FastQCFastQC Report
Thu 26 May 2016
SRR1926959_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926959_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625693
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17790.2843247407274814No Hit
CTGTAGGACGTGGAATATGGCAAGA14380.22982516985166848No Hit
GTCCTACAGTGTGCATTTCTCATTT13360.21352324542547224No Hit
GTCCTAAAGTGTGTATTTCTCATTT11410.18235780166950885No Hit
GTATCAACGCAGAGTACTTTTTTTT9340.14927448445164002No Hit
CTGTAGGACCTGGAATATGGCGAGA9070.14495926916235277No Hit
CTTTAGGACGTGAAATATGGCGAGG8710.13920564877663646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT851.6287231E-814.5831871
GCCGGTT400.005330572414.22727711
CCGGTTT400.005330572414.22727712
TAGGACC2950.013.2278074
TAGGACT801.9529925E-613.0866574
GTACTAA905.151487E-712.7135471
CCTGGAC603.9793656E-412.7063993
AGGACGT5900.012.4182445
AATGTCC1800.012.1486678
GGACGTG5800.012.14018256
GTCGTCA550.002981247812.1356581
TCGTCAA550.002993837212.1288352
ACCAACT550.003046480712.1006498
GTAGGAC8000.012.031373
ATTTAGA1750.011.9870591
CTGTAGG8400.011.918951
GACGTGG3600.011.8940977
ATGTCCA1850.011.792929
TGTAGGA8450.011.7289842
GGCGAGG2350.011.7047119
Produced by FastQC (version 0.11.5)