FastQCFastQC Report
Thu 26 May 2016
SRR1926959_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926959_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625693
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17060.2726576771675566No Hit
CTGTAGGACGTGGAATATGGCAAGA16720.26722370235882453No Hit
GTATCAACGCAGAGTACTTTTTTTT15110.24149223341159323No Hit
GTCCTACAGTGTGCATTTCTCATTT13560.21671970119531464No Hit
CTGTAGGACCTGGAATATGGCGAGA11610.18555425743935125No Hit
CTTTAGGACGTGAAATATGGCGAGG11310.18075957378458765No Hit
GTCCTAAAGTGTGTATTTCTCATTT11140.1780425863802216No Hit
GGTATCAACGCAGAGTACTTTTTTT10770.1721291432060132No Hit
TATCAACGCAGAGTACTTTTTTTTT8640.13808688925719162No Hit
CACACACACACACACACACACACAC6300.10068835675003555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG551.1371276E-515.52956418
TTTAGGG852.6223825E-713.4399513
TAGGACC3200.013.38530354
AGGACCT4950.012.6912525
GGACCTG4800.012.6912526
TAGGCAG604.0196336E-412.6912525
GTCTTAA853.7934442E-612.3337991
ACTGTTC2250.012.230958
TCTCAAC550.003017091212.1163193
GGCGAGG3850.012.07854819
GATATAC2550.011.9600481
GCTCTAG1209.498763E-911.9133291
AGACTCC657.882344E-411.7150016
TGTAGGA10200.011.4910142
GTCCTAA4400.011.4801171
AGGACGT8650.011.4441345
TGGCGAG8300.011.43408218
CTAAATT4400.011.43148419
GTAGGAC9350.011.3017773
CTAGTAC855.1984236E-511.1999593
Produced by FastQC (version 0.11.5)