##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926958_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 646190 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.207661833206952 30.0 26.0 31.0 10.0 31.0 2 26.85475788854671 31.0 27.0 31.0 10.0 34.0 3 26.447944103127565 31.0 26.0 31.0 10.0 34.0 4 30.132959346322288 35.0 30.0 37.0 10.0 37.0 5 29.663273959671304 35.0 30.0 37.0 10.0 37.0 6 29.506270601525866 35.0 29.0 37.0 8.0 37.0 7 29.574259892601248 35.0 30.0 37.0 2.0 37.0 8 29.250345873504696 35.0 28.0 37.0 2.0 37.0 9 31.443564586267197 37.0 31.0 39.0 2.0 39.0 10 31.649929587273093 37.0 32.0 39.0 2.0 39.0 11 31.835206363453473 37.0 32.0 39.0 2.0 39.0 12 31.732046302171188 37.0 32.0 39.0 2.0 39.0 13 31.819560810287996 37.0 32.0 39.0 2.0 39.0 14 32.72579581856729 38.0 32.0 40.0 2.0 41.0 15 32.275250313375324 38.0 31.0 40.0 2.0 41.0 16 32.33790526006283 38.0 32.0 40.0 2.0 41.0 17 32.55583342360606 38.0 32.0 40.0 2.0 41.0 18 32.603799192188056 38.0 32.0 40.0 2.0 41.0 19 31.97742769154583 38.0 31.0 40.0 2.0 41.0 20 32.26011544592148 38.0 32.0 40.0 2.0 41.0 21 32.22706170011916 38.0 32.0 40.0 2.0 41.0 22 32.23442640709389 38.0 32.0 40.0 2.0 41.0 23 32.19973382441697 38.0 32.0 40.0 2.0 41.0 24 30.90738791996162 38.0 29.0 40.0 2.0 41.0 25 31.429856543741003 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.132071904449461 1101 2 -1.1337770491428856 1101 3 -1.843519636852104 1101 4 -1.2317442589451417 1101 5 -1.4218463116317288 1101 6 -3.195434680558524 1101 7 -1.4901887185987768 1101 8 -1.318866869839848 1101 9 -1.5308841409257639 1101 10 -9.473503275445648 1101 11 -4.752586436608354 1101 12 -2.6676335858242197 1101 13 -1.8266773049513851 1101 14 -1.8919374813065915 1101 15 -3.060878451896464 1101 16 -1.5149276683869708 1101 17 -1.7229967528070063 1101 18 -1.3337376056869807 1101 19 -1.9109277140952763 1101 20 -1.2249993443861307 1101 21 -1.2309786957543913 1101 22 -1.2708732248151726 1101 23 -1.2616076199614241 1101 24 -1.390036911551853 1101 25 -1.2995712407979703 1102 1 -1.1573150561851548 1102 2 -0.6747851016331943 1102 3 -1.1997076956505168 1102 4 -0.9413675534500072 1102 5 -0.9653754608742169 1102 6 -1.448661147997754 1102 7 -0.557169431672218 1102 8 -0.23155646167964505 1102 9 -0.19978153121514097 1102 10 -0.2624016527175854 1102 11 -0.4028652591810733 1102 12 -0.4705385329670122 1102 13 -0.44810083418645164 1102 14 -0.8457735177562853 1102 15 -0.2229248958626755 1102 16 -0.33674298678539927 1102 17 -0.3835777422355662 1102 18 -0.8195497314113922 1102 19 -0.2434947055364205 1102 20 -0.21860755951399824 1102 21 -0.4346082168318297 1102 22 -0.6362520222842072 1102 23 -1.173333830999752 1102 24 -4.585429419495988 1102 25 -3.1105050764911226 1103 1 -0.9319224903931449 1103 2 -0.9722927428947195 1103 3 -0.5894999133525225 1103 4 -0.9041043468208656 1103 5 -1.2690947082796207 1103 6 -0.50887814696463 1103 7 -0.4255639736895134 1103 8 -0.2951600506076417 1103 9 -0.3849701299813937 1103 10 -0.6050731149099526 1103 11 -0.7885914255183692 1103 12 -1.0619400239239596 1103 13 -0.8430822508098927 1103 14 -0.885587383913105 1103 15 -0.7530591162825289 1103 16 -0.6245581218417513 1103 17 -0.8264986975111448 1103 18 -0.8617042027818478 1103 19 -2.3041789681814784 1103 20 -1.0227490589588548 1103 21 -0.8192691077193324 1103 22 -0.9145396938107027 1103 23 -1.0684910073553517 1103 24 0.39081086549198574 1103 25 -0.17952506594553697 1104 1 -0.8114141449427805 1104 2 -0.5345992485262379 1104 3 -1.6903850017030813 1104 4 -0.9336969824805976 