##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926959_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 625693 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.349099318675453 31.0 28.0 31.0 22.0 34.0 2 28.39539039113431 31.0 28.0 31.0 20.0 34.0 3 27.979496334464347 31.0 28.0 31.0 16.0 34.0 4 31.794188204119273 35.0 32.0 37.0 19.0 37.0 5 31.645887040449548 35.0 32.0 37.0 19.0 37.0 6 31.27745555727809 35.0 32.0 37.0 17.0 37.0 7 31.115679734310596 35.0 32.0 37.0 17.0 37.0 8 31.248762572060098 35.0 32.0 37.0 17.0 37.0 9 33.404193430324455 37.0 33.0 39.0 18.0 39.0 10 33.57499764261387 38.0 34.0 39.0 19.0 39.0 11 33.72408033971932 38.0 34.0 39.0 22.0 39.0 12 33.61095297534094 38.0 34.0 39.0 19.0 39.0 13 33.709416598875166 38.0 34.0 39.0 22.0 39.0 14 34.74290586597581 39.0 35.0 41.0 22.0 41.0 15 34.35850329155033 39.0 34.0 41.0 17.0 41.0 16 34.436854815380705 39.0 34.0 41.0 17.0 41.0 17 34.657904115916274 39.0 34.0 41.0 19.0 41.0 18 34.6957085983062 39.0 35.0 41.0 19.0 41.0 19 34.11347098337364 39.0 34.0 41.0 11.0 41.0 20 34.416296810096966 39.0 34.0 41.0 18.0 41.0 21 34.3958362967142 39.0 34.0 41.0 18.0 41.0 22 34.41610022806712 39.0 34.0 41.0 18.0 41.0 23 34.39787403726748 39.0 34.0 41.0 16.0 41.0 24 33.15572972687884 39.0 33.0 41.0 2.0 41.0 25 33.708879594305834 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.09165256868675442 1101 2 -0.14561588312277252 1101 3 -1.1242378592264757 1101 4 -0.49718575116844477 1101 5 -0.4508887172460163 1101 6 -2.097531281340796 1101 7 -0.6176543638802805 1101 8 0.05453329114102701 1101 9 -0.07894894496263305 1101 10 -8.55173100206283 1101 11 -3.501748838792974 1101 12 -1.5043160648388252 1101 13 -0.7860613409107984 1101 14 -0.4444520830265688 1101 15 -1.4610955095282492 1101 16 -0.15349507099785598 1101 17 -0.08526227761208105 1101 18 -0.04861377376462883 1101 19 -0.545722804494325 1101 20 0.0617864816552256 1101 21 -0.0024404216164057857 1101 22 0.22932768949617355 1101 23 0.1265042088381918 1101 24 -0.055243873554687184 1101 25 0.09392351526293652 1102 1 0.020166082200269386 1102 2 -0.17305068634705023 1102 3 -0.4004256259405601 1102 4 -0.05557124192235818 1102 5 0.04331351129831873 1102 6 -0.7065841499989283 1102 7 0.09779845736201409 1102 8 0.6663813233837956 1102 9 0.7429144974271225 1102 10 0.545592373944757 1102 11 0.29452783261528026 1102 12 0.22038284459692647 1102 13 -0.027905816965798635 1102 14 -0.033711210575170014 1102 15 0.48883810830010077 1102 16 0.362169936114519 1102 17 0.2774878409275132 1102 18 -0.04452652862475759 1102 19 0.4465519702804457 1102 20 0.2602193882460284 1102 21 0.5063635138980587 1102 22 0.24348368759954298 1102 23 -0.03622694336664978 1102 24 -3.2374819010558724 1102 25 -1.5924752519253005 1103 1 -0.1541796102751931 1103 2 -0.23630997961839384 1103 3 -0.037170159029006555 1103 4 -0.039923821845448515 1103 5 -0.28767258944973406 1103 6 0.3665531302245313 1103 7 0.188229688110642 1103 8 0.38437501945603714 1103 9 0.41591857250030273 1103 10 0.5207521244660995 1103 11 0.16125213382632353 1103 12 -0.19222788068957897 1103 13 -0.024170333218258122 1103 14 0.04063854740757478 1103 15 0.4882976349793964 1103 16 0.36737498116990963 1103 17 0.21006738138170533 1103 18 0.15244490759939566 1103 19 -1.6777891783927643 1103 20 -0.27704696465913514 1103 21 -0.15760915632712624 1103 22 0.003028063807235526 1103 23 0.0279985057523362 1103 24 