##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926960_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 639118 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.016191063309122 31.0 30.0 33.0 26.0 34.0 2 30.29606113425064 31.0 30.0 34.0 26.0 34.0 3 30.331688358018393 31.0 30.0 34.0 26.0 34.0 4 33.916098435656636 35.0 35.0 37.0 30.0 37.0 5 33.96575280308175 37.0 35.0 37.0 30.0 37.0 6 33.93231922743531 37.0 35.0 37.0 30.0 37.0 7 33.94422782647336 37.0 35.0 37.0 30.0 37.0 8 33.98995803591825 37.0 35.0 37.0 30.0 37.0 9 35.540299287455525 39.0 35.0 39.0 30.0 39.0 10 35.426428296496105 39.0 35.0 39.0 30.0 39.0 11 35.476910367099656 39.0 35.0 39.0 30.0 39.0 12 35.37339740079297 39.0 35.0 39.0 30.0 39.0 13 35.38372726163244 39.0 35.0 39.0 30.0 39.0 14 36.50186037633113 40.0 37.0 41.0 30.0 41.0 15 36.49986856887148 40.0 37.0 41.0 30.0 41.0 16 36.48901611283049 40.0 37.0 41.0 30.0 41.0 17 36.46608607487193 40.0 37.0 41.0 30.0 41.0 18 36.444348305007836 40.0 37.0 41.0 30.0 41.0 19 36.51175682737773 40.0 37.0 41.0 30.0 41.0 20 36.48013981768625 40.0 37.0 41.0 30.0 41.0 21 36.42972033333437 40.0 37.0 41.0 30.0 41.0 22 36.341853929947206 40.0 37.0 41.0 30.0 41.0 23 36.28036293767348 40.0 37.0 41.0 30.0 41.0 24 36.20893481328957 40.0 37.0 41.0 30.0 41.0 25 36.08589024249043 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.23106889878392067 1101 2 -0.2770535288652063 1101 3 -0.06644556913286692 1101 4 -0.06415147788845843 1101 5 -0.14235890309861787 1101 6 -0.19657265953276237 1101 7 0.03655908693741594 1101 8 -0.03243315095210164 1101 9 -0.04394480326485706 1101 10 0.1516917234288826 1101 11 -0.018249409804980132 1101 12 -0.0910431206450184 1101 13 -0.060890308500169965 1101 14 -0.16852008818911202 1101 15 -0.10010037578367559 1101 16 -0.08082800796740486 1101 17 -0.11465814587481304 1101 18 -0.25406225280297434 1101 19 -0.3229525788445571 1101 20 -0.3460826430417612 1101 21 -0.46620375476636156 1101 22 -0.28360075716126687 1101 23 -0.37276079341226875 1101 24 -0.24751609672347996 1101 25 -0.4663183304623928 1102 1 -0.8035957218426582 1102 2 -0.8517716594344016 1102 3 -0.6977739924825634 1102 4 -0.8158517870355766 1102 5 -0.7638991831495403 1102 6 -0.6945722958302696 1102 7 -0.5051666813840967 1102 8 -0.5272640292936188 1102 9 -0.4755960125756431 1102 10 -0.4205032211064861 1102 11 -0.42869676386935396 1102 12 -0.3259358284100671 1102 13 -0.3405775243575988 1102 14 -0.5239392553104025 1102 15 -0.5941720251744727 1102 16 -0.4967763622136374 1102 17 -0.5938998261803192 1102 18 -0.7018436676056652 1102 19 -0.43959196782437004 1102 20 -0.4587758599902969 1102 21 -0.5475549095217715 1102 22 -0.38262785299687607 1102 23 -0.4468515371838606 1102 24 -0.40889998470311184 1102 25 -0.6698644297531757 1103 1 -0.30892837215371216 1103 2 -0.34700665601928904 1103 3 -0.2362424534672236 1103 4 -0.3180069992339867 1103 5 -0.2888664165401025 1103 6 -0.1641178091122839 1103 7 -0.194751514597435 1103 8 -0.10863828858364855 