1104 5 -0.9840251399976054 1104 6 -2.003245574700351 1104 7 -0.6176296230181002 1104 8 -0.30961712677715525 1104 9 -0.15883891995967758 1104 10 0.0033723668976186616 1104 11 0.050085711387534815 1104 12 0.1319553144841059 1104 13 -0.1401408198537517 1104 14 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25 -2.9482009954424058 1106 1 -0.8474387439426607 1106 2 -1.3358428041617998 1106 3 -0.8427692333925023 1106 4 -1.3497773855424953 1106 5 -0.6020697790608693 1106 6 -0.2592283939148672 1106 7 -3.439780863329311 1106 8 -1.0573455079360627 1106 9 -0.9666456277502675 1106 10 -0.5461521682604058 1106 11 -1.0515882011554005 1106 12 -0.5164016473133479 1106 13 -0.743994774804424 1106 14 -0.9737450640445893 1106 15 -0.2764137920270393 1106 16 -0.1666797610392976 1106 17 -0.4463145198909828 1106 18 -0.6750361699778225 1106 19 -0.11789359323950421 1106 20 -0.28719282498134646 1106 21 -0.9366342469607929 1106 22 -1.1724824582314675 1106 23 -1.0438273489789083 1106 24 -4.0591696879609245 1106 25 -2.4845765679299205 1107 1 0.03102225666305003 1107 2 -0.17041805284975808 1107 3 0.30522398587161703 1107 4 0.1769552997996513 1107 5 0.013903976076512947 1107 6 -0.0372751517230796 1107 7 -2.453870809709464 1107 8 -1.929023740066505 1107 9 -0.7659269022684434 1107 10 -0.19538120509508516 1107 11 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1108 23 0.36611944806622176 1108 24 -0.7958352063623799 1108 25 0.03588773411620494 1109 1 0.2798565839963487 1109 2 0.24120503998162945 1109 3 -1.2136650164647342 1109 4 0.08712292843405578 1109 5 0.4765704185176105 1109 6 0.6660954390912792 1109 7 0.7491974769869536 1109 8 1.0815048516968098 1109 9 0.27542069341281206 1109 10 0.22401270517865157 1109 11 -0.13338144238622007 1109 12 -0.22059978143056824 1109 13 -0.28754191316451383 1109 14 -0.16531774542886524 1109 15 -1.8747352329281526 1109 16 -0.2769351708532639 1109 17 -0.3503402426805309 1109 18 -0.08273176184507491 1109 19 -1.619695332469412 1109 20 -0.40774490893177173 1109 21 -0.04429531570674072 1109 22 0.14001205177513043 1109 23 0.2642639309709196 1109 24 -0.6808115994387407 1109 25 -0.5631626316199743 1110 1 0.5355708893389917 1110 2 0.7243442815811854 1110 3 0.984606903011894 1110 4 0.8259293673201853 1110 5 0.9619335529306348 1110 6 1.1319597639641366 1110 7 0.9004967760583362 1110 8 1.3009292697651027 1110 9 0.8946462762277925 1110 10 1.0692539082398902 1110 11 0.8223899814744051 1110 12 0.6595004017020045 1110 13 0.6714220629124448 1110 14 0.5856209807300417 1110 15 1.100555610247163 1110 16 0.9701770071680826 1110 17 0.2302969722911925 1110 18 0.7340375284131753 1110 19 -1.030604184269432 1110 20 0.41942054044964294 1110 21 0.5099721834639581 1110 22 0.7686317661040292 1110 23 0.7185131757698997 1110 24 -0.3785486461954477 1110 25 -0.11138754118232086 1111 1 0.8800587576082712 1111 2 0.7062957086575885 1111 3 0.8860887011032474 1111 4 0.9571109532332329 1111 5 1.1486169204131116 1111 6 1.0307073944480436 1111 7 0.9704734498615011 1111 8 1.574709244503044 1111 9 1.0242302955787643 1111 10 0.7761018816208072 1111 11 0.5086229252711085 1111 12 0.699051434705602 1111 13 0.39079181475774405 1111 14 0.6133316208875215 1111 15 -0.10576622993484364 1111 16 -0.06580226184219384 1111 17 0.09196610830189655 1111 18 0.3024089688681215 1111 19 0.8018219127039146 1111 20 0.2564946265208121 1111 21 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7 1.467470717761909 2215 8 0.44826679165201355 2215 9 0.8760166542275876 2215 10 