1.5686460141519945 1103 25 0.8384948259411189 1104 1 -0.45408685269834237 1104 2 -0.5039406112660707 1104 3 -1.739244926058518 1104 4 -0.5361012087818757 1104 5 -0.9082033210707579 1104 6 -2.043473765981254 1104 7 -0.7275727347591641 1104 8 -0.11696862699909971 1104 9 -0.07488306478148843 1104 10 0.014223597146838074 1104 11 -0.03212006380667276 1104 12 0.023136125879105407 1104 13 -0.18251927162699388 1104 14 -0.4319036968602532 1104 15 0.07530761718437162 1104 16 -0.33274454459098024 1104 17 -0.15594098193005834 1104 18 -0.4083322542515191 1104 19 -1.2184471403563393 1104 20 -0.5075140461938616 1104 21 -0.2666510997438394 1104 22 -0.30773193149087774 1104 23 -0.32067338497761 1104 24 1.0240938633284102 1104 25 0.2410336136217026 1105 1 -0.05116899633376093 1105 2 -0.12559041262609583 1105 3 0.04261653443419888 1105 4 -0.06855691290610011 1105 5 -0.11922322976900901 1105 6 -0.17697659052432613 1105 7 -0.19355361391565395 1105 8 0.36736759140988084 1105 9 -0.6731643121622923 1105 10 -0.15565911866110582 1105 11 -0.2822667388920266 1105 12 -0.3519006127375093 1105 13 -0.5019804013324745 1105 14 -0.5529394185296113 1105 15 0.0965215840050746 1105 16 -0.4245860572720872 1105 17 -0.49580140312502863 1105 18 -0.2927399942259328 1105 19 -1.1460963717788886 1105 20 -0.29013654084371154 1105 21 -0.3950200169185152 1105 22 -0.2895855693123721 1105 23 -0.40701779484088263 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1108 22 0.6595472840907703 1108 23 0.8989704250544079 1108 24 -0.36653891859972987 1108 25 0.37452036210978434 1109 1 0.21262231289027866 1109 2 0.2997572345881423 1109 3 -1.1863760839599635 1109 4 0.10088032726838492 1109 5 0.40843379785405176 1109 6 0.6841359884006231 1109 7 0.6429273713049568 1109 8 0.9647567999227249 1109 9 0.40399368871120345 1109 10 0.4897876329470492 1109 11 0.16525592085854157 1109 12 -0.009787715686378817 1109 13 0.010014034155311435 1109 14 0.02798702961851518 1109 15 -1.4494196111788185 1109 16 -0.41628350309627393 1109 17 -0.4351072410100798 1109 18 -0.2833018913363716 1109 19 -1.9773881258403918 1109 20 -0.8132556871871515 1109 21 -0.6927947512292292 1109 22 -0.27070181477638044 1109 23 -0.28735166557381575 1109 24 -1.2399662272162786 1109 25 -0.7680109629846186 1110 1 0.535176173017625 1110 2 0.6070314564468688 1110 3 1.1245624244073085 1110 4 0.6944885063383062 1110 5 0.8783031991911088 1110 6 0.8157738704305473 1110 7 1.0594754803938606 1110 8 1.2711476855556256 1110 9 1.0138396660499964 1110 10 1.0649663006889654 1110 11 0.8329957544379951 1110 12 0.8632165939551797 1110 13 0.6083423463284134 1110 14 0.6440122036617382 1110 15 1.172964544471256 1110 16 0.7256551185458804 1110 17 0.3055119880764181 1110 18 0.5060691836630298 1110 19 -1.6944461797363672 1110 20 0.38833514939460656 1110 21 0.41874047673906745 1110 22 0.5287860580564612 1110 23 0.457957559262276 1110 24 -0.670917912881599 1110 25 -0.3590210943315171 1111 1 0.712911279369127 1111 2 0.4795933098922873 1111 3 1.1793732139295905 1111 4 1.0351969449756275 1111 5 1.0411704197473952 1111 6 1.179998300819726 1111 7 1.242845731766426 1111 8 1.657446416602184 1111 9 1.0814675281333166 1111 10 0.7336722171320957 1111 11 0.5955462722938449 1111 12 0.6741683447477058 1111 13 0.5170403452040375 1111 14 0.8875753539149258 1111 15 -0.20098237313604272 1111 16 0.2498388999802046 1111 17 0.5178333964239386 1111 18 0.6258387171489304 