 20 -0.38196091343742467 1103 21 -0.30081164114814385 1103 22 -0.09981517897169567 1103 23 -0.060363775144722354 1103 24 1.324862639917832 1103 25 0.6563387608209013 1104 1 -0.3597892037144206 1104 2 -0.2795714036095269 1104 3 -1.3693077827133138 1104 4 -0.5790501439050288 1104 5 -0.530923666993683 1104 6 -1.8246884811038164 1104 7 -0.6653480962933358 1104 8 0.03559086871919348 1104 9 0.10912779560121777 1104 10 0.18672521870598047 1104 11 1.521158104722531E-4 1104 12 0.06846456046007177 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0.6706019106618868 2215 2 0.05411605289035393 2215 3 0.5546511145104098 2215 4 0.7086451232823521 2215 5 1.061264678841905 2215 6 1.4595267768035391 2215 7 0.9954205990898473 2215 8 -0.10643314334483733 2215 9 0.04189401631114009 2215 10 -0.10454382467813872 2215 11 0.06645595928738857 2215 12 -0.12186294707995415 2215 13 0.1250620796678703 2215 14 -0.045220498648674834 2215 15 0.17980044476097845 2215 16 0.3112806064309126 2215 17 -0.08404915417196435 2215 18 -0.8642938817926122 2215 19 0.30163523877013176 2215 20 -3.2420496436524395 2215 21 -1.7658071280763608 2215 22 -0.9042178416840514 2215 23 -0.6679803896961545 2215 24 -0.8433218157734785 2215 25 -0.5348885529342979 2216 1 -0.6559602919860765 2216 2 -1.2902295007621483 2216 3 -0.26378573131510663 2216 4 -0.6487057589186662 2216 5 -0.3285604441768797 2216 6 -1.8708959048700606 2216 7 -10.5851193242443 2216 8 -11.850593149325864 2216 9 -6.381168267896243 2216 10 -3.881682720363642 2216 11 -2.8268201465223655 2216 12 -2.3353549134774703 2216 13 -2.191475111425838 2216 14 -2.1901991572681467 2216 15 -3.0924601783529617 2216 16 -2.21813855459456 2216 17 -2.5216579000600063 2216 18 -2.030160246689441 2216 19 -1.7735489495777443 2216 20 -2.5291812669566838 2216 21 -5.082954315635828 2216 22 -3.656137886269864 2216 23 -3.3616262315252605 2216 24 -3.7935501821383717 2216 25 -3.068186883684916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32892.0 3 2.0 4 238.0 5 1483.0 6 1812.0 7 1445.0 8 1200.0 9 1117.0 10 1055.0 11 1089.0 12 1026.0 13 1001.0 14 1042.0 15 1135.0 16 1117.0 17 1276.0 18 1497.0 19 1829.0 20 2376.0 21 3187.0 22 3984.0 23 4526.0 24 5641.0 25 6258.0 26 7413.0 27 8124.0 28 9058.0 29 10847.0 30 12956.0 31 16084.0 32 20591.0 33 27040.0 34 37470.0 35 52426.0 36 76858.0 37 123521.0 38 158502.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73530006727228 19.019392918691498 12.337170518749206 25.90813649528702 2 13.838479266643791 20.315195269720856 43.139042820369404 22.70728264326595 3 19.213926591159417 24.640860087017693 29.2689041003602 26.87630922146269 4 11.208748535960567 17.25687550497617 39.50651693275127 32.027859026312 5 12.043245002848362 38.3121740817949 36.82257529375677 12.822005621599967 6 30.52980661033634 38.98811992444078 18.465371559496628 12.016701905726256 7 27.28970525228778 31.231392883430193 22.882692576299966 18.59620928798206 8 23.503212502236355 38.76173497091956 21.667833232370473 16.067219294473617 9 26.075015141058326 15.621346779532105 20.639101333195615 37.66453674621395 10 13.738478225822861 29.642869999796524 36.076437746811315 20.5422140275693 11 33.822786077904524 22.62864761749761 25.441579435786128 18.106986868811745 12 22.96887030441124 25.077777290908976 32.86176612352975 19.09158628115004 13 28.932674822931435 22.730658604295968 25.845281353576894 22.491385219195703 14 19.435593920576892 23.299864790425158 28.38634258100055 28.87819870799739 15 21.988566921691394 33.12994993920407 23.235939864449325 21.645543274655214 16 20.63406610776119 28.867723443512933 29.815473554235062 20.682736894490812 17 20.56513832680745 29.801531782771047 28.883995780868048 20.749334109553455 18 20.24741009671059 28.22994585458984 31.385872116678666 20.13677193202091 19 23.124981415315656 27.287324697519587 27.820216067496446 21.767477819668308 20 22.801663834142403 28.15982948516915 29.19033624097426 19.84817043971418 21 21.682855493011964 27.60691745654681 27.696116403060305 23.014110647380914 22 21.986300619555028 29.666028697651196 27.986078399767138 20.361592283026635 23 