0.6622475224123043 2215 11 0.3947197785698826 2215 12 0.20082803585587783 2215 13 0.4787400153277268 2215 14 0.6849746731803492 2215 15 0.6752672180337527 2215 16 1.09410841102018 2215 17 0.7781391805515696 2215 18 0.21721035774678654 2215 19 1.0999447909082392 2215 20 -2.8657401352121177 2215 21 -1.4702008518640888 2215 22 -0.13152530465506374 2215 23 0.1591610282778788 2215 24 0.1387806359139354 2215 25 0.3988993060728028 2216 1 0.7471642024633276 2216 2 -0.17299273541739524 2216 3 0.9573119667725578 2216 4 0.7233303368404158 2216 5 0.9296439247505965 2216 6 -0.5948798303210658 2216 7 -8.041485496302663 2216 8 -9.47865836437531 2216 9 -4.733700039467433 2216 10 -2.2901272871606473 2216 11 -1.3489899860377186 2216 12 -0.8976411763904224 2216 13 -0.7789507802887634 2216 14 -0.934767083171316 2216 15 -1.5959193267595992 2216 16 -0.9868660840410044 2216 17 -1.2220328180724422 2216 18 -0.9831004683380336 2216 19 -0.4960058700579033 2216 20 -0.879346454932989 2216 21 -3.666549566788653 2216 22 -2.3336666875239978 2216 23 -1.9559429080334887 2216 24 -2.6603618280892114 2216 25 -2.0319265330347704 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57574.0 3 12.0 4 430.0 5 2225.0 6 2667.0 7 2186.0 8 1779.0 9 1659.0 10 1491.0 11 1515.0 12 1449.0 13 1498.0 14 1487.0 15 1528.0 16 1596.0 17 1836.0 18 2075.0 19 2548.0 20 3302.0 21 4275.0 22 5050.0 23 6079.0 24 6956.0 25 8073.0 26 9132.0 27 9728.0 28 10973.0 29 12751.0 30 15124.0 31 18185.0 32 23298.0 33 30142.0 34 39925.0 35 55188.0 36 77402.0 37 113931.0 38 111121.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.896106514473594 19.414232430462395 13.577803798106375 25.111857256957638 2 14.007890023118449 20.850610190711713 41.48150214140062 23.659997644769216 3 18.766076398143042 25.608656307526388 29.65959873310235 25.965668561228224 4 11.61562895404825 17.794978774940027 38.85664992511991 31.73274234589182 5 12.544659166197114 37.88149918576356 35.642348963783505 13.931492684255826 6 29.79195368492748 37.4689246296497 20.156653921765916 12.58246776365691 7 26.397679406752566 31.61389251891147 24.531209744256717 17.457218330079247 8 25.265185198980074 36.06053128603376 21.776923184364044 16.89736033062212 9 26.120256642441973 16.13937325377108 20.95629493758078 36.784075166206165 10 14.209743266612273 29.844114546161077 34.9264540819881 21.01968810523855 11 32.849786165040655 23.88032671938631 24.63134171956431 18.638545396008723 12 22.326222867518105 26.038050098832432 31.91861842013612 19.717108613513343 13 27.710172025768266 22.943631146043902 26.08789194911975 23.258304879068085 14 20.730353861894642 22.767679550741978 28.41606518451558 28.085901402847796 15 23.152671235845144 30.617984484605337 23.901508819505068 22.327835460044454 16 21.539314085457598 29.13852031181311 27.89832638832992 21.423839214399376 17 21.380542260802805 29.034140512293433 27.937297611897787 21.648019615005975 18 21.057470552726485 28.63760892782516 29.28521677217639 21.01970374727197 19 22.970872704305936 27.74322282628887 27.922172410803846 21.363732058601347 20 22.793162747717275 28.033167762816753 28.360851885847666 20.812817603618303 21 22.54535942370856 27.545808297036505 27.70740269509738 22.20142958415756 22 22.262518380930267 28.45213218791115 27.718288058199832 21.567061372958747 23 21.921020614758792 28.141514019212373 27.908866606195883 22.028598759832953 24 22.610423864185147 28.03549537501393 27.96228190745075 21.391798853350174 25 22.25176879582578 27.7661087730106 27.985902260003314 21.996220171160306 