FastQCFastQC Report
Thu 26 May 2016
SRR1926958_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926958_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646190
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10230.15831257060616846No Hit
GTACATGGGAAGCAGTGGTATCAAC8010.12395735000541636No Hit
CCCATGTACTCTGCGTTGATACCAC7080.10956529813212833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTT453.395071E-516.967861
CGCCTTG453.4155564E-516.9559672
TAGGACC1450.015.1017654
TCAATAC400.005137733614.3065963
GGACCTG1900.013.5230776
ATAGGAC852.56392E-713.4650323
TGGCGAG2500.013.27528718
CTGATCG500.001519031913.2752879
TATGGCG2650.013.23950316
CGTGAAA1403.6379788E-1212.8689019
ATGGCGA2750.012.41325517
GGCGAGG1302.0372681E-1012.39999219
ACGTGAA1501.2732926E-1112.0445448
TGTAGGA3250.012.0322142
GTCCTAT657.7046343E-411.7469781
GTTCTAT1156.602386E-811.6192951
GGCGAGA1800.011.58953719
GTAGGAC3250.011.1518093
GTCCCAA600.005668964211.1351571
ACTAGAC600.005698641811.1273523
Produced by FastQC (version 0.11.5)