1103 9 -0.18658990229523198 1103 10 -0.05620313434419444 1103 11 0.045869886183254494 1103 12 -0.4182661342932903 1103 13 -0.2938874134126266 1103 14 -0.24449545696811725 1103 15 -0.14716163215975797 1103 16 -0.3412457277413168 1103 17 -0.19024750298156334 1103 18 -0.286386134977505 1103 19 -0.26583663748156994 1103 20 -0.2415286243845287 1103 21 -0.10929920074769939 1103 22 -0.05348495365959138 1103 23 -0.002589385848274617 1103 24 -0.057660391562869506 1103 25 -0.20424167957318673 1104 1 -0.46678712169208936 1104 2 -0.5064824782643917 1104 3 -0.17808155193520392 1104 4 -0.4530667022297692 1104 5 -0.24934269760859706 1104 6 -0.6027406674701439 1104 7 -0.549611125828541 1104 8 -0.4500387071201146 1104 9 -0.401810529204667 1104 10 -0.46919902731524843 1104 11 -0.3150193359432194 1104 12 -0.20931012538540017 1104 13 -0.18584345589295737 1104 14 -0.1719375724797061 1104 15 -0.24173856341458588 1104 16 -0.1673068967338054 1104 17 -0.2721049543854477 1104 18 -0.2960091164343268 1104 19 -0.31897286326744023 1104 20 -0.14426365946773245 1104 21 -0.10360867153408293 1104 22 -0.17576810365007844 1104 23 -0.3321588752114053 1104 24 -0.39741687943762116 1104 25 -0.4326450860571427 1105 1 -0.016986547643558225 1105 2 -0.20256904350516436 1105 3 -0.06324095956823683 1105 4 -0.14752271403024508 1105 5 -0.1626219631672825 1105 6 -0.3041747237780257 1105 7 -0.4316255143177514 1105 8 -0.38152457689949415 1105 9 -0.4923408584571831 1105 10 -0.04975805899442065 1105 11 -0.09741234085831252 1105 12 -0.04726975028356151 1105 13 -0.20683616579609776 1105 14 -0.09858503319359357 1105 15 -0.30645055399975973 1105 16 -0.32625124384679083 1105 17 -0.15144719487877012 1105 18 -0.2995215692404045 1105 19 -0.19737255654020913 1105 20 -0.14596237451494432 1105 21 0.05198084648881007 1105 22 -0.23484717643921726 1105 23 -0.13650311262680503 1105 24 -0.1226893927371222 1105 25 -0.026921641126932627 1106 1 0.12145769696076059 1106 2 0.1892806909929483 1106 3 0.396697161363587 1106 4 0.42122086253706925 1106 5 0.2397332954829423 1106 6 0.45096723408425987 1106 7 0.3191388741714576 1106 8 0.18924238096278856 1106 9 -0.2021451578747886 1106 10 -0.2234412552945173 1106 11 -0.21211022540781954 1106 12 -0.077191170290412 1106 13 0.08904320213812866 1106 14 -0.022309981806131418 1106 15 -0.12587608500353298 1106 16 -0.04370478101705544 1106 17 -0.09814662105211625 1106 18 -0.07112785563985824 1106 19 -0.2043355575679584 1106 20 0.07517178958235604 1106 21 -0.0508668753337318 1106 22 0.27103133503731414 1106 23 0.05093158247425578 1106 24 0.09443869051056453 1106 25 0.15930223691349 1107 1 0.41804802449402345 1107 2 0.40811491992505466 1107 3 0.4412734578494302 1107 4 0.3825949669382993 1107 5 0.45953029309467297 1107 6 0.5858540048109759 1107 7 0.43728754356757094 1107 8 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-0.31421779951637774 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13926.0 3 11.0 4 190.0 5 1376.0 6 1986.0 7 1496.0 8 1099.0 9 997.0 10 920.0 11 779.0 12 787.0 13 685.0 14 704.0 15 723.0 16 772.0 17 812.0 18 903.0 19 1089.0 20 1419.0 21 1831.0 22 2322.0 23 2879.0 24 3383.0 25 4017.0 26 4741.0 27 5249.0 28 6394.0 29 7788.0 30 9764.0 31 12675.0 32 16695.0 33 22071.0 34 30127.0 35 42339.0 36 62358.0 37 109674.0 38 264137.