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 309.0 1 309.0 2 377.5 3 446.0 4 446.0 5 446.0 6 643.5 7 841.0 8 841.0 9 841.0 10 1205.5 11 1570.0 12 1570.0 13 1570.0 14 2629.0 15 3688.0 16 3688.0 17 3688.0 18 6405.0 19 9122.0 20 9122.0 21 9122.0 22 13975.0 23 18828.0 24 18828.0 25 18828.0 26 26164.5 27 33501.0 28 33501.0 29 33501.0 30 41953.0 31 50405.0 32 50405.0 33 50405.0 34 58027.5 35 65650.0 36 65650.0 37 65650.0 38 71426.5 39 77203.0 40 77203.0 41 77203.0 42 79915.5 43 82628.0 44 82628.0 45 82628.0 46 83759.0 47 84890.0 48 84890.0 49 84890.0 50 80331.0 51 75772.0 52 75772.0 53 75772.0 54 66294.5 55 56817.0 56 56817.0 57 56817.0 58 47831.5 59 38846.0 60 38846.0 61 38846.0 62 31042.0 63 23238.0 64 23238.0 65 23238.0 66 17890.5 67 12543.0 68 12543.0 69 12543.0 70 9167.0 71 5791.0 72 5791.0 73 5791.0 74 4130.0 75 2469.0 76 2469.0 77 2469.0 78 1752.5 79 1036.0 80 1036.0 81 1036.0 82 712.0 83 388.0 84 388.0 85 388.0 86 266.0 87 144.0 88 144.0 89 144.0 90 93.0 91 42.0 92 42.0 93 42.0 94 29.0 95 16.0 96 16.0 97 16.0 98 11.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21588077809932063 2 0.12658815518655503 3 0.12906420712174438 4 0.0759838437611229 5 0.028784103746576084 6 0.027855584270880078 7 0.28969807641715284 8 0.28304368684133147 9 0.023986753122146737 10 0.04131911666847212 11 0.020272675219362726 12 0.02274872715455207 13 0.01918940249771739 14 0.02274872715455207 15 0.01887989600581872 16 0.02507002584379207 17 0.023831999876197402 18 0.018570389513920052 19 0.030641142697968093 20 0.021820207678856063 21 0.019808415481514727 22 0.02166545443290673 23 0.022903480400501403 24 0.020736934957210726 25 0.019963168727464058 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 646190.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.46282755202353 #Duplication Level Percentage of deduplicated Percentage of total 1 94.52935786391826 84.56863641183239 2 4.253076532888223 7.609845048546744 3 0.6245119260237356 1.6761180822613062 4 0.2201105027273023 0.7876683179152738 5 0.11083216274887858 0.49576793316103646 6 0.06361499608627592 0.3414706454753493 7 0.04401423314754769 0.2756346424939532 8 0.02783185016430393 0.19919328093623037 9 0.013013822443981244 0.10478280177886926 >10 0.09580926390310145 1.5436869352498412 >50 0.010211590961203018 0.6236943509850369 >100 0.006749885102627763 1.2100501667087715 >500 6.922959076242786E-4 0.40505326106790634 >1k 1.7307397690606964E-4 0.15839812158733496 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1023 0.15831257060616846 No Hit GTACATGGGAAGCAGTGGTATCAAC 801 0.12395735000541636 No Hit CCCATGTACTCTGCGTTGATACCAC 708 0.10956529813212833 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 1.5475324594933378E-4 0.0 13 0.0 0.0 0.0 1.5475324594933378E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCCTT 45 3.395071E-5 16.96786 1 CGCCTTG 45 3.4155564E-5 16.955967 2 TAGGACC 145 0.0 15.101765 4 TCAATAC 40 0.0051377336 14.306596 3 GGACCTG 190 0.0 13.523077 6 ATAGGAC 85 2.56392E-7 13.465032 3 TGGCGAG 250 0.0 13.275287 18 CTGATCG 50 0.0015190319 13.275287 9 TATGGCG 265 0.0 13.239503 16 CGTGAAA 140 3.6379788E-12 12.868901 9 ATGGCGA 275 0.0 12.413255 17 GGCGAGG 130 2.0372681E-10 12.399992 19 ACGTGAA 150 1.2732926E-11 12.044544 8 TGTAGGA 325 0.0 12.032214 2 GTCCTAT 65 7.7046343E-4 11.746978 1 GTTCTAT 115 6.602386E-8 11.619295 1 GGCGAGA 180 0.0 11.589537 19 GTAGGAC 325 0.0 11.151809 3 GTCCCAA 60 0.0056689642 11.135157 1 ACTAGAC 60 0.0056986418 11.127352 3 >>END_MODULE