##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926959_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 625693 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.01212735319078 31.0 30.0 33.0 26.0 34.0 2 30.304165141690895 31.0 30.0 34.0 26.0 34.0 3 30.37554040080359 31.0 30.0 34.0 26.0 34.0 4 33.93042594371361 37.0 35.0 37.0 30.0 37.0 5 33.900782012904095 37.0 35.0 37.0 30.0 37.0 6 33.85250434318428 37.0 35.0 37.0 30.0 37.0 7 33.85326989434115 37.0 35.0 37.0 29.0 37.0 8 33.88908458301435 37.0 35.0 37.0 30.0 37.0 9 35.42975708534377 39.0 35.0 39.0 30.0 39.0 10 35.34219817066836 39.0 35.0 39.0 30.0 39.0 11 35.37413715672063 39.0 35.0 39.0 30.0 39.0 12 35.26733717653865 39.0 35.0 39.0 30.0 39.0 13 35.298376360291705 39.0 35.0 39.0 30.0 39.0 14 36.4079620516771 40.0 37.0 41.0 30.0 41.0 15 36.399986894531345 40.0 37.0 41.0 30.0 41.0 16 36.38951051074569 40.0 37.0 41.0 30.0 41.0 17 36.35885170522924 40.0 37.0 41.0 30.0 41.0 18 36.33550798874208 40.0 37.0 41.0 29.0 41.0 19 36.408093106363665 40.0 37.0 41.0 30.0 41.0 20 36.37466776837842 40.0 37.0 41.0 29.0 41.0 21 36.32140842234131 40.0 37.0 41.0 29.0 41.0 22 36.242590215968534 40.0 37.0 41.0 29.0 41.0 23 36.16849796945147 40.0 37.0 41.0 29.0 41.0 24 36.10973272835081 40.0 37.0 41.0 29.0 41.0 25 35.99230293450622 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06670513601583394 1101 2 0.01650637657706966 1101 3 0.22362821147205025 1101 4 0.07353835440837031 1101 5 0.24780195754760825 1101 6 0.33110885048817096 1101 7 0.333403106859123 1101 8 0.23840747401715134 1101 9 0.2764093761259474 1101 10 0.31299700464943214 1101 11 0.4825039248539298 1101 12 0.1580897619594026 1101 13 0.2937402394407016 1101 14 0.34850875604238496 1101 15 0.17836062496565575 1101 16 0.40924885337037153 1101 17 0.3683281472529245 1101 18 0.3077327821467435 1101 19 0.36859598513191116 1101 20 0.25443487285551925 1101 21 0.2987086380239319 1101 22 0.048931545116808195 1101 23 0.07394399260522277 1101 24 0.18861124874952395 1101 25 0.2256932417143105 1102 1 -0.7112488706223843 1102 2 -0.5988041971545535 1102 3 -0.3141337610267634 1102 4 -0.26113803250486 1102 5 -0.4796233625092938 1102 6 -0.5109549238124487 1102 7 -0.34508314254818373 1102 8 -0.46299484935885715 1102 9 -0.40680304682332036 1102 10 -0.6405994865786369 1102 11 -0.45514543503227145 1102 12 -0.43047590897469235 1102 13 -0.49584487198651317 1102 14 -0.5340339182108167 1102 15 -0.4388726609518372 1102 16 -0.6587419005414361 1102 17 -0.3608906768343232 1102 18 -0.43246399357634857 1102 19 -0.4627375852806068 1102 20 -0.599966502203749 1102 21 -0.509593875015419 1102 22 -0.6939880850396634 1102 23 -0.6094545375038365 1102 24 -0.5421853373102223 1102 25 -0.4172738042790485 1103 1 -0.6483315582575031 1103 2 -0.6668512231164065 1103 3 -0.4877644025159853 1103 4 -0.6081828870075299 1103 5 -0.738046137002776 1103 6 -0.5846446347309637 1103 7 -0.5964044324140687 1103 8 -0.8206841585096001 1103 9 -0.7339687664659351 1103 10 -0.7370357107267935 1103 11 -0.7370321652165117 1103 12 -0.9638106771929458 1103 