##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926958_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 646190 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.75128213064269 31.0 30.0 33.0 25.0 34.0 2 30.08646218604435 31.0 30.0 34.0 26.0 34.0 3 30.14748758105201 31.0 30.0 34.0 26.0 34.0 4 33.72432721026323 35.0 35.0 37.0 28.0 37.0 5 33.659111097355265 37.0 35.0 37.0 28.0 37.0 6 33.602910908556304 37.0 35.0 37.0 28.0 37.0 7 33.58193101100296 37.0 35.0 37.0 28.0 37.0 8 33.58982497407883 37.0 35.0 37.0 28.0 37.0 9 35.10892616722636 39.0 35.0 39.0 29.0 39.0 10 34.99472910444297 39.0 35.0 39.0 28.0 39.0 11 35.02292824091985 39.0 35.0 39.0 28.0 39.0 12 34.92356737182563 39.0 35.0 39.0 27.0 39.0 13 34.93711292344357 39.0 35.0 39.0 27.0 39.0 14 36.02026184249215 40.0 36.0 41.0 27.0 41.0 15 35.99170832108204 40.0 36.0 41.0 27.0 41.0 16 35.96402915551154 40.0 36.0 41.0 27.0 41.0 17 35.94142280134326 40.0 36.0 41.0 27.0 41.0 18 35.90359956050078 40.0 36.0 41.0 27.0 41.0 19 35.98693108837958 40.0 36.0 41.0 27.0 41.0 20 35.943408285488786 40.0 36.0 41.0 27.0 41.0 21 35.89633853820084 40.0 36.0 41.0 27.0 41.0 22 35.80514864049273 40.0 36.0 41.0 27.0 41.0 23 35.74077593277519 40.0 36.0 41.0 27.0 41.0 24 35.664395920704436 40.0 36.0 41.0 27.0 41.0 25 35.54520651820672 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1527257901668726 1101 2 -0.0698514254375624 1101 3 0.10221190586804241 1101 4 -0.02257272011460998 1101 5 0.012555929889323636 1101 6 -0.2974757400621044 1101 7 -0.1770598635613041 1101 8 -0.17771859622477137 1101 9 -0.1715886647019218 1101 10 -0.1218872265568649 1101 11 -0.2512739851835022 1101 12 -0.10310276918604444 1101 13 -0.17478077771998812 1101 14 -0.10001346266822253 1101 15 -0.23626594115660993 1101 16 -0.20644816574353797 1101 17 -0.04457715659489736 1101 18 -0.10759625935664019 1101 19 -0.2855237043297123 1101 20 0.09658568522874589 1101 21 0.11576194925828531 1101 22 -0.03333847300660864 1101 23 -0.27340805858954553 1101 24 -0.012282993967723144 1101 25 -0.2308667909876405 1102 1 -0.47700320092541304 1102 2 -0.42095008721795324 1102 3 -0.2253847701463485 1102 4 -0.3180044927996519 1102 5 -0.2645855540604103 1102 6 -0.5859836155392344 1102 7 -0.22681343976386614 1102 8 -0.19190110646471936 1102 9 -0.3093295903628288 1102 10 -0.16266569832165345 1102 11 -0.35746472599974055 1102 12 -0.1873224200087833 1102 13 -0.16704798393711684 1102 14 -0.1606710505257638 1102 15 -0.23588831304734725 1102 16 -0.10139674609786198 1102 17 -0.09906824907731249 1102 18 -0.17832643334450182 1102 19 -0.12394607707869199 1102 20 -0.09372922657577476 1102 21 -0.10850301693756847 1102 22 -0.29780688634632213 1102 23 -0.37058292829711803 1102 24 -0.22835278650827462 1102 25 -0.1485374263254755 1103 1 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23 -0.47800214981402434 1105 24 -0.5351298071625621 1105 25 -0.4053817403044704 1106 1 -0.08816911429720875 1106 2 0.010201029543463136 1106 3 -0.02558588499440617 1106 4 0.1631851036525589 1106 5 0.123249336176805 1106 6 0.0255734725898904 1106 7 0.05633968389413013 1106 8 0.21143833543094814 1106 9 -0.18831627766537906 1106 10 0.14210408257795848 1106 11 -0.15123484279941835 1106 12 -0.054620635486550384 