1111 19 1.1902113905703047 1111 20 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14 -2.3660185265967897 2216 15 -3.3507383849178183 2216 16 -2.3093117572493256 2216 17 -2.603303456787472 2216 18 -2.5252581307870585 2216 19 -1.8646090253087166 2216 20 -2.588796464239632 2216 21 -4.9671693849843095 2216 22 -3.8681760145859805 2216 23 -3.4780735257962583 2216 24 -3.7522233861485645 2216 25 -3.0389523852122053 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33842.0 3 5.0 4 264.0 5 1526.0 6 1899.0 7 1575.0 8 1208.0 9 1155.0 10 1117.0 11 1070.0 12 1048.0 13 1030.0 14 1037.0 15 1092.0 16 1166.0 17 1197.0 18 1531.0 19 1787.0 20 2415.0 21 3178.0 22 4061.0 23 4627.0 24 5538.0 25 6405.0 26 7346.0 27 7880.0 28 8859.0 29 10487.0 30 12649.0 31 15373.0 32 20034.0 33 26294.0 34 35900.0 35 50479.0 36 74307.0 37 119482.0 38 156830.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.666613271394816 19.100076088262384 12.904409114572903 25.328901525769894 2 13.511229616162069 20.29221168942785 43.83959566019708 22.356963034213 3 19.714378542059784 24.3930584030742 29.11751195957831 26.77505109528771 4 11.251187593368032 17.229140742209868 39.573330091776576 31.946341572645526 5 12.026121848672327 38.517736959058716 36.647642798906524 12.808498393362427 6 30.530928181140954 38.983543975982585 18.552112874193107 11.933414968683358 7 27.29394677315669 31.32659915387242 22.44626737182642 18.933186701144468 8 23.366782617059133 39.179584181587934 21.586650209191607 15.866982992161327 9 25.6201213946477 16.260872206316552 20.840426943409376 37.27857945562637 10 13.629503143326746 30.34493454333826 35.77860491292697 20.246957400408025 11 33.780223855545714 23.11575460930781 25.002637543919104 18.10138399122737 12 22.69615216769266 25.42676873842062 32.819511072248964 19.057568021637763 13 28.557635745159722 22.946079183610834 25.949672625898362 22.546612445331082 14 19.12969250541904 23.827473096621972 28.53756242287059 28.505271975088398 15 22.027187309178913 33.22708628390021 23.329326423545286 21.416399983375587 16 20.269198797877102 29.566308587505596 29.953481680414352 20.211010934202957 17 20.210339199534495 30.001870321199693 29.203546896305877 20.584243582959935 18 19.864351617997787 28.92782470251987 31.596833698856113 19.610989980626226 19 22.795280185058182 27.7635232673576 28.107709745129355 21.333486802454864 20 22.161584093840965 28.720572790531502 29.56348540288921 19.554357712738323 21 21.460895975702353 28.003037205770692 27.99232705910562 22.54373975942133 22 21.384405851961738 30.073629085886743 28.451423346462732 20.09054171568878 23 20.69962689549557 29.445362729833334 28.969790364504238 20.885220010166858 24 22.177161527935855 28.084837014207707 29.72748315152755 20.010518306328887 25 21.151774210565016 28.75103504941062 29.42321771684885 20.673973023175517 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 177.0 1 177.0 2 242.5 3 308.0 4 308.0 5 308.0 6 484.0 7 660.0 8 660.0 9 660.0 10 1014.0 11 1368.0 12 1368.0 13 1368.0 14 2430.0 15 3492.0 16 3492.0 17 3492.0 18 5992.0 19 8492.0 20 8492.0 21 8492.0 22 13390.0 23 18288.0 24 18288.0 25 18288.0 26 26356.5 27 34425.0 28 34425.0 29 34425.0 30 45169.0 31 55913.0 32 55913.0 33 55913.0 34 63325.5 35 70738.0 36 70738.0 37 70738.0 38 75089.0 39 79440.0 40 79440.0 41 79440.0 42 81452.5 43 83465.0 44 83465.0 45 83465.0 46 83603.0 47 83741.0 48 83741.0 49 83741.0 50 77368.5 51 70996.0 52 70996.0 53 70996.0 54 60415.0 55 49834.0 56 49834.0 57 49834.0 58 40776.5 59 31719.0 60 31719.0 61 31719.0 62 24805.5 63 17892.0 64 17892.0 65 17892.0 66 13395.5 67 8899.0 68 8899.0 69 8899.0 70 6286.0 71 3673.0 72 3673.0 73 3673.0 74 2556.5 75 1440.0 76 1440.0 77 1440.0 78 980.5 79 521.0 80 521.0 81 521.0 82 328.0 83 135.0 84 135.0 85 135.0 86 96.5 87 58.0 88 58.0 89 58.0 90 35.5 91 13.0 92 13.0 93 13.0 94 7.0 95 1.0 96 1.0 97 1.0 98 3.