20.840440815151222 29.204160263412405 28.816678612452623 21.138720308983753 24 22.58917644996823 27.56434131333586 29.502327022569148 20.344155214126765 25 21.52104547424889 28.488578622611776 29.056165603321332 20.934210299818005 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 176.0 1 176.0 2 220.0 3 264.0 4 264.0 5 264.0 6 419.0 7 574.0 8 574.0 9 574.0 10 951.5 11 1329.0 12 1329.0 13 1329.0 14 2324.0 15 3319.0 16 3319.0 17 3319.0 18 5806.0 19 8293.0 20 8293.0 21 8293.0 22 13081.5 23 17870.0 24 17870.0 25 17870.0 26 25976.5 27 34083.0 28 34083.0 29 34083.0 30 44581.5 31 55080.0 32 55080.0 33 55080.0 34 62611.5 35 70143.0 36 70143.0 37 70143.0 38 75012.0 39 79881.0 40 79881.0 41 79881.0 42 82048.0 43 84215.0 44 84215.0 45 84215.0 46 84514.5 47 84814.0 48 84814.0 49 84814.0 50 79285.5 51 73757.0 52 73757.0 53 73757.0 54 62834.5 55 51912.0 56 51912.0 57 51912.0 58 43386.5 59 34861.0 60 34861.0 61 34861.0 62 27753.5 63 20646.0 64 20646.0 65 20646.0 66 15620.5 67 10595.0 68 10595.0 69 10595.0 70 7623.0 71 4651.0 72 4651.0 73 4651.0 74 3203.5 75 1756.0 76 1756.0 77 1756.0 78 1193.0 79 630.0 80 630.0 81 630.0 82 409.5 83 189.0 84 189.0 85 189.0 86 123.5 87 58.0 88 58.0 89 58.0 90 36.5 91 15.0 92 15.0 93 15.0 94 9.0 95 3.0 96 3.0 97 3.0 98 3.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22077300279447612 2 0.13252638792836377 3 0.13409104422031612 4 0.07416470823854124 5 0.02409570689606614 6 0.02331337875008997 7 0.30182219871760774 8 0.3004140080548506 9 0.019871134907794803 10 0.03426597279375639 11 0.01642889106549964 12 0.017837081728256755 13 0.015646562919523467 14 0.017211219211475812 15 0.014864234773547294 16 0.020496997424575746 17 0.0194017380202091 18 0.015333631661133 19 0.023626310008480436 20 0.017524150469866284 21 0.015177166031937764 22 0.017367684840671048 23 0.01877587550342816 24 0.01627242543630441 25 0.015177166031937764 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 639118.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.11170806020189 #Duplication Level Percentage of deduplicated Percentage of total 1 94.1471643666076 81.07173132633156 2 4.501862001212197 7.753260527514019 3 0.6469902264280109 1.671403004879184 4 0.21800101512093667 0.7508975908368702 5 0.11850134815811798 0.51021767486661 6 0.07348945619877872 0.37969815586153366 7 0.052165326338325296 0.31444317467577204 8 0.037090919439161 0.25551699411435996 9 0.026845704302884996 0.2080556506440485 >10 0.1484050131950281 2.4198305275810705 >50 0.0161984335550803 1.005247210231199 >100 0.01146616349335339 1.9927652837235976 >500 9.100129752934982E-4 0.530829415221246 >1k 9.100129752934982E-4 1.1361034635189882 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1782 0.2788217512259082 No Hit CTGTAGGACGTGGAATATGGCAAGA 1519 0.23767129074756146 No Hit GTCCTACAGTGTGCATTTCTCATTT 1392 0.21780015583976667 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1382 0.21623549954781435 No Hit CTTTAGGACGTGAAATATGGCGAGG 1174 0.18369064867520551 No Hit CTGTAGGACCTGGAATATGGCGAGA 929 0.14535656952237302 No Hit GTATCAACGCAGAGTACTTTTTTTT 801 0.12532896898538298 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.5646562919523468E-4 0.0 9 0.0 0.0 0.0 1.5646562919523468E-4 0.0 10 0.0 0.0 0.0 1.5646562919523468E-4 0.0 11 0.0 0.0 0.0 1.5646562919523468E-4 0.0 12 0.0 0.0 0.0 1.5646562919523468E-4 0.0 13 0.0 0.0 0.0 1.5646562919523468E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 345 0.0 12.97448 4 TCTACAC 110 3.59978E-8 12.137447 3 TTGGGCC 55 0.0030080674 12.121226 4 TAAACTA 55 0.0030222703 12.113607 5 GGACGAG 55 0.0030222703 12.113607 6 GGTATCA 295 0.0 11.968655 1 GTATAAG 80 2.7321614E-5 11.92822 1 CTAGGAC 155 2.3646862E-11 11.689984 3 CTACACT 125 1.7564162E-8 11.428585 4 AGGACAC 75 2.0343975E-4 11.421402 5 GTCCTAA 475 0.0 11.250195 1 GTCTTAA 60 0.0056770137 11.133005 1 GTGTAGC 140 7.743438E-9 10.9058 1 CTACCCT 70 0.0014585122 10.884366 4 GTCCTAC 1075 0.0 10.829715 1 GTAGGAC 795 0.0 10.796112 3 CAGTACT 80 3.677969E-4 10.714298 4 GGACCTG 555 0.0 10.632536 6 TGTAGGA 835 0.0 10.6224 2 CCTACAG 1060 0.0 10.616177 3 >>END_MODULE