13 -0.8852558995554318 1103 14 -1.0074203901157688 1103 15 -0.956471524522982 1103 16 -0.9275568290841676 1103 17 -1.0051623770772977 1103 18 -0.8570583994383298 1103 19 -1.051201827948347 1103 20 -0.9649856818322107 1103 21 -0.8607054325351768 1103 22 -1.0000024051911893 1103 23 -0.9427555859264203 1103 24 -0.9246252156319841 1103 25 -1.0582667924747255 1104 1 0.25404021923138487 1104 2 0.08743314112176392 1104 3 0.29728940735309806 1104 4 0.5529487787067353 1104 5 0.47889486475578025 1104 6 0.43795935093525884 1104 7 0.6513863195274965 1104 8 0.5860921490870936 1104 9 0.5419111212007977 1104 10 0.5150460974989528 1104 11 0.5753234466168919 1104 12 0.6748176745037497 1104 13 0.4460446035700443 1104 14 0.515617123748406 1104 15 0.7017988550847818 1104 16 0.5979107703567266 1104 17 0.5910989222969079 1104 18 0.6809134334490565 1104 19 0.643870149370855 1104 20 0.5658681417701104 1104 21 0.5012423136419244 1104 22 0.689068843567874 1104 23 0.7137201615169317 1104 24 0.7070282783725261 1104 25 0.5379526088722955 1105 1 0.1063202485105279 1105 2 0.11718275976654269 1105 3 0.390697689326867 1105 4 0.4698932433287055 1105 5 0.40417382679905955 1105 6 0.30524214608743705 1105 7 -0.045784667302477544 1105 8 0.09071255396620614 1105 9 0.26849088171530866 1105 10 0.382944648628488 1105 11 0.302325631377208 1105 12 0.44493283539933515 1105 13 0.5207276456951249 1105 14 0.34636357421133823 1105 15 0.40627062921073787 1105 16 0.3076159115280035 1105 17 0.3791559383948453 1105 18 0.3670790393987602 1105 19 0.3762710947965502 1105 20 0.5162489382297935 1105 21 0.43371925073088136 1105 22 0.47932067770489795 1105 23 0.17098658493576835 1105 24 0.2377127062279385 1105 25 0.4298477627274764 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1107 13 0.4142693486102971 1107 14 0.333694787995789 1107 15 0.18938699877148935 1107 16 0.23475931851830012 1107 17 0.32721222770799585 1107 18 0.41192700658265835 1107 19 0.30778435779460267 1107 20 0.4812964171793013 1107 21 0.4034951163451552 1107 22 0.6047378650318933 1107 23 0.4361442593372118 1107 24 0.3624497398085538 1107 25 0.30557892800483444 1108 1 0.7882028837635815 1108 2 0.6203295747752691 1108 3 0.6513815898504269 1108 4 0.709585651705666 1108 5 0.8350147953854474 1108 6 0.808727093731413 1108 7 0.7407172960483095 1108 8 0.6159525190621906 1108 9 0.5503789707205442 1108 10 0.6709136713161001 1108 11 0.5322449158449203 1108 12 0.7142609331305749 1108 13 0.6288810052064733 1108 14 0.5645954677540956 1108 15 0.5949540934341258 1108 16 0.7728918713883886 1108 17 0.7963461652709398 1108 18 0.764630304669268 1108 19 0.7233369761229014 1108 20 0.6690576206032688 1108 21 0.6830048091951042 1108 22 0.9173605991708627 1108 23 0.7902165151277458 1108 24 0.8631326451459174 1108 25 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2211 13 0.21464420034466514 2211 14 0.1503836379172725 2211 15 0.1939353205403549 2211 16 0.37586493998645665 2211 17 0.18317600210077956 2211 18 0.28533355974752084 2211 19 0.3721610686969967 2211 20 0.33084062926127444 2211 21 