1106 13 0.04126320408612827 1106 14 0.011662689572624174 1106 15 -0.025285351174126447 1106 16 -0.10154343663185728 1106 17 -0.2291785008322833 1106 18 -0.12831346505091545 1106 19 -0.3450703643540365 1106 20 -0.23885931344208444 1106 21 -0.23871106238186712 1106 22 -0.4844418905344412 1106 23 -0.4039008130621369 1106 24 -0.2821435675397055 1106 25 -0.10044206472267803 1107 1 0.5458154623742928 1107 2 0.6095379971560995 1107 3 0.7598493545062261 1107 4 0.6718175332942948 1107 5 0.7827185850517608 1107 6 0.8034108731451965 1107 7 0.9244872990507815 1107 8 0.7390491016784964 1107 9 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21 0.1984617072136814 1108 22 0.22236268882780763 1108 23 0.13065089009720765 1108 24 0.09419934877190883 1108 25 0.10113992242077785 1109 1 0.8633749271959061 1109 2 0.5780563265955152 1109 3 0.862833957016349 1109 4 0.8143759470585437 1109 5 0.9114550939797468 1109 6 0.9498912323865909 1109 7 0.771648543262458 1109 8 0.6424809541220498 1109 9 0.8149000924224836 1109 10 0.79416617516938 1109 11 0.8118846582699817 1109 12 1.0215631092302928 1109 13 0.6684962269837484 1109 14 0.7763917034983123 1109 15 0.8508164777341847 1109 16 0.8169993608032158 1109 17 0.9513345250069491 1109 18 0.6942244133500708 1109 19 0.690137091005937 1109 20 0.9881751504383516 1109 21 0.8577412272773373 1109 22 0.7945222932593055 1109 23 1.0745855625616798 1109 24 0.9375583079441796 1109 25 0.8917064786671602 1110 1 0.15302960551657918 1110 2 -0.039631374472463676 1110 3 0.18479789187376028 1110 4 0.3924408658854759 1110 5 0.1756724034423769 1110 6 0.33847577270029205 1110 7 0.27724753200952534 1110 8 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0.463735834073816 2214 9 0.5074436967704941 2214 10 0.20392190739440963 2214 11 0.5153997403783066 2214 12 0.5416991316643234 2214 13 0.5609040743443501 2214 14 0.5883512115119558 2214 15 0.6282110432833861 2214 16 0.48735541952759576 2214 17 0.4476855819585168 2214 18 0.37599278083438037 2214 19 0.6028023078284264 2214 20 0.5003250216019381 2214 21 0.3729702792726428 2214 22 0.3750208778599884 2214 23 0.5837406269988463 2214 24 0.36891191507938714 2214 25 0.39805065296700093 2215 1 0.26863110927597944 2215 2 0.2413064152072124 2215 3 0.22603540346094775 2215 4 0.43176005426738584 2215 5 0.42356096045133995 2215 6 0.2561294960497591 2215 7 0.09790362429502864 2215 8 0.2706775740570464 2215 9 -0.008244152341887911 2215 10 0.29496650850020245 2215 11 -0.01571656645683106 2215 12 0.14809401774530784 2215 13 0.17840659719211516 2215 14 0.09710478258519828 2215 15 0.13222272655583822 2215 16 0.241285376745779 2215 17 0.1878429223795166 2215 18 0.13276183011163312 2215 19 -0.07825643543805683 2215 20 -0.09243227333672621 2215 21 0.08950701335737676 2215 22 0.12492138527218088 2215 23 0.1439798356063804 2215 24 0.13649722997342906 2215 25 0.06278550522051063 2216 1 -0.21998626749929073 2216 2 -0.01124285011295001 2216 3 -1.8363448117830465 2216 4 -1.1941060816606708 2216 5 -0.46885390022977447 2216 6 -0.263969771727524 2216 7 -0.10646759787805138 2216 8 -0.0861212858377911 2216 9 -0.05464692397685411 2216 10 -0.1858495021145714 2216 11 -0.15662029636985153 2216 12 -0.18571231180405334 2216 13 -0.30039457239853107 2216 