FastQCFastQC Report
Thu 26 May 2016
SRR1926960_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926960_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639118
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18160.2841415826185462No Hit
CTGTAGGACGTGGAATATGGCAAGA18030.28210752943900813No Hit
GTCCTAAAGTGTGTATTTCTCATTT14360.22468464352435702No Hit
GTCCTACAGTGTGCATTTCTCATTT14190.22202472782803803No Hit
GTATCAACGCAGAGTACTTTTTTTT14160.22155533094045232No Hit
CTTTAGGACGTGAAATATGGCGAGG13590.21263679007632394No Hit
CTGTAGGACCTGGAATATGGCGAGA10660.16679236072212017No Hit
GGTATCAACGCAGAGTACTTTTTTT8630.13502983799548754No Hit
TATCAACGCAGAGTACTTTTTTTTT7630.11938327507596405No Hit
CTGAAGGACCTGGAATATGGCGAGA6900.10796128414471193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAC807.4342097E-915.4764913
TAGGACC3600.014.5494054
CTATACC602.5051593E-514.284874
GGCGAGG3400.013.11504719
TGTAGGA9750.013.0049842
TAGGACT1102.6357156E-912.9862454
CTCCTAG603.9657566E-412.7116381
GTCTAGA751.4225399E-512.71163751
CTATTCT751.4387293E-512.6976614
GTAGGAC9300.012.4938433
GACGTGG3800.012.4864597
ATACTTA550.003009431312.1204966
CTGTAGG9400.012.0692951
GTCCTAA4150.011.9458771
AGGACCT6100.011.8650295
GGACCTG5550.011.8397136
GTGTAGG1302.4592737E-911.7338211
CTAAATT5100.011.719830519
GGACGTG7800.011.5988266
AGGACGT8050.011.5935175