0.33191111122641104 2211 22 0.24052805671332322 2211 23 0.24674956541079496 2211 24 0.4128464729847465 2211 25 0.51112564714672 2212 1 0.07517499852824727 2212 2 -0.09487634076309348 2212 3 0.0027415995216095723 2212 4 0.15950102888168516 2212 5 -0.022329256838922618 2212 6 -0.012301678028741492 2212 7 -0.20781389350681678 2212 8 -0.3400033158830027 2212 9 -0.17301924011118075 2212 10 -0.3134633435130425 2212 11 -0.2061698165857706 2212 12 -0.4036468682878649 2212 13 -0.29158361439378666 2212 14 -0.20320957737807532 2212 15 -0.25431855324543307 2212 16 -0.15437078173862773 2212 17 -0.3870516425949546 2212 18 -0.3427298661882432 2212 19 -0.047621276617263675 2212 20 -0.14235638648893456 2212 21 -0.2347881469622095 2212 22 -0.2160472828407407 2212 23 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0.5429392011812268 2214 11 0.37900551314742614 2214 12 0.5651761547105352 2214 13 0.521432787480073 2214 14 0.6362898319159456 2214 15 0.4610487466133151 2214 16 0.42287380780457084 2214 17 0.4282344118990693 2214 18 0.6208140715286135 2214 19 0.48960624394563723 2214 20 0.5765178807188747 2214 21 0.5761225837615811 2214 22 0.7136214373211516 2214 23 0.6049443571316004 2214 24 0.3701714216391707 2214 25 0.5255755517700322 2215 1 0.1645786699271028 2215 2 0.29306935464948225 2215 3 0.13445942003910716 2215 4 0.28045829321509785 2215 5 0.42375300293261375 2215 6 0.20454077297669926 2215 7 -0.09967185489508523 2215 8 0.13678585239552632 2215 9 0.17024925373941358 2215 10 0.2560158725739612 2215 11 0.10909240012165355 2215 12 0.10121061866516357 2215 13 0.18239908696747875 2215 14 0.09877392687359787 2215 15 -0.00235329650298155 2215 16 -0.1669469650896005 2215 17 0.033453083509932924 2215 18 -0.12383274564520264 2215 19 -0.15783440752489497 2215 20 -0.10108781964830627 2215 21 0.038999306050449434 2215 22 -0.034938929131023144 2215 23 -0.08214983707351564 2215 24 -0.09769675736980332 2215 25 -0.06312368842337435 2216 1 -0.2028376014286053 2216 2 -0.04989529920078439 2216 3 -1.8258573042894568 2216 4 -1.2494289985778835 2216 5 -0.39609933504933537 2216 6 -0.24152692328181757 2216 7 -0.08598993457550819 2216 8 -0.08911206820938844 2216 9 -0.380640641755825 2216 10 -0.2693470407191896 2216 11 -0.33993923664404946 2216 12 -0.26203081230105596 2216 13 -0.24010332056542438 2216 14 -0.010012676543318833 2216 15 -0.16518098848143836 2216 16 -0.1731994172064475 2216 17 -0.014082316140530793 2216 18 -0.1423974993634971 2216 19 -0.26609670377627737 2216 20 -0.09779217460782519 2216 21 -0.24957828784333458 2216 22 -0.019471867747853366 2216 23 -0.15671558947212816 2216 24 -0.4143622351313354 2216 25 -0.42907403974484026 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14285.0 3 7.0 4 266.0 5 1513.0 6 2044.0 7 1534.0 8 1173.0 9 1038.0 10 873.0 11 855.0 12 740.0 13 791.0 14 737.0 15 756.0 16 801.0 17 865.0 18 861.0 19 1052.0 20 1388.0 21 1861.0 22 2323.0 23 2921.0 24 3301.0 25 4044.0 26 4898.0 27 5373.0 28 6095.0 29 7600.0 30 9717.0 31 12305.0 32 16083.0 33 21275.0 34 29275.0 35 40937.0 36 60948.0 37 105873.0 38 259285.