FastQCFastQC Report
Thu 26 May 2016
SRR1926958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926958_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646190
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16560.25627137529209676No Hit
GGTATCAACGCAGAGTACTTTTTTT10400.16094337578730714No Hit
GTACATGGGAAGCAGTGGTATCAAC9590.1484083628654111No Hit
TATCAACGCAGAGTACTTTTTTTTT8810.13633760968136305No Hit
CCCATGTACTCTGCGTTGATACCAC8420.13030223308933905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTC751.4425783E-512.6944145
TAATAGT701.06696854E-412.2410424
TAGGACC1950.011.2296744
GACCTTG855.3610922E-511.1662717
GGCGAGG1451.2332748E-911.12518219
CCTACAC951.3230108E-511.0249523
GTCTAGA700.001448250410.8936161
CTTAACC700.001461458910.8817713
CTACACT803.688863E-410.7109124
GTCCTAC2450.010.5045581
CCAGAAC1105.865524E-610.3871463
GTAGGAC3800.010.2732513
TGGCGAG3350.010.1972718
CTCTGCG2700.010.1912369
GTTCTAA750.002571413810.1673751
CTCTTAT856.381904E-410.0926141
TGGAGTG856.4506865E-410.0808595
CTGCGTT2450.010.06931311
TGCGTTG2550.010.04575512
TAGGACG3050.09.9890484
Produced by FastQC (version 0.11.5)