14 -0.3761555741890916 2216 15 -0.2878347701270485 2216 16 -0.2316985536684939 2216 17 -0.34816621811879145 2216 18 -0.23642093071199355 2216 19 -0.3532208071516578 2216 20 -0.3015764573261137 2216 21 0.011180068544362598 2216 22 -0.19864488334052766 2216 23 -0.25578182344320766 2216 24 -0.3188814127117183 2216 25 -0.23350669587045303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16901.0 3 15.0 4 267.0 5 1974.0 6 2647.0 7 1835.0 8 1557.0 9 1295.0 10 1119.0 11 1027.0 12 945.0 13 916.0 14 875.0 15 893.0 16 859.0 17 940.0 18 949.0 19 1262.0 20 1607.0 21 2034.0 22 2634.0 23 3160.0 24 3801.0 25 4482.0 26 5431.0 27 5896.0 28 6657.0 29 8343.0 30 10456.0 31 13353.0 32 17475.0 33 23069.0 34 30951.0 35 43305.0 36 63556.0 37 109280.0 38 254424.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.640337613405386 20.350069406573795 11.296074810371325 27.713518169649497 2 13.984449673672419 21.87130625529614 39.93341771014551 24.210826360885925 3 17.396795522646787 26.307700403655183 29.90592729253857 26.38957678115946 4 11.886442068122378 18.902644732973272 38.236122502669495 30.974790696234855 5 12.736608987543352 38.84929607326966 35.392572439376565 13.02152249981042 6 30.013494480307223 38.74981171395429 18.650645141754158 12.586048663984334 7 27.037016972239208 32.62431217156434 22.602874368281338 17.735796487915113 8 24.707528349514263 37.29135423002456 20.727685252680956 17.273432167780218 9 26.17991144183282 16.28715008922367 20.526086895960404 37.0068515729831 10 14.434660146093847 30.33304444719574 34.11647270026 21.115822706450416 11 32.87080384488599 24.0864062449219 24.443440216541802 18.599349693650304 12 22.367777598950145 26.193622869028015 31.739411684597574 19.699187847424263 13 27.883673459912934 22.92623893305436 25.99735987285296 23.19272773417974 14 20.714250342005734 22.971333419995418 28.14041733984537 28.173998898153478 15 22.759954810653543 30.72829131652661 23.903926211368525 22.60782766145132 16 21.72731521459898 29.28509305427883 27.50943232711527 21.47815940400692 17 21.23905309144257 29.142957069702735 27.993289868103194 21.6246999707515 18 21.084926537724666 28.70305889052929 29.206477432054744 21.005537139691295 19 22.535032537121722 27.95979479560807 28.19471204066947 21.31046062660074 20 22.618929709987928 28.03940078615866 28.527190566096134 20.81447893775728 21 22.613892096196526 27.497237244116178 27.919157274328505 21.969713385358787 22 22.235255884492176 28.40526780049212 27.79848032312478 21.56099599189093 23 21.65682176576822 28.17890740944213 27.9443011255221 22.21996969926755 24 21.876450816171143 28.05468377623849 28.55051471088909 21.51835069670127 25 22.101970762025918 27.83875470868813 28.11531759076122 21.94395693852473 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 266.0 1 266.0 2 288.0 3 310.0 4 310.0 5 310.0 6 518.0 7 726.0 8 726.0 9 726.0 10 1092.0 11 1458.0 12 1458.0 13 1458.0 14 2480.0 15 3502.0 16 3502.0 17 3502.0 18 6180.0 19 8858.0 20 8858.0 21 8858.0 22 13866.0 23 18874.0 24 18874.0 25 18874.0 26 26010.0 27 33146.0 28 33146.0 29 33146.0 30 42221.5 31 51297.0 32 51297.0 33 51297.0 34 58514.5 35 65732.0 36 65732.0 37 65732.0 38 71021.0 39 76310.0 40 76310.0 41 76310.0 42 79233.5 43 82157.0 44 82157.0 