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.187680914762645 20.024994039513626 10.777999484757956 28.009325560965774 2 13.355212522021867 20.9912200554607 41.951557492075324 23.702009930442102 3 18.669695293692634 24.804263522521282 29.10567052331734 27.420370660468745 4 11.563977861347338 17.628773216257812 39.01705788621576 31.79019103617908 5 12.198279582266464 39.07253437159361 36.05876655446551 12.670419491674418 6 30.90663262196589 38.92916371288256 17.973869836758656 12.190333828392895 7 27.97850180883427 31.81821815036904 21.681712309824615 18.521567730972073 8 23.837975039639918 40.2167727993453 19.7312477622628 16.21400439875198 9 25.648984784961105 16.27828544301673 20.41283120561481 37.65989856640736 10 13.94732930452753 30.965970389715835 34.629935380341756 20.45676492541488 11 33.7102159483041 23.24588953407527 24.850597529964404 18.193296987656215 12 22.909268167942592 25.573846473493262 32.39080055618597 19.126084802378173 13 28.756710623682448 22.89707153896437 26.003417043985248 22.342800793367932 14 19.435920548768404 24.05672439475013 28.025929690646517 28.481425365834944 15 21.64722880628575 33.3757945495142 23.343173467120796 21.633803177079255 16 20.385305089134167 29.834453703614912 29.593598066761924 20.186643140489 17 20.191851275305943 30.418751688128204 28.761069725887932 20.62832731067792 18 19.806964802002273 29.065558128718898 31.45204726309716 19.675429806181665 19 22.354556337093985 27.93432519989963 28.259406829910706 21.451711633095673 20 22.283062111026936 28.79479483319109 29.39877190795383 19.523371147828144 21 21.30781993778832 27.890397470612495 28.16597027522115 22.63581231637804 22 21.548267281238562 30.016637552282027 28.238609030307195 20.19648613617221 23 20.730806850654954 29.649252347800513 28.642686817156044 20.977253984388486 24 21.884569404755055 28.077073064702763 29.8811555845368 20.157201946005383 25 21.121495028346658 29.037939924590066 29.320527390045825 20.520037657017458 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 205.0 1 205.0 2 221.5 3 238.0 4 238.0 5 238.0 6 429.5 7 621.0 8 621.0 9 621.0 10 974.5 11 1328.0 12 1328.0 13 1328.0 14 2244.5 15 3161.0 16 3161.0 17 3161.0 18 5572.0 19 7983.0 20 7983.0 21 7983.0 22 12737.5 23 17492.0 24 17492.0 25 17492.0 26 25402.5 27 33313.0 28 33313.0 29 33313.0 30 44024.0 31 54735.0 32 54735.0 33 54735.0 34 62250.0 35 69765.0 36 69765.0 37 69765.0 38 74159.0 39 78553.0 40 78553.0 41 78553.0 42 81138.0 43 83723.0 44 83723.0 45 83723.0 46 84394.0 47 85065.0 48 85065.0 49 85065.0 50 79212.5 51 73360.0 52 73360.0 53 73360.0 54 61943.0 55 50526.0 56 50526.0 57 50526.0 58 41521.0 59 32516.0 60 32516.0 61 32516.0 62 25407.0 63 18298.0 64 18298.0 65 18298.0 66 13604.0 67 8910.0 68 8910.0 69 8910.0 70 6392.0 71 3874.0 72 3874.0 73 3874.0 74 2595.0 75 1316.0 76 1316.0 77 1316.0 78 918.0 79 520.0 80 520.0 81 520.0 82 331.5 83 143.0 84 143.0 85 143.0 86 90.0 87 37.0 88 37.0 89 37.0 90 23.0 91 9.0 92 9.0 93 9.0 94 5.