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22662871408182608 2 0.13936547156512857 3 0.14064405387306556 4 0.07655511568772545 5 0.02605111452421555 6 0.02349394990834163 7 0.30526152601994905 8 0.2977498549608194 9 0.02077696250397559 10 0.038517292026600904 11 0.017740329522625314 12 0.019178734619054394 13 0.01742068394564107 14 0.019338557407546513 15 0.017101038368656833 16 0.02125643086945195 17 0.021096608080959832 18 0.016781392791672595 19 0.025571646158739192 20 0.01981802577302287 21 0.018859089042070152 22 0.01997784856151499 23 0.020936785292467713 24 0.018699266253578033 25 0.019018911830562275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 625693.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.62104769468785 #Duplication Level Percentage of deduplicated Percentage of total 1 94.95436409795975 82.25046501348127 2 3.8227199070243607 6.622560067795797 3 0.5326229980508456 1.384090863523498 4 0.2124705129524263 0.7361767374466764 5 0.11281870907706396 0.4886237389908735 6 0.07686742959135649 0.3995002370880571 7 0.04632523762346362 0.28089184323548533 8 0.034196004305473336 0.23696749759297256 9 0.023852275049919547 0.18594981492533047 >10 0.15492487051157722 2.5895376538111945 >50 0.013864475633817042 0.8569997599997597 >100 0.013494619778775693 2.5363921585189817 >500 7.394312206061255E-4 0.5200884930597633 >1k 7.394312206061255E-4 0.9117561205302626 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1779 0.2843247407274814 No Hit CTGTAGGACGTGGAATATGGCAAGA 1438 0.22982516985166848 No Hit GTCCTACAGTGTGCATTTCTCATTT 1336 0.21352324542547224 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1141 0.18235780166950885 No Hit GTATCAACGCAGAGTACTTTTTTTT 934 0.14927448445164002 No Hit CTGTAGGACCTGGAATATGGCGAGA 907 0.14495926916235277 No Hit CTTTAGGACGTGAAATATGGCGAGG 871 0.13920564877663646 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 1.5982278849211993E-4 9 0.0 0.0 0.0 1.5982278849211993E-4 1.5982278849211993E-4 10 0.0 0.0 0.0 1.5982278849211993E-4 1.5982278849211993E-4 11 0.0 0.0 0.0 1.5982278849211993E-4 1.5982278849211993E-4 12 0.0 0.0 0.0 1.5982278849211993E-4 1.5982278849211993E-4 13 0.0 0.0 0.0 1.5982278849211993E-4 1.5982278849211993E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAT 85 1.6287231E-8 14.583187 1 GCCGGTT 40 0.0053305724 14.227277 11 CCGGTTT 40 0.0053305724 14.227277 12 TAGGACC 295 0.0 13.227807 4 TAGGACT 80 1.9529925E-6 13.086657 4 GTACTAA 90 5.151487E-7 12.713547 1 CCTGGAC 60 3.9793656E-4 12.706399 3 AGGACGT 590 0.0 12.418244 5 AATGTCC 180 0.0 12.148667 8 GGACGTG 580 0.0 12.1401825 6 GTCGTCA 55 0.0029812478 12.135658 1 TCGTCAA 55 0.0029938372 12.128835 2 ACCAACT 55 0.0030464807 12.100649 8 GTAGGAC 800 0.0 12.03137 3 ATTTAGA 175 0.0 11.987059 1 CTGTAGG 840 0.0 11.91895 1 GACGTGG 360 0.0 11.894097 7 ATGTCCA 185 0.0 11.79292 9 TGTAGGA 845 0.0 11.728984 2 GGCGAGG 235 0.0 11.70471 19 >>END_MODULE