45 82157.0 46 83943.5 47 85730.0 48 85730.0 49 85730.0 50 81616.0 51 77502.0 52 77502.0 53 77502.0 54 67234.5 55 56967.0 56 56967.0 57 56967.0 58 47956.0 59 38945.0 60 38945.0 61 38945.0 62 31102.5 63 23260.0 64 23260.0 65 23260.0 66 17794.0 67 12328.0 68 12328.0 69 12328.0 70 8865.5 71 5403.0 72 5403.0 73 5403.0 74 3811.0 75 2219.0 76 2219.0 77 2219.0 78 1528.5 79 838.0 80 838.0 81 838.0 82 547.0 83 256.0 84 256.0 85 256.0 86 172.0 87 88.0 88 88.0 89 88.0 90 52.0 91 16.0 92 16.0 93 16.0 94 9.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11111283059162166 2 0.10414893452390164 3 0.014546805119237376 4 0.0 5 0.0044878441325306795 6 0.344016465745369 7 0.02089168820316006 8 0.009130441511010693 9 0.00943994800290936 10 0.004642597378480013 11 0.005106857116328015 12 9.285194756960027E-4 13 0.0013927792135440041 14 3.095064918986676E-4 15 0.003095064918986676 16 0.0012380259675946704 17 4.6425973784800135E-4 18 0.0012380259675946704 19 7.73766229746669E-4 20 0.001547532459493338 21 0.014856311611136043 22 0.0 23 1.547532459493338E-4 24 3.095064918986676E-4 25 0.006809142821770687 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 646190.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.99302304381148 #Duplication Level Percentage of deduplicated Percentage of total 1 92.3663657256779 79.42863016321337 2 5.91751430100963 10.177298872976102 3 0.9089051464765118 2.344785036167806 4 0.3083090578566372 1.0604971166752644 5 0.14337537411643578 0.6164640925154877 6 0.08511475141951572 0.439156486811202 7 0.0596915781018362 0.3593141475862871 8 0.037215453791859275 0.2560215500407405 9 0.026002724969295746 0.20124476347378872 >10 0.12694575771649666 1.9477969194206555 >50 0.010460468087005388 0.663322397296874 >100 0.009017554265892694 1.5884251258149598 >500 7.214043407566236E-4 0.4989224397429098 >1k 3.607021703783118E-4 0.4181208882644963 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1656 0.25627137529209676 No Hit GGTATCAACGCAGAGTACTTTTTTT 1040 0.16094337578730714 No Hit GTACATGGGAAGCAGTGGTATCAAC 959 0.1484083628654111 No Hit TATCAACGCAGAGTACTTTTTTTTT 881 0.13633760968136305 No Hit CCCATGTACTCTGCGTTGATACCAC 842 0.13030223308933905 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.5475324594933378E-4 0.0 8 0.0 0.0 0.0 1.5475324594933378E-4 0.0 9 0.0 0.0 0.0 3.0950649189866755E-4 0.0 10 0.0 0.0 0.0 3.0950649189866755E-4 0.0 11 0.0 0.0 0.0 3.0950649189866755E-4 0.0 12 0.0 0.0 0.0 3.0950649189866755E-4 0.0 13 0.0 0.0 0.0 3.0950649189866755E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACACTC 75 1.4425783E-5 12.694414 5 TAATAGT 70 1.06696854E-4 12.241042 4 TAGGACC 195 0.0 11.229674 4 GACCTTG 85 5.3610922E-5 11.166271 7 GGCGAGG 145 1.2332748E-9 11.125182 19 CCTACAC 95 1.3230108E-5 11.024952 3 GTCTAGA 70 0.0014482504 10.893616 1 CTTAACC 70 0.0014614589 10.881771 3 CTACACT 80 3.688863E-4 10.710912 4 GTCCTAC 245 0.0 10.504558 1 CCAGAAC 110 5.865524E-6 10.387146 3 GTAGGAC 380 0.0 10.273251 3 TGGCGAG 335 0.0 10.19727 18 CTCTGCG 270 0.0 10.191236 9 GTTCTAA 75 0.0025714138 10.167375 1 CTCTTAT 85 6.381904E-4 10.092614 1 TGGAGTG 85 6.4506865E-4 10.080859 5 CTGCGTT 245 0.0 10.069313 11 TGCGTTG 255 0.0 10.045755 12 TAGGACG 305 0.0 9.989048 4 >>END_MODULE