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.29915167159541 19.850665380181447 10.35098491303694 28.499198035186208 2 13.771864142336623 21.040643885872544 41.252619937528785 23.934872034262046 3 18.490622139106893 24.98931933239959 29.30070969710722 27.2193488313863 4 11.644172124709366 17.719888971989523 38.78579542431914 31.850143478981973 5 12.27072869235945 39.231407157051436 36.04077672941213 12.457087421176986 6 31.143178328408638 38.925715075220666 17.882140154429244 12.04896644194145 7 27.920380849838494 31.733417632771616 22.11968224954303 18.226519267846857 8 24.147260499047036 39.677775726146926 19.898506864791614 16.276456910014428 9 26.134009326197855 15.668012393202515 20.159139986855696 38.038838293743936 10 14.082303238929745 30.293537787513692 34.889532154592395 20.73462681896417 11 34.031926445874944 22.827001974670395 25.110390646759317 18.030680932695347 12 23.362112668122844 25.302449007691873 32.262061973100344 19.073376351084935 13 29.11593236887316 22.86227496190038 25.590583213636375 22.431209455590082 14 19.70246495952234 23.55167590335431 27.826160427338927 28.91969870978442 15 21.82934519162013 33.27585694324702 23.06138447167358 21.83341339345927 16 20.63500507734939 29.23301513190938 29.35709334656 20.77488644418123 17 20.48291551794817 30.13168147353071 28.611304954640616 20.774098053880504 18 20.345008331833863 28.364222401289286 31.232876712328768 20.057892554548086 19 22.85884609727781 27.46738473959425 27.924733773731923 21.749035389396013 20 22.886768599127542 28.327721415204753 28.886931323891123 19.89857866177658 21 21.63787896332971 27.503966850586654 27.642141567273715 23.216012618809913 22 22.085280026536573 29.77353164830282 27.640592191113377 20.500596134047232 23 20.843444940441266 29.44093178557291 28.578804819774785 21.136818454211046 24 22.456572964616864 27.55969946081944 29.53648622007204 20.44724135449166 25 21.373190565828118 28.7919684672209 28.919495337836675 20.915345629114306 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 150.0 1 150.0 2 162.0 3 174.0 4 174.0 5 174.0 6 361.0 7 548.0 8 548.0 9 548.0 10 900.5 11 1253.0 12 1253.0 13 1253.0 14 2135.0 15 3017.0 16 3017.0 17 3017.0 18 5354.5 19 7692.0 20 7692.0 21 7692.0 22 12334.5 23 16977.0 24 16977.0 25 16977.0 26 24802.5 27 32628.0 28 32628.0 29 32628.0 30 43299.0 31 53970.0 32 53970.0 33 53970.0 34 61453.5 35 68937.0 36 68937.0 37 68937.0 38 74077.0 39 79217.0 40 79217.0 41 79217.0 42 82031.0 43 84845.0 44 84845.0 45 84845.0 46 85369.0 47 85893.0 48 85893.0 49 85893.0 50 80609.5 51 75326.0 52 75326.0 53 75326.0 54 64395.0 55 53464.0 56 53464.0 57 53464.0 58 44844.0 59 36224.0 60 36224.0 61 36224.0 62 28509.5 63 20795.0 64 20795.0 65 20795.0 66 15780.5 67 10766.0 68 10766.0 69 10766.0 70 7687.0 71 4608.0 72 4608.0 73 4608.0 74 3181.5 75 1755.0 76 1755.0 77 1755.0 78 1193.5 79 632.0 80 632.0 81 632.0 82 409.0 83 186.0 84 186.0 85 186.0 86 114.0 87 42.0 88 42.0 89 42.0 90 29.5 91 17.0 92 17.0 93 17.0 94 9.