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11890815463813723 2 0.11922780021512147 3 0.017101038368656833 4 0.0 5 0.004954506443255718 6 0.35145031189417175 7 0.02429306385080223 8 0.009749190098019316 9 0.009909012886511435 10 0.005114329231747838 11 0.005433974808732078 12 4.7946836547635977E-4 13 9.589367309527195E-4 14 1.5982278849211993E-4 15 0.003835746923810878 16 0.0011187595194448396 17 0.0 18 6.392911539684797E-4 19 6.392911539684797E-4 20 0.0011187595194448396 21 0.013904582598814436 22 0.0 23 1.5982278849211993E-4 24 1.5982278849211993E-4 25 0.007351848270637517 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 625693.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.26118896853987 #Duplication Level Percentage of deduplicated Percentage of total 1 93.68304357899284 78.9384463815747 2 4.865344974242679 8.199195045435962 3 0.6759704318904433 1.7087421689599842 4 0.2416036879641792 0.8143125602818337 5 0.11542385624931112 0.48628756814503926 6 0.08382713039187252 0.42380242047840005 7 0.061543997715768156 0.3630039294985408 8 0.04525689868571307 0.3050720073829546 9 0.03264313480516355 0.2475494415309066 >10 0.1611310356099141 2.7109249734859366 >50 0.018056012919474052 1.0762035640266918 >100 0.01330433220367515 2.3271165038696897 >500 0.0013304332202005989 0.6812713238480406 >1k 0.0015204951088006844 1.7180721114813775 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1706 0.2726576771675566 No Hit CTGTAGGACGTGGAATATGGCAAGA 1672 0.26722370235882453 No Hit GTATCAACGCAGAGTACTTTTTTTT 1511 0.24149223341159323 No Hit GTCCTACAGTGTGCATTTCTCATTT 1356 0.21671970119531464 No Hit CTGTAGGACCTGGAATATGGCGAGA 1161 0.18555425743935125 No Hit CTTTAGGACGTGAAATATGGCGAGG 1131 0.18075957378458765 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1114 0.1780425863802216 No Hit GGTATCAACGCAGAGTACTTTTTTT 1077 0.1721291432060132 No Hit TATCAACGCAGAGTACTTTTTTTTT 864 0.13808688925719162 No Hit CACACACACACACACACACACACAC 630 0.10068835675003555 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.5982278849211993E-4 0.0 5 0.0 0.0 0.0 1.5982278849211993E-4 0.0 6 0.0 0.0 0.0 1.5982278849211993E-4 0.0 7 0.0 0.0 0.0 1.5982278849211993E-4 0.0 8 0.0 0.0 0.0 1.5982278849211993E-4 0.0 9 0.0 0.0 0.0 1.5982278849211993E-4 0.0 10 0.0 0.0 0.0 1.5982278849211993E-4 0.0 11 0.0 0.0 0.0 1.5982278849211993E-4 0.0 12 0.0 0.0 0.0 1.5982278849211993E-4 0.0 13 0.0 0.0 0.0 1.5982278849211993E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 55 1.1371276E-5 15.529564 18 TTTAGGG 85 2.6223825E-7 13.439951 3 TAGGACC 320 0.0 13.3853035 4 AGGACCT 495 0.0 12.691252 5 GGACCTG 480 0.0 12.691252 6 TAGGCAG 60 4.0196336E-4 12.691252 5 GTCTTAA 85 3.7934442E-6 12.333799 1 ACTGTTC 225 0.0 12.23095 8 TCTCAAC 55 0.0030170912 12.116319 3 GGCGAGG 385 0.0 12.078548 19 GATATAC 255 0.0 11.960048 1 GCTCTAG 120 9.498763E-9 11.913329 1 AGACTCC 65 7.882344E-4 11.715001 6 TGTAGGA 1020 0.0 11.491014 2 GTCCTAA 440 0.0 11.480117 1 AGGACGT 865 0.0 11.444134 5 TGGCGAG 830 0.0 11.434082 18 CTAAATT 440 0.0 11.431484 19 GTAGGAC 935 0.0 11.301777 3 CTAGTAC 85 5.1984236E-5 11.199959 3 >>END_MODULE