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10733542162793099 2 0.11641042812125461 3 0.01768061609906152 4 0.0 5 0.004381037617466571 6 0.34297265919595443 7 0.01971466927859957 8 0.00938793775171408 9 0.009075006493323611 10 0.0028163813255142243 11 0.0037551751006856325 12 3.1293125839046936E-4 13 6.258625167809387E-4 14 0.0 15 0.00234698443792852 16 7.823281459761735E-4 17 0.0 18 4.6939688758570406E-4 19 0.0 20 3.1293125839046936E-4 21 0.011265525302056897 22 0.0 23 1.5646562919523468E-4 24 0.0 25 0.005789228280223683 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 639118.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.82115637534586 #Duplication Level Percentage of deduplicated Percentage of total 1 92.80295649108302 77.78851128133489 2 5.552042883380853 9.307573094609852 3 0.8270719351410218 2.079783780273465 4 0.279022845842364 0.9355207037458726 5 0.1349672838566497 0.5656556902851964 6 0.07459380051103147 0.37515231703599144 7 0.057515616629543125 0.3374717846870564 8 0.041680641229169244 0.27949756370359063 9 0.028098060558249486 0.2119690735107271 >10 0.1691584739418295 2.637856747161247 >50 0.016072315427202825 0.9220465756103455 >100 0.01476391710662204 2.4944817080985806 >500 7.475401063323916E-4 0.44864328123206726 >1k 0.0013081951860816853 1.6158363987111641 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1816 0.2841415826185462 No Hit CTGTAGGACGTGGAATATGGCAAGA 1803 0.28210752943900813 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1436 0.22468464352435702 No Hit GTCCTACAGTGTGCATTTCTCATTT 1419 0.22202472782803803 No Hit GTATCAACGCAGAGTACTTTTTTTT 1416 0.22155533094045232 No Hit CTTTAGGACGTGAAATATGGCGAGG 1359 0.21263679007632394 No Hit CTGTAGGACCTGGAATATGGCGAGA 1066 0.16679236072212017 No Hit GGTATCAACGCAGAGTACTTTTTTT 863 0.13502983799548754 No Hit TATCAACGCAGAGTACTTTTTTTTT 763 0.11938327507596405 No Hit CTGAAGGACCTGGAATATGGCGAGA 690 0.10796128414471193 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGGAC 80 7.4342097E-9 15.476491 3 TAGGACC 360 0.0 14.549405 4 CTATACC 60 2.5051593E-5 14.28487 4 GGCGAGG 340 0.0 13.115047 19 TGTAGGA 975 0.0 13.004984 2 TAGGACT 110 2.6357156E-9 12.986245 4 CTCCTAG 60 3.9657566E-4 12.711638 1 GTCTAGA 75 1.4225399E-5 12.7116375 1 CTATTCT 75 1.4387293E-5 12.697661 4 GTAGGAC 930 0.0 12.493843 3 GACGTGG 380 0.0 12.486459 7 ATACTTA 55 0.0030094313 12.120496 6 CTGTAGG 940 0.0 12.069295 1 GTCCTAA 415 0.0 11.945877 1 AGGACCT 610 0.0 11.865029 5 GGACCTG 555 0.0 11.839713 6 GTGTAGG 130 2.4592737E-9 11.733821 1 CTAAATT 510 0.0 11.7198305 19 GGACGTG 780 0.0 11.598826 6 AGGACGT 805 0.0 11.593517 5 >>END_MODULE